公开(公告)号：KR1020090064935A

公开(公告)日：2009-06-22

申请号：KR1020070132320

申请日：2007-12-17

Applicant: 한국전자통신연구원

Inventor： 신동호 ,  김용준 ,  정민숙 ,  김상희 ,  김혜윤 ,  정문연 ,  박선희

IPC: G01N35/00

CPC classification number: G01N33/491 ,  Y10T29/49826

Abstract: A filter chip and a manufacture method thereof are provided to obtain maximum amount of blood plasma by increasing capillary force of a fluid inlet and to remove blood cells and minute particles, which obstruct diagnosis or analysis. A filter chip comprises: a filter(130); a lower plate structure(110) with a groove(111) formed therein in order to settle the filter; an upper plate structure(120) covering the upper area of the lower plate structure; a fluid inlet(140) formed in one region of the upper plate structure in order to inject fluids; and a fluid outlet(150) formed lower than the fluid inlet in order to flow the fluid out. The width of the filter and groove becomes narrow from the fluid inlet side to the fluid outlet side so as to induce rapid capillary force as the fluid passes through the filter.

Abstract translation: 提供了一种过滤芯片及其制造方法，其通过增加流体入口的毛细管力而获得最大量的血浆，并除去妨碍诊断或分析的血细胞和微小颗粒。 滤芯包括：过滤器（130）; 下板结构（110），其中形成有凹槽（111），以便沉积过滤器; 覆盖所述下板结构的上部区域的上板结构（120）; 形成在上板结构的一个区域中以便注入流体的流体入口（140） 以及形成在流体入口下方的流体出口（150），以使流体流出。 过滤器和槽的宽度从流体入口侧到流体出口侧变窄，以便当流体通过过滤器时引起快速的毛细作用力。

公开(公告)号：KR1020130064461A

公开(公告)日：2013-06-18

申请号：KR1020110131088

申请日：2011-12-08

Applicant: 한국전자통신연구원

Inventor： 박정원 ,  정민숙 ,  정문연

IPC: G01N35/08 ,  G01N33/49 ,  G01N1/28 ,  G01N33/574

Abstract: PURPOSE: A device for separating tumor cells from blood is provided to minimize damage to collected tumor cells by using a microstructure which controls pressures. CONSTITUTION: A device for separating tumor cells from blood includes inlets(102i), a fluid channel(110), and outlets(102o). Blood is injected through the inlets. The fluid channel is equipped with at least one loop-shaped microstructure(120). The blood is transferred through the fluid channel, and the fluid channel collects tumor cells from the blood. Blood without the tumor cells is discharged through the outlet. The microstructure includes a body(B), an introducing unit, a discharging unit, and a plurality of inlets, and a plurality of outlets. The body is composed of two parallel lines. The introducing unit and the discharging unit are hemi-circular shapes and are adjacent to the inlet and outlet by being in contact with both sides of the body. The inlets are larger than the tumor cells and are formed at 45 degrees of the center of the hemi-circular shape. The outlets are smaller than the tumor cells and larger than blood corpuscle. The outlets are formed over the entire discharging unit.

Abstract translation: 目的：提供用于从血液中分离肿瘤细胞的装置，以通过使用控制压力的微结构来最小化对收集的肿瘤细胞的损伤。 构成：用于从血液中分离肿瘤细胞的装置包括入口（102i），流体通道（110）和出口（102o）。 血液通过入口注射。 流体通道配备有至少一个环形微结构（120）。 血液通过流体通道传输，流体通道从血液中收集肿瘤细胞。 没有肿瘤细胞的血液通过出口排出。 微结构包括主体（B），引入单元，排出单元和多个入口以及多个出口。 身体由两条平行线组成。 引入单元和排出单元是半圆形的并且通过与主体的两侧接触而与入口和出口相邻。 入口大于肿瘤细胞，并形成在半圆形中心的45度处。 出口小于肿瘤细胞，大于血小板。 出口形成在整个排放单元上。

公开(公告)号：KR101189129B1

公开(公告)日：2012-10-10

申请号：KR1020080131856

申请日：2008-12-23

Applicant: 한국전자통신연구원

Inventor： 신동호 ,  정민숙 ,  송규호 ,  김혜윤 ,  전주현 ,  정문연 ,  박선희

IPC: G01N30/26 ,  G01N33/563 ,  G01N33/553

CPC classification number: B01L3/50273 ,  B01L9/527 ,  B01L2400/0457 ,  G01N35/08 ,  Y10T436/11

Abstract: 본 발명은 미세유체 소자의 유체 흐름 조절 방법 및 미세유체분석장치에 관한 것으로, 이 방법은 수평에 대한 미세유체소자의 기울기를 조절함으로써, 미세유체소자 내에서 간단하고 정밀하게 유체의 흐름을 제어할 수 있다. 이로써 유체의 이송을 완벽하게 제어할 수 있으며, 적은 양의 시료만으로 검사를 진행할 수 있다. 이 방법은 사용자에 의해 수동으로 진행될 수 있어 전력을 필요로 하지 않아 경제적이며, 간단하다. 또한 이 미세유체 분석 장치는 수평에 대해 상기 미세유체소자 수용부의 기울기를 유발하기 위한 기울기 작동수단과 상기 기울기 작동수단의 동작 상태를 제어하기 위한 기울기 제어부를 구비함으로써, 간단하고 정밀하게 유체의 흐름을 제어하여 유체를 정확하게 분석할 수 있다.
미세유체소자, 미세유체분석장치, 기울기, 중력, 모세관력

公开(公告)号：KR1020110059104A

公开(公告)日：2011-06-02

申请号：KR1020090115740

申请日：2009-11-27

Applicant: 한국전자통신연구원

Inventor： 정민숙

IPC: G01N33/53 ,  G01N33/543

CPC classification number: G01N33/54393 ,  G01N2333/4712

Abstract: PURPOSE: A method for detecting proteins using a microfluidic chip is provided to minimize the interference of an immune response and to maximize the immune response. CONSTITUTION: A microfluidic chip(100) comprises: a filter unit(110) having a filter in which an anti-immunoglobulin antibody is fixed; a first reaction unit(120) for conjugating a detection antibody with a protein to be detected from blood; and a second reaction and detection unit for detecting the protein concentration in fluorescence intensity by capturing the protein with a capture antibody. The filter is a membrane filter which is able to isolate blood cells and plasma. The protein to be detected is cTnI(cardiac troponin I).

Abstract translation: 目的：提供使用微流控芯片检测蛋白质的方法，以最大限度地减少免疫反应的干扰并使免疫应答最大化。 构成：微流体芯片（100）包括：具有固定抗体免疫球蛋白抗体的过滤器的过滤器单元（110）; 第一反应单元（120），用于将检测抗体与待检测的蛋白质与血液共轭; 以及第二反应和检测单元，用于通过用捕获抗体捕获蛋白质来检测荧光强度中的蛋白质浓度。 过滤器是能够分离血细胞和血浆的膜过滤器。 待检测的蛋白质是cTnI（心肌肌钙蛋白I）。

公开(公告)号：KR1020090059448A

公开(公告)日：2009-06-11

申请号：KR1020070126314

申请日：2007-12-06

Applicant: 한국전자통신연구원

Inventor： 최요한 ,  정광효 ,  이대식 ,  김상희 ,  정민숙 ,  전치훈 ,  정문연 ,  박선희

IPC: C12M3/00 ,  C12M1/00

CPC classification number: C12M23/20 ,  B01L3/5027 ,  B01L7/00 ,  C12M23/16 ,  C12M41/12 ,  Y10T29/49124

Abstract: A portable small cell incubator is provided to supply gas which is necessary in culturing animal cell and regulate the temperature of culture medium. A portable small cell incubator contains an upper plate(100), lower plate(300) and middle thin film(200). The upper plate contains a minute heat wire(110) and medium storage space(130) by PDMS(polydimethylsiloxane). The lower plate contains a storage space(330) of liquid and solid and gas movement channel. A method for manufacturing the incubator comprises: a step of depositing a metal on the lower plate; a step of forming minute heat wire; a step of attaching a PDMS(polydimethylsiloxane) film on the upper side of lower plate; a step of attaching PDMS thin film having gas permeability on the upper side of gas movement channel; and a step of attaching the upper plate.

Abstract translation: 提供便携式小细胞培养器来提供培养动物细胞所必需的气体并调节培养基的温度。 便携式小电池培养箱包含上板（100），下板（300）和中间薄膜（200）。 上板由PDMS（聚二甲基硅氧烷）包含微小的热丝（110）和介质储存空间（130）。 下板包含液体和固体和气体运动通道的储存空间（330）。 一种用于制造所述培养箱的方法，包括：在所述下板上沉积金属的步骤; 形成分热丝的步骤; 在下板的上侧附着PDMS（聚二甲基硅氧烷）膜的步骤; 在气体流动通道的上侧附着具有透气性的PDMS薄膜的步骤; 以及附接上板的步骤。

公开(公告)号：KR101135084B1

公开(公告)日：2012-04-16

申请号：KR1020080131860

申请日：2008-12-23

Applicant: 한국전자통신연구원

Inventor： 신동호 ,  정민숙 ,  송규호 ,  김혜윤 ,  전주현 ,  정문연 ,  박선희

IPC: G01N30/26 ,  G01N33/563 ,  G01N33/553

CPC classification number: G01N21/645 ,  B01L3/50273 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/043 ,  B01L2400/0475 ,  B01L2400/0688

Abstract: 본 발명은 미세 유체 소자 및 미세 유체 분석 장치에 관한 것으로, 이 소자는 양측에 형성된 가이드들과, 가이드들 사이에 형성된 유로를 포함하는 하판과, 하판 상에서 가이드들을 따라 이동하되 유로의 길이보다 짧은 길이를 가지는 이동상판을 포함하며, 이동상판의 위치를 조절함으로써 간단하고 정밀하게 유체의 흐름을 제어할 수 있다. 이로써 감지부나 반응부에서 유체의 반응이 충분히 이루어지도록 조절할 수 있어, 유체의 적은 양으로도 효과적인 반응 및 감지를 구현할 수 있으며, 감도를 향상시킬 수 있다. 또한 감도향상으로 반응에 참여하지 않은 물질을 제거하는 세척(washing) 과정을 생략할 수 있다. 또한 상기 이동 상판은 손가락으로 수동으로 조절할 수도 있다.
미세유체소자, 분석장치, 랩온어칩, 표면장력

公开(公告)号：KR1020100073241A

公开(公告)日：2010-07-01

申请号：KR1020080131860

申请日：2008-12-23

Applicant: 한국전자통신연구원

Inventor： 신동호 ,  정민숙 ,  송규호 ,  김혜윤 ,  전주현 ,  정문연 ,  박선희

IPC: G01N30/26 ,  G01N33/563 ,  G01N33/553

CPC classification number: G01N21/645 ,  B01L3/50273 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/043 ,  B01L2400/0475 ,  B01L2400/0688

Abstract: PURPOSE: A micro-fluidic device and a micro-fluid analysis apparatus are provided to secure the enough reaction of fluid at a sensor or a reactor by simply controlling the flow of the fluid, and to adjust the reaction time of the fluid. CONSTITUTION: A micro-fluidic device comprises the following: a storing chamber(5) inserted with fluid; guides formed along to both edges of the storing chamber; a lower plate(20) including a channel(24) connected with the storing chamber, while being located among the guides; an upper plate(40) including a fluid inlet(12); sensors(8,9) detecting specific materials from the fluid; and a transfer plate moving along to the guides. The fluid flows along to the transfer plate while contacting the lower side of the transfer plate.

Abstract translation: 目的：提供微流体装置和微流体分析装置，通过简单地控制流体的流动并调节流体的反应时间来确保流体在传感器或反应器处的足够的反应。 构成：微流体装置包括：插入有流体的储存室（5）; 引导件沿着存储室的两个边缘形成; 下部板（20），其包括与所述存储室连接的通道（24），同时位于所述引导件中; 包括流体入口（12）的上板（40）; 传感器（8,9）检测来自流体的特定材料; 以及沿着引导件移动的转印板。 流体在接触转印板的下侧的同时流向转印板。

公开(公告)号：KR1020100061605A

公开(公告)日：2010-06-08

申请号：KR1020080120190

申请日：2008-11-29

Applicant: 한국전자통신연구원

Inventor： 정민숙 ,  정문연 ,  박선희

IPC: C12N5/071 ,  C12N5/02 ,  C12N5/00

CPC classification number: C12N5/0655 ,  A61K35/12 ,  C12N2525/00

Abstract: PURPOSE: A method for differentiating from mesenchymal stem cells to catilage and a composition for treating cartilage injury are provided to achieve cartilage differentiation with low price. CONSTITUTION: A method for differentiating from mesenchymal stem cells to cartilage comprises: a step of culturing mesenchymal stem cells in a monolayer; a step of culturing in a three-dimension; and a step of applying centrifugal force to differentiate to chondrogenic cells. The mesenchymal stem cells are human mesenchymal stem cells and derived from human embroy, adult tissue, or bone marrow. A composition for treating cartilage injury contains the chondrogenic cells.

Abstract translation: 目的：提供用于区分间充质干细胞与灭活的方法和用于治疗软骨损伤的组合物，以实现低价格的软骨分化。 构成：从间充质干细胞与软骨分化的方法包括：在单层培养间充质干细胞的步骤; 三维培养的步骤; 以及施加离心力以分化成软骨形成细胞的步骤。 间充质干细胞是人类间充质干细胞，源于人体，成人组织或骨髓。 用于治疗软骨损伤的组合物含有软骨形成细胞。

公开(公告)号：KR1020090039130A

公开(公告)日：2009-04-22

申请号：KR1020070104589

申请日：2007-10-17

Applicant: 한국전자통신연구원

Inventor： 정문연 ,  신동호 ,  정민숙 ,  김상희 ,  김혜윤 ,  박선희

IPC: G01N33/49 ,  G01N33/48 ,  G01N33/00 ,  G01N35/00

CPC classification number: G01N33/49

Abstract: A capillary force Lab-On-A-Chip is provided to control flow of microfluid by freely changing the capillary force of Lab-On-A-Chip and improve the function of controlling reaction time between a fluid sample to analyze and reagent. A capillary force Lab-On-A-Chip comprises: the first area in which the first substrate(110) and the second substrate(120) are bonded together; the second area in which the first substrate and the second substrate are not bonded; gap control member, which is equipped at the end of the second area which faces against the boundary between the first area and second area, controlling a capillary force by controlling the gap between the first substrate and the second substrate. A method for driving the Lab-On-A-Chip comprises: a step of preparing Lab-On-A-Chip containing the first area and the second area and a step of controlling flow of fluid inside the second area by controlling the gap between the first substrate and the second substrate of the end of the second area corresponding to the boundary of the first area and the second area.

Abstract translation: 提供毛细管力实验芯片，通过自由地改变实验芯片上的毛细管力来控制微流体的流动，提高控制流体样品分析和试剂之间的反应时间的功能。 毛细管力实验芯片A芯片包括：第一衬底（110）和第二衬底（120）结合在一起的第一区域; 第一基板和第二基板未接合的第二区域; 间隙控制构件，其设置在面对第一区域和第二区域之间的边界的第二区域的端部，通过控制第一基板和第二基板之间的间隙来控制毛细管力。 一种用于驱动实验片上A芯片的方法包括：制备包含第一区域和第二区域的实验室芯片的步骤，以及通过控制第二区域内的间隙来控制第二区域内的流体流动的步骤 第二区域的端部的第一基板和第二基板对应于第一区域和第二区域的边界。