公开(公告)号：KR1020110019495A

公开(公告)日：2011-02-28

申请号：KR1020090077040

申请日：2009-08-20

Applicant: 서울대학교산학협력단

Inventor： 이현숙 ,  최은희 ,  박인애

IPC: C07K16/18 ,  A61K39/395

CPC classification number: C07K16/18 ,  A61K39/39533 ,  C07K2317/732

Abstract: PURPOSE: An antibody which specifically recoganizes acetylated BubR1 and a method and kit for detecting acetylation of BubR1 are provided to measure cell division check point activity. CONSTITUTION: An antibody specifically recognizes acetylated BubR1 protein. BubR1 is derived from Homo sapiens, Mus musculus, Gallus gallus, or Xenopus laevis. In homo sapiens-derived BubR1, 250th lysine residue is acetylated. In Mus musculus-derived BubR1, 237th lysine residue is acetylate. The antibody is Fv, ScFv, Fab, Fab' or F(ab')2. A kit for detecting acetylation of BubR1 in a sample contains the antibody for BubR1. The sample is derived from cancer tissue. A method for detecting acetylation of BubR1 comprises: a step of isolating the test sample; a step of contacting the sample to the antibody; and a step of determining acetylation of BubR1.

Abstract translation: 目的：提供特异性重新配位乙酰化BubR1的抗体以及用于检测BubR1乙酰化的方法和试剂盒，以测定细胞分裂检查点活性。 构成：抗体特异性识别乙酰化的BubR1蛋白。 BubR1衍生自智人，小鼠，马鞭草或非洲爪蟾。 在来自人来源的BubR1中，第250位赖氨酸残基被乙酰化。 在Mus musculus来源的BubR1中，第237位赖氨酸残基是乙酰化物。 抗体是Fv，ScFv，Fab，Fab'或F（ab'）2。 用于检测样品中BubR1乙酰化的试剂盒含有BubR1抗体。 样品来源于癌组织。 检测BubR1乙酰化的方法包括：分离测试样品的步骤; 使样品与抗体接触的步骤; 以及确定BubR1乙酰化的步骤。