公开(公告)号：KR1020120097859A

公开(公告)日：2012-09-05

申请号：KR1020110017356

申请日：2011-02-25

Applicant: 한국화학연구원

Inventor： 윤석주 ,  황승용 ,  오정화 ,  박한진 ,  김승준 ,  오문주

IPC: C12Q1/68 ,  C12N15/12 ,  C40B40/08

CPC classification number: C12Q1/6888 ,  C12Q1/6837 ,  C12Q2600/142 ,  C12Q2600/158 ,  C12Q2600/178

Abstract: PURPOSE: A microarray chip for detecting reproductive toxicant induced by endocrine disruptor and a kit for detecting reproductive toxicant are provided to identify reproductive toxicity mechanism and to study ecotoxicogenomics. CONSTITUTION: A microarray chip for detecting reproductive toxicant induced by endocrine disruptor contains a biomarker. The biomarker is an oligonucleotide containing 15-50 nucleic acids or complementary strand molecule thereof. A method for detecting the biomarker to identify reproductive toxicity induced by endocrine disruptor comprises: a step of synthesizing cDNA from RNA samples isolated from regenerative cells of an experimental group(male individual which is suspicious for endocrine disruptor exposure) and a control group; a step of labeling the cDNA with different fluorescent substances; a step of hybridizing the cDNA with the DNA microarray chip; a step of analyzing the DNA microarray chip; and a step of comparing gene expression level of the DNA microarray chip between the experimental group and control group. [Reference numerals] (AA) Reproduction; (BB) Cell proliferation; (CC) Signal transduction; (DD) Cell adhesion; (EE) Cell cycle; (FF) Immune response; (GG) Inflammatory response; (HH) Cell apoptosis; (II) Transport; (JJ) Lipid metabolism; (KK) Protein synthesis; (LL) Gene transcription; (MM) Homeostasis; (NN) Cell growth; (OO) Cell differentiation

Abstract translation: 目的：提供用于检测内分泌干扰物引起的生殖毒素的微阵列芯片和用于检测生殖毒物的试剂盒，以鉴定生殖毒性机制和研究生态毒性基因组学。 构成：用于检测内分泌干扰物引起的生殖毒物的微阵列芯片含有生物标志物。 生物标志物是含有15-50个核酸或其互补链分子的寡核苷酸。 用于检测生物标志物以鉴定由内分泌干扰物引起的生殖毒性的方法包括：从实验组（对于内分泌干扰物暴露的可疑的雄性个体）的再生细胞分离的RNA样品和对照组合成cDNA的步骤; 用不同荧光物质标记cDNA的步骤; 将cDNA与DNA微阵列芯片杂交的步骤; 分析DNA微阵列芯片的步骤; 并比较实验组与对照组之间的DNA芯片芯片的基因表达水平。 （附图标记）（AA）繁殖; （BB）细胞增殖; （CC）信号转导; （DD）细胞粘附; （EE）细胞周期; （FF）免疫反应; （GG）炎症反应; （HH）细胞凋亡; （二）运输; （JJ）脂质代谢; （KK）蛋白质合成; （LL）基因转录; （MM）稳态; （NN）细胞生长; （OO）细胞分化

公开(公告)号：KR1020110069448A

公开(公告)日：2011-06-23

申请号：KR1020090126188

申请日：2009-12-17

Applicant: 한국화학연구원

Inventor： 윤석주 ,  황승용 ,  김승준 ,  오문주 ,  오정화 ,  박한진

IPC: C12Q1/68 ,  C12N15/12 ,  C12N5/076

CPC classification number: C12Q1/6837 ,  C12N5/061 ,  C12N15/11 ,  C12Q1/6827

Abstract: PURPOSE: A method for detecting bisphenol A using reproductive system chromosome aberration is provided to determine bisphenol A presence in a test sample. CONSTITUTION: A method for detecting bisphenol A(BPA) of genetic toxic level in a test sample comprises: a step of isolating genomic DNA from reproductive cells of a male experimental animal except for human body; a step of measuring copy number from the genomic DNA; and a step of confirming copy number variation(CNV) in the genome. The reproductive cell is derived from testis or epididymis. The animal is rat, mouse, rabbit, guinea pig, hamster, dog, or cat. In case of a rat or mouse, the CNV exists in Chr2:77739470-77848433, Chr5:128329169-128329869, Chr7:7312289-10272836, Chr9:101951419-101951819, Chr10:72178606-72179006, Chr12:17803411-25366481, or Chr14:4551029-10397399. A gene relating to pheromone reaction is Vomeronasal 1 receptor, J2, E11, L1, G2, G3, G4, G5, G6, G7, G11, or K1.

Abstract translation: 目的：提供使用生殖系统染色体畸变检测双酚A的方法，以确定测试样品中双酚A的存在。 构成：测试样品中检测双酚A（BPA）的遗传毒性水平的方法包括：从除人体外的雄性实验动物的生殖细胞中分离基因组DNA的步骤; 从基因组DNA测量拷贝数的步骤; 以及确认基因组中拷贝数变异（CNV）的步骤。 生殖细胞来源于睾丸或附睾。 动物是大鼠，小鼠，兔，豚鼠，仓鼠，狗或猫。 在大鼠或小鼠的情况下，CNV存在于Chr2：77739470-77848433，Chr5：128329169-128329869，Chr7：7312289-10272836，Chr9：101951419-101951819，Chr10：72178606-72179006，Chr12：17803411-25366481或Chr14 ：4551029-10397399。 与信息素反应相关的基因是犁鼻1受体，J2，E11，L1，G2，G3，G4，G5，G6，G7，G11或K1。