公开(公告)号：EP0974060A1

公开(公告)日：2000-01-26

申请号：EP98910389.0

申请日：1998-03-13

Applicant: Abbott Laboratories

Inventor： LEE, Evelyn, Mok ,  WESTERBERG, David, A. ,  YAO, Haiou, H. ,  ADAMCZYK, Janina ,  CHRISTENSEN, Melissa, A.

IPC: G01N33/72 ,  G01N33/543

CPC classification number: G01N33/723 ,  Y10S435/96 ,  Y10S436/805 ,  Y10S436/824

Abstract: A glycated hemoglobin assay utilizes a simple procedure for the determination of DCCT Standardized %GHb in whole blood samples. First, a lysed whole blood sample is incubated with a solid phase that is coupled with boronic acid or similar boronate compound through covalent linkage chemistries known in the art. Next, a labeled antibody to human hemoglobin is added and the resulting signal is directly proportional to the %GHb in the sample. The advantages of measuring %GHb using a single determination include high precision and, since the assay is easily automatable, high throughput. With automation, this assay can also be consolidated with other testing on one analyzer. The method according to the various embodiments of the invention thus eliminates the need for two measurements: one for GHb and another for total hemoglobin (THb).

公开(公告)号：EP0586605B1

公开(公告)日：1998-08-05

申请号：EP92914861.7

申请日：1992-04-10

Applicant: ABBOTT LABORATORIES

Inventor： ADAMCZYK, Janina ,  BERRY, Daniel, S. ,  FICO, Rosario ,  JOU, Yi-Her ,  STROUPE, Stephen, D.

IPC: C12Q1/00 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/536 ,  G01N33/537 ,  G01N33/538 ,  G01N33/541 ,  G01N33/543 ,  G01N33/544 ,  G01N33/546 ,  G01N33/532 ,  G01N33/545

CPC classification number: G01N33/538

Abstract: This invention presents novel assay methods employing a capture reagent, involving a first binding member typically conjugated to a polymeric anion substance, and a solid phase material containing a reaction site comprising a polymeric cation substance having a nitrogen content of at least about two percent. In one embodiment a test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of a polycationic substance, having a nitrogen content of at least about two percent (excluding the counter ions), to prepare a suitably charged solid phase provides a solid phase that can be subjected to a greater extent of manipulation, such as multiple washes, without losing the capability to attract and retain the capture reagent.

公开(公告)号：EP0641388A1

公开(公告)日：1995-03-08

申请号：EP92912697.0

申请日：1992-04-10

Applicant: ABBOTT LABORATORIES

Inventor： ADAMCZYK, Janina ,  BERRY, Daniel, S. ,  JOU, Yi-Her ,  STROUPE, Stephen, Denham

IPC: G01N33

CPC classification number: G01N33/538 ,  G01N33/54353 ,  G01N33/548

Abstract: The present invention includes novel assays employing a capture reagent, involving a first binding member conjugated to a polymeric anion such as carboxymethylamylose, and a solid phase material containing a reaction site comprising a polymeric cation substance. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of carboxymethylamylose to prepare a suitably charged capture reagent provides a superior capture reagent that is capable of binding and retaining the analyte on the solid phase even in the presence of polyanionic non-specific binding blockers.