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    METHOD FOR PRODUCING A MARKER-FREE MUTATED TARGET ORGANISM AND PLASMID VECTORS SUITABLE FOR THE SAME
    14.
    发明申请
    METHOD FOR PRODUCING A MARKER-FREE MUTATED TARGET ORGANISM AND PLASMID VECTORS SUITABLE FOR THE SAME 审中-公开
    用于生产无标记的突变靶有机体合适的质粒

    公开(公告)号：WO03014362A3

    公开(公告)日：2003-05-01

    申请号：PCT/EP0208231

    申请日：2002-07-24

    Applicant: BASF AG ,  POMPEJUS MARKUS ,  KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  LIEBL WOLFGANG

    Inventor： POMPEJUS MARKUS ,  KLOPPROGGE CORINNA ,  ZELDER OSKAR ,  LIEBL WOLFGANG

    IPC: C12N15/09 ,  C12N1/21 ,  C12N15/54 ,  C12N15/77

    CPC classification number: C12N15/77

    Abstract: The invention relates to a plasmid vector which does not replicate in a target organism and contains the following constituents: a) an origin of replication for a host organism which is not identical to the target organism, b) at least one genetic marker, c) optionally a section of a sequence which enables the transfer of DNA by means of conjugation (mob sequence), d) a section of a sequence which is homologous to sequences of the target organism and enables homologous recombination in the target organism, e) a gene for a galactokinase under the control of a promoter.

    Abstract translation: 本发明涉及不在目标生物体中复制，包括以下组分的质粒载体：对于宿主生物体，其是不相同的目标生物体，b）至少一个遗传标记，c）任选的序列部分中的）复制起点（复制起点） 该DNA的通过接合转移允许（MOB序列），D）的序列部分，其是同源的靶生物的序列，并允许靶生物体的同源重组，e）在一个启动子的控制下的半乳糖激酶的基因。

    USE OF DIMETHYL DISULFIDE FOR METHIONINE PRODUCTION IN MICROORGANISMS
    16.
    发明申请
    USE OF DIMETHYL DISULFIDE FOR METHIONINE PRODUCTION IN MICROORGANISMS 审中-公开
    二甲基磺脲在微生物中的生产中的应用

    公开(公告)号：WO2007011939A3

    公开(公告)日：2007-07-05

    申请号：PCT/US2006027855

    申请日：2006-07-18

    Applicant: BASF AG ,  ZELDER OSKAR ,  HAEFNER STEFAN ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  YOCUM R ROGERS ,  WILLIAMS MARK K

    Inventor： ZELDER OSKAR ,  HAEFNER STEFAN ,  HEROLD ANDREA ,  KLOPPROGGE CORINNA ,  SCHRODER HARTWIG ,  YOCUM R ROGERS ,  WILLIAMS MARK K

    IPC: C12P13/12

    CPC classification number: C12P13/12

    Abstract: The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a ?metF organism or a ?metE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/Met? and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A ?metF ?metB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

    Abstract translation: 本发明的特征在于用于生产甲硫氨酸的改进方法和生物体。 本发明证明，metF生物体或αmetE AmetH生物体，例如谷氨酸棒状杆菌或大肠杆菌的突变体可以使用甲基封端的硫化物源，例如二甲基二硫（DMDS）作为硫的来源 和甲基，绕过MetH / Met？ 和MetF活性以及减少硫酸盐的需要，用于合成甲硫氨酸。 该专利中还描述了涉及MetY（也称为MetZ）的数据，其作为将甲基封端的硫化物源（例如DMDS）并入甲硫氨酸中的酶。 谷氨酸棒状芽孢杆菌的ΔFIFFmetB菌株可以使用甲基封端的硫化物源，例如DMDS，作为硫化物和甲基两者的来源。 此外，通过加入甲基封端的硫化物源（例如DMDS），改善过度产生O-乙酰高丝氨酸的工程化原养殖生物体的甲硫氨酸生产。

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