Abstract:
Cell culture system and method of a biomimetic bioreactor are disclosed. The cell culture system which cultures joint cells comprises: a joint part which comprises a first joint part in which a first scaffold conjugated on the surface and a second joint part in which a second scaffold is conjugated on the surface facing the first joint part; a culture chamber which receives the joint part and is able to culture cells on a first artificial support and a second artificial support; and a storage unit which is connected with the culture chamber by a connection tube supplies a culture medium for culturing the cells to the culture chamber. The cell culture system is able to effectively culture chondrocytes by realizing a culture medium circulation method, a joint movement structure, a scaffold insertion structure, and a chondrocyte culture to make an optimal culture environment in an in vitro culture chamber in which chondrocytes are cultured.
Abstract:
PURPOSE: A porous structure manufacturing method is provided to quickly and efficiently manufacture a porous structure having uniform pores. CONSTITUTION: A porous structure manufacturing method comprises: a step of forming a wire network molding(300) by inserting a wire network(200) into a porous mold(100) in which pores are formed in the surface thereof; a step of filling the wire network molding with materials having a porous structure; a step of removing the wire; and a step of removing the porous mold.
Abstract:
An operation system for cell culture of a biomimetic bioreactor is disclosed. The operation system for culturing joint cells comprises: a joint part with a first joint part in which a first scaffold is conjugated on the surface thereof and a second joint part facing the first joint part, in which a second scaffold is conjugated on the surface thereof; a culture chamber which receives the joint part and is used for culturing cells on the first scaffold and the second scaffold; a first rod which is extended and coupled to the first joint part; a second rod which is extended and coupled to the second joint part facing the first joint part; and a bending angle adjusting unit which adjusts the angle of the first and second rods. The operation system for cell culture is able to effectively culture chondrocytes by forming a joint structure for a culture medium circulation method, a joint movement structure, a scaffold insertion structure, and a chondrocyte culture to make an optimal culture condition in an in vitro culture chamber in which chondrocytes are cultured.
Abstract:
PURPOSE: A double scaffold for co-culturing bone cell and cartilage cell is provided to regenerate efficient bone and cartilage double tissue. CONSTITUTION: A double scaffold for co-culturing bone cell and cartilage cell comprises: a scaffold(52) for culturing bone cell; and a scaffold(51) for culturing cartilage cell. The scaffold for culturing bone cell and scaffold for culturing cartilage cell are laminated in a dual structure. Each scaffold is formed of several micro channel(51',52') penetrating from one side to the other side.