SELECTIVE THIN-FILM CULTURE DEVICE FOR ENUMERATING MICROORGANISMS

    公开(公告)号:US20210040434A1

    公开(公告)日:2021-02-11

    申请号:US16977244

    申请日:2019-05-02

    Abstract: A selective microbial detection device and methods of use are provided. The device includes a water-proof pouch that includes a first wall portion, a second wall portion, and a porous membrane filter disposed in the pouch between the first and second wall portions. The filter membrane divides the pouch into first and second compartments. The microbial detection device also includes an effective amount of a dry nutrient disposed in the first compartment, which contains sodium lauryl sulfate in an amount of 1.75 milligrams or greater per twelve square inches of the first wall portion. A dry, cold water-soluble gelling agent is adhered to the pouch in the first compartment and an absorbent pad is disposed in the second compartment. A sealable sample port provides access to deposit a liquid into the first compartment.

    Method of enumerating coliform colonies

    公开(公告)号:US10662460B2

    公开(公告)日:2020-05-26

    申请号:US15781547

    申请日:2016-12-06

    Inventor: Patrick A. Mach

    Abstract: A method of enumerating gas-producing coliform bacteria present in a cultured dairy product is provided. The method can comprise forming a mixture that comprises a predefined quantity of the dairy product and a predefined volume of a diluent; inoculating a thin film culture device with the mixture to form a hydrogel comprising a nutrient medium for growing coliform bacteria, an effective concentration of lactose, and an effective concentration of MOPS; incubating the inoculated thin film culture device under conditions that facilitate growth of a coliform bacterium; and identifying and counting a gas-producing colony present in the inoculated culture device.

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