公开(公告)号：KR1020150020453A

公开(公告)日：2015-02-26

申请号：KR1020130096907

申请日：2013-08-14

Applicant: 한국화학연구원

Inventor： 백진욱 ,  수밋챠우드리 ,  박노중 ,  라제스쿠마야다브

IPC: C12N9/02 ,  C12N9/96

Abstract: 본발명은광-바이오시스템을이용한산화환원효소보조인자의재생방법및 이를이용한효소반응으로케톤으로부터선택적으로키랄알코올을제조하는방법에관한것으로서, 본발명에따른그래핀계복합체를이용한산화환원효소보조인자의재생방법및 이를이용한키랄알코올화합물의제조방법은가시광선을흡수하는그래핀-BODIPY 광촉매, 산화환원매개체인로듐(Ⅲ) 복합체, 특정비극성유기용매, 최적화된바이오촉매인알코올디하이드로게나제및 이의보조인자를포함하는광-바이오시스템을사용함으로써, 산화환원효소의보조인자를 55%의높은효율로재생함과동시에, 이를이용한경제적이고환경친화적인효소작용을통하여케톤화합물로부터 95% 이상의높은광학순도를갖는키랄알코올화합물을제조할수 있으므로키랄알코올제조산업에유용하게사용될수 있다.

Abstract translation: 本发明涉及使用光生物系统的氧化还原酶辅因子的再生方法，以及使用该酶的酶反应的来自酮的手性醇的选择性制造方法。 使用石墨烯系复合体的氧化还原酶辅因子的再生方法以及使用本发明的手性醇化合物的制造方法使用光生物系统，其包含吸收可见光的石墨烯-BODIPY光催化剂，铑（III ）络合物，其是特殊的非极性有机溶剂，作为优化的生物催化剂的醇二氢基因酶及其辅助因子。 氧化还原酶辅因子可以通过使用光生物系统的经济和环境友好的酶反应，以55％的高效率和具有95％以上的光学纯度的酮化合物的手性醇化合物再生，从而具有 在手性酒精制造业中有用的应用。

公开(公告)号：KR1020130106242A

公开(公告)日：2013-09-27

申请号：KR1020120027998

申请日：2012-03-19

Applicant: 한국화학연구원

Inventor： 백진욱 ,  수밋챠우드리 ,  박노중 ,  라제스쿠마야다브 ,  김진흥

IPC: C07C31/00 ,  C07C31/34 ,  B01J21/18 ,  C07F15/00

Abstract: PURPOSE: A manufacturing method of a chiral alcohol compound is provided to regenerate the cofactor of an oxidoreductase with the high efficiency of 40% and to manufacture a chiral alcohol compound in a (R)-shape, thereby being with the optical activity of 80% from a ketone compound through an enzymatic reaction. CONSTITUTION: A manufacturing method of a chiral alcohol compound using a photo-biosystem comprises a step of a putting phosphorous-buffering solution, a cofactor of an oxidoreductase, an oxidation and reduction medium, a graphene-porphyrin optical catalyst, a non-polar organic solvent, an alcohol dehydrogenase, and a ketone derivative into a reactor and mixing the material while light is applied into the reactor under an inert gas atmosphere to conduct the reduction reaction of the ketone derivatives, thereby obtaining the chiral alcohol compound. The oxidation reduction medium is at least one selected from methyl viologen, a ruthenium (II) composite, and a rhodium (III) composite.

Abstract translation: 目的：提供手性醇化合物的制造方法，以高效率40％再生氧化还原酶的辅因子，并制备（R）形状的手性醇化合物，由此光学活性为80％ 从酮化合物通过酶反应。 构成：使用光生物系统的手性醇化合物的制造方法包括放入磷缓冲溶液，氧化还原酶的辅因子，氧化还原介质，石墨烯 - 卟啉光学催化剂，非极性有机物 溶剂，醇脱氢酶和酮衍生物混合到反应器中，并在惰性气体气氛下将光混合到反应器中进行混合，进行酮衍生物的还原反应，得到手性醇化合物。 氧化还原培养基是选自甲基紫精，钌（II）复合物和铑（III）复合物中的至少一种。

公开(公告)号：KR1020130102422A

公开(公告)日：2013-09-17

申请号：KR1020120023645

申请日：2012-03-07

Applicant: 한국화학연구원

Inventor： 백진욱 ,  오규환 ,  박노중 ,  라제스쿠마야다브 ,  김진흥

IPC: C01B31/02 ,  C01G55/00 ,  B01J19/18

CPC classification number: C01B32/182 ,  B01J19/18 ,  B01J21/18 ,  C01G55/00

Abstract: PURPOSE: A novel graphene photocatalyst absorbing visible light, a regeneration method of oxidoreductase cofactor using the same, and a manufacturing method of formic acid from carbon dioxide with an enzyme reaction using the same are provided to be economic and environment-friendly, to enable the mass production and automation, and to be able to be usefully used in a novel manufacture industry in which artificial photosynthesis which selectively manufactures various compounds by fixing carbon dioxide which is global warming gas. CONSTITUTION: A novel graphene composite is represented by Chemical formula 1. The novel graphene composite is used as a photocatalyst absorbing visible light. A regeneration method of oxidoreductase cofactor comprises a step of inputting a phosphate buffer solution; oxidative oxidoreductase cofactor methyl viologen; at least one oxidation-reduction medium selected from the group consisting of a ruthenium (II) composite and a rhodium (III) composite; and the graphene photocatalyst absorbing visible light represented by Chemical formula 1 in a reactor, and stirring while applying light to produce a reductive oxidoreductase cofactor.

Abstract translation: 目的：吸收可见光的新型石墨烯光催化剂，使用其的氧化还原酶辅因子的再生方法和使用其的酶反应的来自二氧化碳的甲酸的制备方法是经济和环境友好的，以使 大规模生产和自动化，并且能够有效地用于通过固定作为全球变暖气体的二氧化碳选择性地制造各种化合物的人造光合作用的新型制造业。 构成：新型石墨烯复合材料由化学式1表示。新型石墨烯复合材料用作吸收可见光的光催化剂。 氧化还原酶辅因子的再生方法包括输入磷酸盐缓冲溶液的步骤; 氧化还原酶辅助因子甲基紫精; 至少一种选自钌（II）复合物和铑（III））复合材料的氧化还原介质; 以及在反应器中吸收由化学式1表示的可见光的石墨烯光催化剂，并在施加光时进行搅拌以产生还原性氧化还原酶辅因子。

公开(公告)号：KR1020120026656A

公开(公告)日：2012-03-20

申请号：KR1020100088662

申请日：2010-09-10

Applicant: 한국화학연구원

Inventor： 백진욱 ,  오규환 ,  박노중 ,  황동원 ,  라제스쿠마야다브 ,  김진흥

IPC: C07D487/22 ,  C07D251/54 ,  C07D403/14 ,  B01J31/02

CPC classification number: C07D487/22 ,  B01J31/22 ,  C01B32/182 ,  C07D251/54 ,  C07D403/14

Abstract: PURPOSE: A novel visible light absorption grapheme-porphyrin photocatalyst and a method for regenerating oxidoreductase cofactors are provided to save energy. CONSTITUTION: A novel graphene-porphyrin complex is denoted by chemical formula 1. A visible light absorptioin grapheme-porphyrin photocatalyst contains the novel grapheme-porphyrin complex as an active ingredient. A method for regenerating oxidorductase cofactors comprises: a step of mixing phosphate buffer, oxidative oxidoreductase cofactor, methyl viologen, luthenium(II) complex or rhodium(III) complex and the photocatalyst in a reactor; and a step of applying light and stirring. The cofactor is NAD+, NADP+, FAD+ or FMN+.

Abstract translation: 目的：提供一种新颖的可见光吸收型字形卟啉光催化剂和一种再生氧化还原酶辅因子的方法，以节约能源。 构成：新型石墨烯 - 卟啉复合物由化学式1表示。可见光吸收光谱por卟啉卟啉光催化剂含有新颖的grap卟啉复合物作为活性成分。 用于再生氧化酶辅因子的方法包括：在反应器中混合磷酸盐缓冲液，氧化还原酶辅因子，甲基紫精，钌（II）络合物或铑（III）络合物和光催化剂的步骤; 以及应用光和搅拌的步骤。 辅因子是NAD +，NADP +，FAD +或FMN +。