公开(公告)号：KR1020140025816A

公开(公告)日：2014-03-05

申请号：KR1020120091973

申请日：2012-08-22

Applicant: 한국화학연구원

Inventor： 김진석 ,  김슬기 ,  고은혜

IPC: C12N1/12 ,  C12N1/04

CPC classification number: C12N1/12

Abstract: The present invention provides economical and efficient methods related to mass production, high quality biomass, harvesting, storage, and preprocess of hydrodictyon algae, which have never been reported about mass production among freshwater algae. The methods comprise: inducing outstanding seeds; optimally managing culturing conditions for increasing production and highly containing effective materials; harvesting/dehydrating the grew hydrodictyon algae; and drying/storing/preprocessing the harvested biomass. The hydrodictyon algae cultured by the present invention: are non-edible algae so that skyrocket of food price and ethical problems will not be generated when industrial demand of the biomass gets higher; can be produced at low costs using waste sources (waste water and waste CO2) so that resources can be stably provided; and have increased contents of effective ingredients (carbohydrates, protein, and the likes) and are easily saccharified, thereby being useful as an active ingredient for producing biochemical products. [Reference numerals] (AA) Step of obtaining and maintaining outstanding hydrodictyon algae seed; (BB) Step of mass producing high quality hydrodictyon algae biomass; (CC) Step of harvesting and dehydrating hydrodictyon algae biomass; (DD) Step of drying and storing hydrodictyon algae biomass

Abstract translation: 本发明提供了关于大规模生产，高质量生物质，收获，储存和预处理的水文藻类的经济和有效的方法，从未报道过关于淡水藻类的大规模生产。 方法包括：诱发优秀种子; 最佳地管理增加生产和高含量有效材料的培养条件; 收获/脱水生长的水貂藻; 并干燥/储存/预处理收获的生物量。 本发明培养的水貂藻是非食用藻类，当生物量的工业需求增加时，不会产生食物价格和伦理问题的激增; 可以使用废物（废水和废二氧化碳）以低成本生产，以便能够稳定地提供资源; 并增加有效成分（碳水化合物，蛋白质等）的含量，容易糖化，从而可用作生物化学产品的活性成分。 （附图标记）（AA）获得和保持优良的水貂藻类种子的步骤; （BB）大规模生产高品质海藻藻类生物质的步骤; （CC）水解藻类生物质的收获和脱水步骤; （DD）干燥和储存海洋藻类生物质的步骤

公开(公告)号：KR101361146B1

公开(公告)日：2014-02-13

申请号：KR1020120029656

申请日：2012-03-23

Applicant: 한국화학연구원

Inventor： 김진석 ,  김진철 ,  김슬기 ,  뉴엔마이꾸옹 ,  고은혜

IPC: C12P7/06 ,  C12N1/12 ,  C12R1/89

CPC classification number: A01G33/00 ,  C12N1/12 ,  C12P7/06 ,  Y02A40/88 ,  Y02E50/17

Abstract: 본 발명은 그물말 과(
Family Hydrodictyaceae ) 조류 바이오매스 및 이의 당화액으로부터 바이오에탄올을 제조하는 방법에 관한 것으로, 보다 상세하게는 (a) 그물말 과 조류를 당화시켜 단당류를 포함하는 당화액을 수득하는 단계; 및 (b) 상기 당화액을 탄소원으로 이용하여 미생물을 배양하여 바이오에탄올을 제조하는 단계를 포함하는 그물말 과(
Family Hydrodictyaceae ) 조류로부터 바이오에탄올의 제조방법을 제공한다. 본 발명에 따르면, 그물말 과 조류로부터의 화학물질 생산은 옥수수 및 밀과 같은 식용자원이 아닌 비식용자원인 조류(algae)를 활용하기 때문에 바이오매스의 산업적 수요가 높아졌을 때 식품 가격 폭등 및 윤리적 문제의 발생 소지가 없고, 안정적으로 자원의 공급이 가능하고, 본 발명의 제조방법에 의해 제조된 바이오에탄올은 수송용 연료로 제공되거나 여러 가지 화학제품 생산의 원료물질로 제공되어 석유대체 화학물질 생산에 기여할 것이다.

公开(公告)号：KR1020130107686A

公开(公告)日：2013-10-02

申请号：KR1020120029656

申请日：2012-03-23

Applicant: 한국화학연구원

Inventor： 김진석 ,  김진철 ,  김슬기 ,  뉴엔마이꾸옹 ,  고은혜

IPC: C12P7/06 ,  C12N1/12 ,  C12R1/89

CPC classification number: A01G33/00 ,  C12N1/12 ,  C12P7/06 ,  Y02A40/88 ,  Y02E50/17

Abstract: PURPOSE: A method for producing bioethanol from Hydrodictyon family algae is provided to stably supply resources without moral problems and a sharp rise in food prices using algae when industrial demands for biomass increase. CONSTITUTION: A method for producing bioethanol from Hydrodictyon family algae comprises the steps of: saccharifying Hydrodictyon family algae and preparing a saccharified liquid containing monosaccharides; and culturing microorganisms using the saccharified liquid as a carbon source and producing bioethanol. The Hydrodictyon family algae are selected among Hydrodictyon sp., Pediastrum sp., and Sorastrum sp. The saccharification is performed using a hydrolytic catalyst, a polysaccharide decomposing enzyme, or a mixture thereof. [Reference numerals] (AA) Consecutive direct saccharifying/ fermenting process; (BB) Hydrodictyon family algae and algae biomass; (CC,GG,NN) Saccharification enzyme; (DD) Saccharifying; (EE) 40-600°C, 1 day; (FF) Algae biomass; (HH,OO) Ethanol producing strain; (II) Fermenting; (JJ,QQ) 30-40°C, 3 days; (KK,RR) Bio ethanol; (LL) Simultaneous direct saccharifying/ fermenting process; (MM) Hydrodictyon family algae and algae biomass (injected one time or two or more time); (PP) Saccharifying/fermenting

Abstract translation: 目的：提供一种从Hydrodictyon家族藻类生产生物乙醇的方法，以便在生物质工业需求增加时，稳定地供应没有道德问题的资源和使用藻类的食品价格大幅上涨。 构成：从Hydrodictyon家族藻类生产生物乙醇的方法包括以下步骤：糖化Hydrodictyon家族藻类并制备含有单糖的糖化液; 并使用糖化液体作为碳源培养微生物并生产生物乙醇。 Hydrodictyon家族藻类选自Hydrodictyon sp。，Pediastrum sp。和Sorastrum sp。 糖化使用水解催化剂，多糖分解酶或其混合物进行。 （附图标记）（AA）连续直接糖化/发酵过程; （BB）Hydrodictyon家庭藻类和藻类生物量; （CC，GG，NN）糖化酶; （DD）糖化; （EE）40-600℃，1天; （FF）藻类生物量; （HH，OO）乙醇生产菌株; （二）发酵; （JJ，QQ）30-40℃，3天; （KK，RR）生物乙醇; （LL）同时直接糖化/发酵过程; （MM）Hydrodictyon家庭藻类和藻类生物量（注射一次或两次或更多次）; （PP）糖化/发酵