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公开(公告)号：KR1020110139363A
公开(公告)日：2011-12-29
申请号：KR1020100059400
申请日：2010-06-23
Applicant: 연세대학교 산학협력단
IPC: C12N5/0784 , C07K14/52 , A61K39/00 , A61P35/00
Abstract: PURPOSE: A method for preparing mature dendritic cells is provided to improve titer and to manufacture various dendritic cell vaccines. CONSTITUTION: A method for preparing mature dendritic cells comprises: a step of collecting immature dendritic cells; and a step of stimulating the immature dendritic cells by combination of cytokine cocktail and poly(I:C) to induce differentiation into mature dendritic cells. The cytokine cocktail contains IL-1β, IL-6, TNF-alph, PGE2 and poly(I:C). The cytokine cocktail is applied to the immature dendritic cells before application of poly(I:C). The immature dendritic cells are derived from monocytes and hematopoietic stem cell from peripheral blood, umbilical cord blood, or bone marrow. A method for manufacturing the vaccine comprises a step of co-culturing the mature dendritic cells and antigen. The dendritic cell vaccine is a cancer vaccine.
Abstract translation: 目的：提供一种制备成熟树突状细胞的方法，以提高滴度并制备各种树突状细胞疫苗。 构成：一种制备成熟树突状细胞的方法包括：收集未成熟树突状细胞的步骤; 以及通过细胞因子混合物和聚（I：C）的组合刺激未成熟树突细胞以诱导分化成成熟树突状细胞的步骤。 细胞因子混合物含有IL-1β，IL-6，TNF-α，PGE2和聚（I：C）。 在应用聚（I：C）之前，将细胞因子混合物应用于未成熟树突状细胞。 未成熟树突状细胞来自外周血，脐带血或骨髓的单核细胞和造血干细胞。 制备疫苗的方法包括共培养成熟树突状细胞和抗原的步骤。 树突状细胞疫苗是一种癌症疫苗。
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公开(公告)号：KR1020170117975A
公开(公告)日：2017-10-24
申请号：KR1020170128191
申请日：2017-09-29
Applicant: 연세대학교 산학협력단
IPC: A61K35/12 , A61K39/395 , A61K35/30 , A61K9/00
Abstract: 본발명은척수손상치료용조성물에관한것이다. 본발명에서이용되는 PSA-NCAM-양성신경전구세포는주입된조직에서의혈관신생을촉진하고염증반응을억제한다. 본발명의 PSA-NCAM-양성신경전구세포는항-PSA-NCAM-항체를이용하여간단히분리할수 있으며, 중간엽줄기세포(MSC)에비하여도우수한혈관신생및 염증억제활성을보여, 척수손상에대한효율적인치료조성물로서유용하게이용될수 있다.
Abstract translation: 本发明涉及用于治疗脊髓损伤的组合物。 用于本发明的PSA-NCAM阳性神经祖细胞促进注射组织中的血管生成并抑制炎症反应。 本发明的PSA-NCAM-阳性神经祖细胞可以容易地分离，其中，使用所述NCAM- -psa抗体，它表现出了间充质干细胞（MSC）EBI hayeodo优良的血管生成和炎症的抑制，对脊髓损伤 可以有效地用作有效的治疗组合物。
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公开(公告)号：KR1020160135885A
公开(公告)日：2016-11-29
申请号：KR1020150068860
申请日：2015-05-18
Applicant: 가톨릭관동대학교기술지주 주식회사 , 연세대학교 산학협력단
IPC: A61K35/28 , A61K35/30 , A61K35/407 , A61K47/48
Abstract: 본발명은줄기세포유래미세소포체를유효성분으로포함하는심혈관질환예방또는치료용조성물에관한것이다.
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14.发明授权PSA-NCAM-양성 신경전구세포를 유효성분으로 포함하는 허혈성 질환 또는 신경염증 질환 치료용 조성물 有权PSA-NCAM-用于治疗缺血性疾病或神经炎症疾病的组合物，其包含作为活性成分的PSA-NCAM阳性神经前体细胞
公开(公告)号：KR101675123B1
公开(公告)日：2016-11-14
申请号：KR1020140080009
申请日：2014-06-27
Applicant: 연세대학교 산학협력단
IPC: A61K35/12 , A61K39/395 , A61K35/30 , A61K9/00
CPC classification number: A61K35/30 , A61K35/12 , A61K39/395 , C12N5/0623 , C12N2500/25 , C12N2500/90 , C12N2501/115 , C12N2506/02
Abstract: 본발명은허혈성질환또는신경염증질환치료용조성물에관한것이다. 본발명에서이용되는 PSA-NCAM-양성신경전구세포는주입된조직에서의혈관신생을촉진하고염증반응을억제한다. 본발명의 PSA-NCAM-양성신경전구세포는항-PSA-NCAM-항체를이용하여간단히분리할수 있으며, 중간엽줄기세포(MSC)에비하여도우수한혈관신생및 염증억제활성을보여, 혈관손상으로인한허혈성질환및 염증으로인한신경손상질환에대한효율적인치료조성물로서유용하게이용될수 있다.
Abstract translation: 本发明提供了用于治疗缺血性疾病或神经源性炎症的组合物。 用于本发明的PSA-NCAM阳性神经祖细胞促进注射组织中的血管发生并抑制炎性反应。 可以通过使用抗PSA-NCAM-抗体简单地分离PSA-NCAM阳性神经祖细胞，并且与间充质干细胞相比表现出优异的血管生成和抗炎活性，因此可用作有效治疗的组合物 由血管损伤引起的缺血性疾病和由炎症引起的神经损伤疾病。 此外，本发明的神经祖细胞的分泌物减少了缺血性损伤部位，并且能够恢复神经功能，因此可以用作治疗缺血性疾病和退行性神经系统疾病如神经损伤疾病引起的药物 通过炎症。
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公开(公告)号：KR1020130115562A
公开(公告)日：2013-10-22
申请号：KR1020120037954
申请日：2012-04-12
Applicant: 연세대학교 산학협력단
IPC: C12M3/00 , C12N5/074 , C12M1/42 , C07D207/00
CPC classification number: C12N5/0068 , C07D495/04 , C07D517/04 , C12M23/20 , C12M25/06 , C12M33/00 , C12N2529/10 , C12N2533/30 , C12N2535/10 , C12N2539/10
Abstract: PURPOSE: A method for selectively detaching, patterning, and harvesting cells by near infrared rays is provided to selectively detach cells without enzyme treatment using a conductive polymer or a metal oxide film as a support for cell culture. CONSTITUTION: A cell culture container has a cell culture region containing a conductive polymer or a metal oxide film with an absorbance within a near infrared ray region. The conductive polymer is a polymer or a copolymer containing monomers selected among a compound of chemical formula 1 and aniline. The cultured cells are detached by being irradiated by near infrared rays. A patterned substrate for cell culture comprises: a substrate; and the cell culture region containing the conductive polymers or the metal oxide film with the absorbance within the near infrared ray region.
Abstract translation: 目的：提供一种用于通过近红外线选择性分离，图案化和收获细胞的方法，以使用导电聚合物或金属氧化物膜作为细胞培养的支持来选择性地分离细胞而不进行酶处理。 构成：细胞培养容器具有含有导电性聚合物或金属氧化物膜的细胞培养区域，在近红外线区域内具有吸光度。 导电聚合物是含有选自化学式1化合物和苯胺的单体的聚合物或共聚物。 培养的细胞被近红外线照射分离。 用于细胞培养的图案化底物包括：底物; 以及含有导电性聚合物或金属氧化物膜的细胞培养区，其吸光度在近红外线区域内。
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Patent Agency Ranking
公开(公告)号：KR101038699B1
公开(公告)日：2011-06-02
申请号：KR1020090010077
申请日：2009-02-09
Applicant: 연세대학교 산학협력단
Abstract: 본 발명은 형광 화합물을 포함하는 생체 친화성 필름 조성물, 생체 친화성 산화막 필름 및 그의 제조 방법, 이를 이용한 생체물질의 부착 방법 및 세포의 증식 또는 분화 방법을 제공한다. 본 발명에 따른 생체 친화성 필름 조성물을 이용하여 제작된 생체 친화성 산화막 필름은 산화가 일어난 부위에서만 선택적으로 표면 특성이 친수성으로 변화하여 생체 친화성을 나타낸다. 이러한 특성을 이용하여 본 발명의 생체 친화성 산화막 필름 상에서 세포를 배양할 경우, 일반 세포 분화 용기에 비해 세포의 부착, 증식, 또는 분화를 효과적으로 수행할 수 있다. 또한, 본 발명의 생체 친화성 산화막 필름을 부분적으로 산화시켜 형광 패턴을 갖도록 제작할 경우, 패턴의 크기와 모양을 조절함으로써 세포나 단백질을 포함한 생체물질의 부착을 조절할 수 있으며, 특히, 형광 패턴의 크기와 모양을 조절함으로써 줄기 세포의 증식과 분화를 촉진할 수 있으므로, 바이오 엔지니어링 분야에 효과적으로 응용될 수 있다.
형광 화합물, 산화, 생체 물질 부착, 패턴, 세포 배양, 증식, 분화-
公开(公告)号：KR100806677B1
公开(公告)日：2008-02-26
申请号：KR1020070019968
申请日：2007-02-28
Applicant: 연세대학교 산학협력단
Abstract: An artificial trachea having a bi-layered polyurethane scaffold with a polyethylene glycol-grafted surface and a manufacturing method thereof are provided to prevent adhesion of foreign substances and formation of eschar until a normal respiratory epithelial cell grows from surroundings. An artificial trachea having a bi-layered polyurethane scaffold with a polyethylene glycol-grafted surface includes: porous polyurethane matrix having one surface grafted by polyethylene glycol; and a dense polyurethane film. The surface grafting is formed by ozonation. The polyurethane matrix is formed by introducing peroxide on the surface of a support and connecting polyethylene glycol thereon. The polyurethane matrix has plural pores with a size of 150 -250 micrometers.
Abstract translation: 提供具有聚乙二醇接枝表面的双层聚氨酯支架的人工气管及其制造方法，以防止外来物质的粘附和焦痂的形成，直到正常的呼吸上皮细胞从周围生长。 具有聚乙二醇接枝表面的双层聚氨酯支架的人造气管包括：具有由聚乙二醇接枝的一个表面的多孔聚氨酯基质; 和聚氨酯薄膜。 表面接枝由臭氧化形成。 通过在载体的表面上引入过氧化物并在其上连接聚乙二醇来形成聚氨酯基质。 聚氨酯基质具有多个尺寸为150-250微米的孔。
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公开(公告)号：KR101818560B1
公开(公告)日：2018-01-16
申请号：KR1020150068860
申请日：2015-05-18
Applicant: 가톨릭관동대학교기술지주 주식회사 , 연세대학교 산학협력단
IPC: A61K35/28 , A61K35/30 , A61K35/407 , A61K47/48
Abstract: 본발명은줄기세포유래미세소포체를유효성분으로포함하는부정맥예방또는치료용조성물에관한것이다.
Abstract translation: 本发明涉及用于预防或治疗心律失常的组合物，其包含源自干细胞的微囊泡作为活性成分。
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公开(公告)号：KR1020120124596A
公开(公告)日：2012-11-14
申请号：KR1020110042329
申请日：2011-05-04
Applicant: 연세대학교 산학협력단
IPC: G01N33/53 , C12N5/0735 , C12N5/079 , G01N33/52
Abstract: PURPOSE: A method for removing undifferentiated pluripotent stem cells and a cell composition are provided to effectively and simply remove the undifferentiated pluripotent stem cells with low contamination rate. CONSTITUTION: A method for removing undifferentiated pluripotent stem cells comprise: a step of preparing a cell sample containing undifferentiated pluripotent stem cells and differentiated cells; a step of contacting SSEA-3(stage-specific embryonic antigen-3) antibody and TRA(tumor rejection antigen)-1-60 antibody to the cell sample; and a step of sorting cells conjugated with SSEA-3 antibody and TRA-1-60 antibody. The undifferentiated pluripotent stem cells are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells or iPSCs(induced pluripotent stem cells). The undifferentiated pluripotent stem cells are derived from human, horse, cow, goat, sheep, dog, cat, mouse, rat, or birds.
Abstract translation: 目的：提供一种去除未分化多能干细胞和细胞组成的方法，以有效和简单地以低污染率除去未分化的多能干细胞。 构成：去除未分化多能干细胞的方法包括：制备含有未分化多能干细胞和分化细胞的细胞样品的步骤; 将SSEA-3（阶段特异性胚胎抗原-3）抗体和TRA（肿瘤排斥抗原）-1-60抗体与细胞样品接触的步骤; 以及分选与SSEA-3抗体和TRA-1-60抗体缀合的细胞的步骤。 未分化的多能干细胞是胚胎干细胞，胚胎生殖细胞，胚胎癌细胞或iPSC（诱导多能干细胞）。 未分化的多能干细胞衍生自人，马，牛，山羊，绵羊，狗，猫，小鼠，大鼠或鸟。
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公开(公告)号：KR1020100090869A
公开(公告)日：2010-08-18
申请号：KR1020090010077
申请日：2009-02-09
Applicant: 연세대학교 산학협력단
Abstract: PURPOSE: A bio-affinity oxide layer film and a manufacturing method thereof are provided to improve the bio-affinity by selectively charging the surface property of the film into the hydrophilic property. CONSTITUTION: A bio-affinity oxide layer film composition contains a fluorescent compound. The fluorescent compound is a compound selected from the group consisting of a polymer including a compound marked with chemical formula 1 as a recurring unit, a polymer including a compound marked with chemical formula 2 as a recurring unit, and a polymer including a compound marked with chemical formula 3 as a recurring unit. A manufacturing method of the bio-affinity oxide layer film comprises the following steps: spreading the composition on a substrate; and oxidizing the composition.
Abstract translation: 目的：提供一种生物亲和氧化物膜及其制造方法，通过选择性地将膜的表面性质填充到亲水性中来提高生物亲和力。 构成：生物亲和氧化物膜组合物含有荧光化合物。 荧光化合物是选自包含以化学式1标记的化合物作为重复单元的聚合物的聚合物，包含以化学式2表示的重复单元的化合物的聚合物，以及包含标记有 化学式3为重复单元。 生物亲和氧化物层膜的制造方法包括以下步骤：将组合物铺展在基材上; 并氧化组合物。
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