公开(公告)号：KR101980576B1

公开(公告)日：2019-05-22

申请号：KR1020170086082

申请日：2017-07-06

Applicant: 충남대학교산학협력단

Inventor： 이혜미 ,  조은경 ,  구본정

IPC: G01N33/68 ,  C12Q1/68

公开(公告)号：KR101971104B1

公开(公告)日：2019-04-22

申请号：KR1020180000721

申请日：2018-01-03

Applicant: 충남대학교산학협력단

Inventor： 이혜미 ,  조은경 ,  정재욱

IPC: C12Q1/68 ,  G01N33/68

公开(公告)号：KR20180044667A

公开(公告)日：2018-05-03

申请号：KR20160138467

申请日：2016-10-24

Applicant: 충남대학교산학협력단

Inventor： 조은경 ,  이혜미 ,  김진경

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/178

Abstract: 본발명은마이크로 RNA를마커로이용한자가포식조절제의스크리닝방법에관한것으로, 보다상세하게는 (A) miRNA-144를발현하는세포또는조직에시험물질을처리하는단계; 및 (B) 상기시험물질이처리된세포또는조직에서 miRNA-144의발현을측정하는단계; 및 (C) 상기 (B) 단계에서측정된 miRNA-144의발현수준이시험물질을처리하지않은대조군에비해변화된경우자가포식조절제로판정하는단계;를포함하는것을특징으로하는자가포식조절제의스크리닝방법에관한것이다.

公开(公告)号：KR1020130116797A

公开(公告)日：2013-10-24

申请号：KR1020130027015

申请日：2013-03-14

Applicant: 충남대학교산학협력단

Inventor： 조은경 ,  이혜미 ,  구본정

IPC: G01N33/53 ,  C12Q1/02 ,  C12N5/0786

Abstract: PURPOSE: A reagent for diagnosis and follow-up study on type 2 diabetes and a test method including a measurement of IL-1 beta are provided to accurately diagnose type 2 diabetes with convenience and comfortableness. CONSTITUTION: A novel method for diagnosing type 2 diabetes comprises the steps of: inducing macrophage from monocytes collected from blood of a person who is suspected to have diabetes and culturing the macrophage; measuring the concentration of active IL-1 beta secreted from the cultured macrophage; and determining type 2 diabetes when the concentration of active IL-1 beta is over a predetermined level. A method for determining treatment effects on type 2 diabetes comprises the steps of: inducing macrophage from monocytes collected from a patient's blood with a type 2 diabetes during treatment and culturing the macrophage; measuring the concentration of active IL-1 beta secreted from the cultured macrophage; and determining that a treatment has an effect on the patient when the concentration of active IL-1 beta is less than a predetermined level. [Reference numerals] (A) Patient with type 2 diabete before meformin treatment; (B) Patient with type 2 diabete after meformin treatment; (H) Ordinary person

Abstract translation: 目的：提供一种用于2型糖尿病诊断和随访研究的试剂和包括测量IL-1β的测试方法，以便于方便和舒适地准确诊断2型糖尿病。 构成：用于诊断2型糖尿病的新方法包括以下步骤：从怀疑患有糖尿病并培养巨噬细胞的人的血液中收集的单核细胞诱导巨噬细胞; 测量从培养的巨噬细胞分泌的活性IL-1β的浓度; 并且当活性IL-1β的浓度超过预定水平时确定2型糖尿病。 用于确定对2型糖尿病的治疗效果的方法包括以下步骤：在处理和培养巨噬细胞期间从患有血清的2型糖尿病中收集的单核细胞诱导巨噬细胞; 测量从培养的巨噬细胞分泌的活性IL-1β的浓度; 并且当活性IL-1β的浓度小于预定水平时，确定治疗对患者有影响。 （附图标记）（A）甲泼尼龙治疗前2型糖尿病患者; （B）甲吡啶治疗后2型糖尿病患者; （H）普通人