公开(公告)号：KR1020140041664A

公开(公告)日：2014-04-04

申请号：KR1020140030553

申请日：2014-03-14

Applicant: 서울대학교산학협력단

Inventor： 정종훈 ,  백수정 ,  정연훈 ,  정필훈

IPC: A61L24/00 ,  A61L27/36 ,  A61L27/56 ,  A61F2/28

CPC classification number: A61L24/0084 ,  A61F2/28 ,  A61L24/0005 ,  A61L27/3608 ,  A61L27/56

Abstract: The present invention relates to nanoceramic bone cement containing animal bone powder and a preparation method thereof, and a porous bone support containing nanohorse bone powder. The nanoceramic bone cement containing animal bone powder of the present invention is a hydroxyapatite component similar to a real bone and has a strong strength, fast curing time, biocompatibility, and excellent characteristics for promoting bone tissue regeneration.

Abstract translation: 本发明涉及含有动物骨粉的纳米陶瓷骨水泥及其制备方法，以及含有纳米骨粉末的多孔骨架。 本发明的含有动物骨粉的纳米陶瓷骨粘固剂是与实骨相似的羟基磷灰石成分，具有强度高，固化时间快，生物相容性好，促进骨组织再生的优异特性。

公开(公告)号：KR1020120094547A

公开(公告)日：2012-08-27

申请号：KR1020110006785

申请日：2011-01-24

Applicant: 서울대학교산학협력단

Inventor： 정종훈 ,  백수정 ,  정연훈 ,  정필훈

IPC: A61L24/00 ,  A61L27/36 ,  A61L27/38 ,  A61L27/56 ,  A61F2/28

CPC classification number: A61L24/0084 ,  A61F2/28 ,  A61L27/3608 ,  A61L27/38 ,  A61L27/56

Abstract: PURPOSE: Nano ceramic bone cement using animal bone is provided to have strong strength, quick curing time, bio-compatibility, and excellent accelerating effect of bone tissue regeneration by a hydroxyapatite component which is similar with rear bone. CONSTITUTION: A manufacturing method of nano ceramic bone cement using animal bone comprises: a step of removing blood and organic material in animal bone; a step of sintering dried animal bone; a step of pulverizing the sintered animal bone; a step of manufacturing micro animal bone powder; a step of sintering the pulverized animal bone powder 1 times to 5 times; a step of grinding the animal bone powder to manufacture a nano animal bone powder; and a step of mixing the nano animal bone powder with chitosan solution.

Abstract translation: 目的：提供使用动物骨骼的纳米陶瓷骨水泥，具有强度高，固化时间快，生物相容性好，骨组织再生由于与后骨相似的羟基磷灰石组分的优异加速作用。 构成：使用动物骨的纳米陶瓷骨水泥的制造方法包括：去除动物骨骼中的血液和有机物质的步骤; 烧结干燥动物骨的步骤; 粉碎烧结的动物骨的步骤; 制造微型动物骨粉的一步; 将粉碎的动物骨粉烧结1次至5次的步骤; 研磨动物骨粉以制造纳米动物骨粉的步骤; 以及将纳米动物骨粉与壳聚糖溶液混合的步骤。

公开(公告)号：KR101782673B1

公开(公告)日：2017-09-29

申请号：KR1020140077677

申请日：2014-06-24

Applicant: 서울대학교산학협력단

Inventor： 정종훈 ,  김장호 ,  정필훈

IPC: C12M3/00 ,  B82Y99/00 ,  C12N5/0775 ,  C12N5/071 ,  C12M1/12

Abstract: 본발명은마루와골로구성된나노구조를형성하고, 줄기세포의골분화를촉진시킬수 있는효과를나타내는배양지지체, 상기배양지지체를포함하는골분화촉진용키트및 상기배양지지체를이용하여줄기세포를골세포로분화시키는방법에관한것이다. 본발명의마루와골로구성된나노구조가형성된배양지지체를이용하면, 줄기세포의골분화를촉진시킬수 있으므로, 줄기세포에서분화된골조직을이용한각종골질환의치료에널리활용될수 있을것이다.

Abstract translation: 本发明涉及形成由地板和骨组成的具有促进骨细胞分化的作用的纳米结构的培养支持体，含有上述培养支持体的骨形态发生用试剂盒和培养支持体， 。“ 使用本发明的具有由地板和骨形成的纳米结构的培养支持体可以促进干细胞的骨分化，因此其可以广泛用于使用从干细胞分化的骨组织来治疗各种骨疾病。

公开(公告)号：KR1020150014399A

公开(公告)日：2015-02-06

申请号：KR1020140095959

申请日：2014-07-28

Applicant: 서울대학교산학협력단

Inventor： 정종훈 ,  팬디,샴하비 ,  가르그,반가지 ,  정필훈

IPC: A61K47/48 ,  A61K48/00 ,  A61K9/00 ,  C12N15/11 ,  C12N15/113

CPC classification number: A61K48/0075 ,  A61K47/551 ,  A61K47/59 ,  A61K47/6921 ,  A61K48/0041 ,  C12N15/111 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2310/351 ,  C12N2320/32 ,  C12Y201/02001 ,  Y10T428/2982 ,  A61K9/0019 ,  A61K9/0073

Abstract: The present invention relates to a vitamin B6-coupled gene carrier (vitamin B6 PEA, VBPEA) and to a method for manufacturing the gene carrier. In addition, the present invention relates to a gene carrying complex in which a curing gene is combined with the gene carrier and to a pharmaceutical formulation for gene therapy, including the complex as an active ingredient. Moreover, the present invention relates to gene therapy using the gene carrier, the gene carrying complex or the pharmaceutical formulation including the same. The vitamin B6-coupled gene carrier (VBPEA) according to the present invention has a gene-carrying rate much higher than that of existing gene carriers, has little cytotoxicity when combined with DNA, and has very high in vivo transgenic efficiency. In addition, the vitamin B6-coupled gene carrier (VBPEA) can be used for anticancer treatment as gene silencing efficiency via siRNA is high and the apoptosis of cancer cells and the inhibition of cancer cell proliferation can be induced using the same. Accordingly, the gene carrier according to the present invention can be used widely in a gene therapy field not only as an experimental gene carrier but also for various diseases according to curing genes constituting the same.

Abstract translation: 本发明涉及维生素B6偶联基因载体（维生素B6 PEA，VBPEA）及其制备方法。 此外，本发明涉及其中固化基因与基因载体组合的基因携带复合物和用于基因治疗的药物制剂，包括该复合物作为活性成分。 此外，本发明涉及使用基因载体，携带基因的基因或包含其的药物制剂的基因治疗。 本发明的维生素B6偶联基因载体（VBPEA）具有比现有基因携带者高得多的基因携带率，与DNA结合时细胞毒性小，具有非常高的体内转基因效率。 此外，维生素B6偶联基因载体（VBPEA）可用于抗癌治疗，因为通过siRNA的基因沉默效率高，并且可以使用其诱导癌细胞的凋亡和癌细胞增殖的抑制。 因此，本发明的基因载体不仅可以广泛用于基因治疗领域，不仅可以作为实验基因载体，而且可以根据构成其的固化基因用于各种疾病。

公开(公告)号：KR1020120139399A

公开(公告)日：2012-12-27

申请号：KR1020110059186

申请日：2011-06-17

Applicant: 서울대학교산학협력단

Inventor： 임기택 ,  정종훈 ,  정필훈

IPC: C12M3/02 ,  C12M1/42 ,  C12M1/34

CPC classification number: C12M35/04 ,  C12M27/02 ,  C12M29/10 ,  C12M41/00 ,  C12M41/36

Abstract: PURPOSE: A novel transonic stimulated perfusion flow culture chamber system for culturing adult stem cells in vitro to be similar to in vivo is provided to use a three-dimensional culture without applied stress and to optimize cell proliferation and differentiation. CONSTITUTION: A perfusion flow culture system comprises: a fresh medium container(8) for feeding fresh media; a control-based vessel(6), which includes a stirrer and a pH, DO measuring unit and controls the fresh media before circulating the media; a culture chamber(3) which provides a space for culturing cells and directly cultures the cells; a sensor(4) for measuring a cell environment; a 4-gas mixture control unit(1)(2) for CO_2, O_2, and N_2 which maintains the pH and DO of the media and optimizes a cell culture environment; and a waste vessel(9) which selectively discharges used media. [Reference numerals] (1) Gas mass flowmeter; (10) Cell stimulator; (11) Load cell transmitter; (2) Gas exchanger; (3) Culture chamber; (4,7) Sensor; (5) Inverted microscope; (6) Control-based vessel; (8) Fresh medium container; (9) Waste medium container; (AA) Medium line; (BB) Gas line

Abstract translation: 目的：提供一种用于体外培养成体干细胞与体内相似的新型跨音刺激灌流流动培养室系统，以使用不施加应力的三维培养，并优化细胞增殖和分化。 构成：灌注流培养系统包括：用于喂养新鲜培养基的新鲜培养基容器（8） 基于对照的容器（6），其包括搅拌器和pH，DO测量单元，并且在循环媒介之前控制新鲜介质; 培养室（3），其提供用于培养细胞并直接培养细胞的空间; 用于测量细胞环境的传感器（4）; 用于CO_2，O_2和N_2的4-气体混合物控制单元（1）（2），其保持介质的pH和DO并优化细胞培养环境; 和废物容器（9），其选择性地排出用过的介质。 （附图标记）（1）气体质量流量计; （10）细胞刺激器; （11）称重传感器; （2）气体交换器; （3）文化室; （4,7）传感器; （5）倒置显微镜; （6）基于控制的船舶; （8）新鲜容器; （9）废物容器; （AA）中线; （BB）气体管线