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    돼지 유행성 설사 바이러스 항원 진단키트 및 진단 방법
    12.
    发明公开
    돼지 유행성 설사 바이러스 항원 진단키트 및 진단 방법 无效
    PORCINE流行病毒病毒诊断方法的测试工具

    公开(公告)号：KR1020080040942A

    公开(公告)日：2008-05-09

    申请号：KR1020060108942

    申请日：2006-11-06

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 바이오노트

    Inventor： 탁동섭 ,  김성희 ,  권준헌 ,  조수동 ,  양동군 ,  오진식 ,  하건우 ,  조영식

    IPC: G01N33/53 ,  G01N33/50 ,  G01N33/48

    Abstract: A kit and a method for diagnosing a porcine epidemic diarrhea virus antigen are provided to diagnose the antigen conveniently and rapidly without a special testing equipment in a farm or a laboratory field. A kit for diagnosing a porcine epidemic diarrhea virus antigen is characterized in that a conjugate of a monoclonal antibody 6G15(deposition no. KCTC 10996BP) coupled to nucleocapside 58kDa of an epidemic diarrhea virus and a gold particle is attached to a reaction position of the porcine epidemic diarrhea virus antigen on a strip and a monoclonal antibody 9D11(deposition no. KCTC 10997BP) is attached to a position different from the coupling portion of the porcine epidemic diarrhea virus antigen and the monoclonal antibody 6G15. A method for diagnosing the porcine epidemic diarrhea virus antigen comprises the steps of: (a) loading a sample onto a detection pad on a strip; (b) reacting the conjugate of the monoclonal antibody 6G15 coupled to nucleocapside 58kDa of the epidemic diarrhea virus and the gold particle with the porcine epidemic diarrhea virus antigen in the sample; and (c) reacting the porcine epidemic diarrhea virus antigen, the monoclonal antibody 9D11 and the reaction product obtained from the step(b).

    Abstract translation: 提供了一种用于诊断猪流行性腹泻病毒抗原的试剂盒和方法，用于在农场或实验室领域中没有特殊检测设备的情况下方便快速地诊断抗原。 一种用于诊断猪流行性腹泻病毒抗原的试剂盒的特征在于，将与流行性腹泻病毒和金颗粒的核衣壳58kDa偶联的单克隆抗体6G15（沉积编号KCTC 10996BP）的缀合物连接到猪的反应位置 将条带上的流行性腹泻病毒抗原和单克隆抗体9D11（沉积编号KCTC 10997BP）连接到与猪流行性腹泻病毒抗原和单克隆抗体6G15的偶联部分不同的位置。 用于诊断猪流行性腹泻病毒抗原的方法包括以下步骤：（a）将样品加载到条带上的检测垫上; （b）将与流行性腹泻病毒的金卡帕58kDa和金颗粒偶联的单克隆抗体6G15的缀合物与样品中的猪流行性腹泻病毒抗原反应; 和（c）使猪流行性腹泻病毒抗原，单克隆抗体9D11和从步骤（b）获得的反应产物反应。

    신속 면역크로마토그라피법에 의한 Ｈ5형 고병원성 및 일반조류인플루엔자 바이러스 항원 진단 스트립 및 그 제조방법
    13.
    发明公开
    신속 면역크로마토그라피법에 의한 Ｈ5형 고병원성 및 일반조류인플루엔자 바이러스 항원 진단 스트립 및 그 제조방법 有权
    用于H5型高致病性流感病毒性流行性感冒病毒和使用快速免疫印迹的一般流行性流感病毒抗原的诊断条带及其制备方法

    公开(公告)号：KR1020070072945A

    公开(公告)日：2007-07-10

    申请号：KR1020060000325

    申请日：2006-01-03

    Applicant: 대한민국(농림축산식품부 농림축산검역본부장) ,  주식회사 바이오노트

    Inventor： 이윤정 ,  최준구 ,  이은경 ,  권용국 ,  권준헌 ,  김재홍 ,  성환우 ,  오진식 ,  조영식 ,  조병기

    IPC: G01N33/53

    Abstract: A diagnostic strip for a H5 type highly pathogenic avian influenza and general avian influenza is provided to conveniently and rapidly diagnose whether a bird is infected by the H5 type highly pathogenic avian influenza and general avian influenza or not from stool in situ without special testing equipment. The diagnostic strip is characterized in that a monoclonal antibody common to general avian influenza virus in which hemaglutinin antigens are H1 to H15 and a monoclonal antibody specific to a highly pathogenic avian influenza in which a hemaglutinin antigen is H5 are used for diagnosing the highly pathogenic and the general avian influenza in which the hemaglutinin antigen is H5 from stool or tissue of chicken. The method for preparing the diagnostic strip comprises the steps of: (a) respectively absorbing the monoclonal antibody specific to the highly pathogenic avian influenza virus in which the hemaglutinin antigen is H5 and the monoclonal antibody common to general avian influenza virus into a membrane; (b) respectively attaching the antibody absorbed membrane obtained from the step(a) to gold particles so as to prepare an antibody-gold conjugate and then mixing it; and (c) precipitating the conjugate obtained from the step(b) in a basic material and then drying it.

    Abstract translation: 提供一种H5型高致病性禽流感和一般禽流感的诊断条，以方便快捷地诊断鸟类是否由H5型高致病性禽流感和一般禽流感感染，或者不是在没有特殊检测设备的情况下从粪便中感染。 诊断条的特征在于，其中血凝素抗原为H1至H15的一般性禽流感病毒共有的单克隆抗体和其中血凝素抗原为H5的高致病性禽流感特异性的单克隆抗体用于诊断高致病性和 其中血凝素抗原为粪便或鸡组织中H5的一般性禽流感。 制备诊断条的方法包括以下步骤：（a）分别将其中血凝素抗原为H5的高致病性禽流感病毒和一般禽流感病毒共有的单克隆抗体特异性的单克隆抗体吸收到膜中; （b）将由步骤（a）获得的抗体吸收膜分别附着于金颗粒上，制备抗体 - 金缀合物，然后混合; 和（c）将由步骤（b）获得的缀合物沉淀在碱性材料中，然后干燥。

    인간 락토페린을 대량생산하기 위한 형질전환 제브라피쉬및 이를 이용한 인간 락토페린의 대량생산방법
    15.
    发明公开
    인간 락토페린을 대량생산하기 위한 형질전환 제브라피쉬및 이를 이용한 인간 락토페린의 대량생산방법 无效
    用于生产人LACTOFERRIN的转基因青霉素和使用其生产人LACTOFERRIN的方法

    公开(公告)号：KR1020100007241A

    公开(公告)日：2010-01-22

    申请号：KR1020080067786

    申请日：2008-07-11

    Applicant: 서울대학교산학협력단 ,  주식회사 바이오노트

    Inventor： 박재학 ,  석승혁 ,  조영식 ,  하건우 ,  오진식 ,  오연경

    IPC: C12N15/11 ,  C12N15/12 ,  C12N15/85

    CPC classification number: C07K14/79 ,  A01K67/0278 ,  A01K2217/052 ,  A01K2227/40 ,  A01K2267/01

    Abstract: PURPOSE: A transformed zebrafish massively producing human lactoferrin is provided to cheaply produce human lactoferrin and use lactoferrin as food, health food, and animal feed additive. CONSTITUTION: A transformed zebrafish which massively produces human lactoferrin is produced by transforming with a recombinant expression vector containing a human lactoferrin gene. The transformation is performed at fertilized egg of one or two cells. The transformation is performed through microinjection, electroporation, particle bombardment, sperm-mediated gene transfer, viral infection, direct muscle injection, insulator, or transposon. A method for massively producing the human lactoferrin comprises: a step of preparing the recombinant expression vector; a step of transforming the expression vector into the zebrafish fertilized egg; a step of culturing the transformed zebrafish; and a step of isolating and purifying human lactoferrin from the transformed zebrafish.

    Abstract translation: 目的：提供大规模生产人乳铁蛋白的转化斑马鱼，以廉价生产人乳铁蛋白，并使用乳铁蛋白作为食品，保健食品和动物饲料添加剂。 构成：通过用含有人乳铁蛋白基因的重组表达载体转化，产生大量产生人乳铁蛋白的转化的斑马鱼。 在1或2个细胞的受精卵中进行转化。 通过显微注射，电穿孔，粒子轰击，精子介导的基因转移，病毒感染，直接肌肉注射，绝缘体或转座子进行转化。 一种大规模生产人乳铁蛋白的方法包括：制备重组表达载体的步骤; 将表达载体转化为斑马鱼受精卵的步骤; 培养转化的斑马鱼的一个步骤; 以及从转化的斑马鱼分离和纯化人乳铁蛋白的步骤。

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