公开(公告)号：CA2564661A1

公开(公告)日：2005-12-01

申请号：CA2564661

申请日：2005-05-11

Applicant: BASF AG

Inventor： HOLLMANN FRANK ,  SCHMID ANDREAS ,  HAUER BERNHARD ,  HABICHER TILO ,  HOFSTETTER KARIN

IPC: C12P1/00 ,  C12N9/02 ,  C12P7/00

Abstract: Process for an enzymatic oxygenation catalyzed by a FAD-dependant monooxygenase and direct electrochemical regeneration of the FAD-dependant monooxygenase.

公开(公告)号：AU782371B2

公开(公告)日：2005-07-21

申请号：AU1388001

申请日：2000-10-26

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  WITHOLT BERNARD ,  HAUER BERNHARD ,  BUHLER BRUNO

IPC: C12N15/09 ,  C12N1/21 ,  C12P7/24 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38

Abstract: Production of aromatic aldehyde or carboxylic acid (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate (II). Production of aromatic aldehyde or carboxylic acid of formula (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate of formula (II). [Image] Ar : monocyclic aromatic ring, optionally with one or more substituents; R 1>formyl or carboxy; n : 0-15; R 2>vinyl or (CH 2) m-R 3>, also where R 1>= carboxy, R 2>may be (CH 2) n-CHO; m : n+1; and R 3>hydrogen or hydroxy. Independent claims are also included for the following: (a) recombinant microorganism harboring an expression vector that contains, under control of the alk-regulatory system of Pseudomonas oleovoransGpol, the XMO-encoding genes xylM and xylA, or the AMO-encoding genes alkB, alkG and alkT; and (b) expression construct containing the genes specified in (a), operably linked to the alk-regulatory system.

公开(公告)号：EE200200646A

公开(公告)日：2004-06-15

申请号：EEP200200646

申请日：2001-05-16

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  HOLLMANN FRANK ,  HAUER BERNHARD ,  ZELINSKI THOMAS

IPC: C12M1/40 ,  C12P1/00 ,  C12P7/22 ,  C25B3/25 ,  C12M1/00 ,  C25B3/04 ,  C25B9/00

Abstract: A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD + formed by reductive cleavage of oxygen is electrochemically reduced, is described.

公开(公告)号：ZA200210128B

公开(公告)日：2004-03-10

申请号：ZA200210128

申请日：2002-12-13

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  HOLLMANN FRANK ,  SCHMID ANDREAS ,  STECKHAN EBERHARD ,  ZELINSKI THOMAS

IPC: C12P1/00 ,  C12P7/22 ,  C25B3/25 ,  C12M1/40 ,  C12P ,  C25B ,  C12M

Abstract: A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD + formed by reductive cleavage of oxygen is electrochemically reduced, is described.

公开(公告)号：NO20025503A

公开(公告)日：2002-11-15

申请号：NO20025503

申请日：2002-11-15

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  HOLLMANN FRANK ,  HAUER BERNHARD ,  ZELINSKI THOMAS ,  STECKHAN EBERHARD

IPC: C12M1/40 ,  C12P1/00 ,  C12P7/22 ,  C12M1/00 ,  C25B3/04 ,  C25B9/00

CPC classification number: C12P1/00

公开(公告)号：CA2442561A1

公开(公告)日：2002-10-17

申请号：CA2442561

申请日：2002-04-05

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  ZELINSKI THOMAS ,  SCHMID ANDREAS ,  WITHOLT BERNHARD ,  BUHLER BRUNO

IPC: C12N15/09 ,  C12P7/22 ,  C12P7/24 ,  C12P7/40 ,  C12R1/40

Abstract: The invention relates to a method for the biocatalytic oxidation of aromatic compounds, using recombinant micro-organisms expressing xylene monooxygenase , in a two-phase reaction medium.

公开(公告)号：AU1388001A

公开(公告)日：2001-05-08

申请号：AU1388001

申请日：2000-10-26

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  WITHOLT BERNARD ,  HAUER BERNHARD ,  BUHLER BRUNO

IPC: C12N15/09 ,  C12N1/21 ,  C12P7/24 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38

Abstract: Production of aromatic aldehyde or carboxylic acid (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate (II). Production of aromatic aldehyde or carboxylic acid of formula (I) by culturing a microorganism that expresses xylene mono-oxygenase (XMO) or alkane mono-oxygenase (AMO) in a medium containing an aromatic substrate of formula (II). [Image] Ar : monocyclic aromatic ring, optionally with one or more substituents; R 1>formyl or carboxy; n : 0-15; R 2>vinyl or (CH 2) m-R 3>, also where R 1>= carboxy, R 2>may be (CH 2) n-CHO; m : n+1; and R 3>hydrogen or hydroxy. Independent claims are also included for the following: (a) recombinant microorganism harboring an expression vector that contains, under control of the alk-regulatory system of Pseudomonas oleovoransGpol, the XMO-encoding genes xylM and xylA, or the AMO-encoding genes alkB, alkG and alkT; and (b) expression construct containing the genes specified in (a), operably linked to the alk-regulatory system.

公开(公告)号：ES2258982T3

公开(公告)日：2006-09-16

申请号：ES00975925

申请日：2000-10-26

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  WITHOLT BERNARD ,  HAUER BERNHARD ,  BUHLER BRUNO

IPC: C12N15/09 ,  C12P7/24 ,  C12N1/21 ,  C12P7/40 ,  C12R1/19 ,  C12R1/38

Abstract: Un proceso para la preparación de aldehídos aromáticos y/o de ácidos carboxílicos de la fórmula I Ar-(CH2)n-R1 (I) donde Ar es un anillo aromático substituido o mono- o polisubstituido mononuclear, R1 es un grupo -CHO o ¿COOH que contiene oxígeno, y n es un número entero de 0 a 15, caracterizado porque comprende a) un medio de cultivo que abarca un substrato aromático de la fórmula II Ar-R2 (II) donde Ar tiene el significado definido anteriormente y R2 es -CH=CH2 o -(CH2)n+1R3, donde n tiene el significado definido anteriormente y R3 es H u OH; o, si R1 es -COOH, R2 también puede ser - (CH2)nR4, donde n tiene el significado definido anteriormente y R4 es ¿CHO; un microorganismo que expresa el xileno monooxigenasa (XMO), donde el XMO expresado cataliza la oxidación del substrato de la fórmula II a un compuesto de la fórmula I, y b) que aísla el compuesto(s) de la fórmula I del medio de cultivo, donde el microorganismo que expresa XMO no presenta esencialmente ninguna actividad de betaínaaldehído deshidrogenasa (BADH) y, opcionalmente, de deshidrogenasa del benzaldehído (BZDH).

公开(公告)号：NO20025503D0

公开(公告)日：2002-11-15

申请号：NO20025503

申请日：2002-11-15

Applicant: BASF AG

Inventor： SCHMID ANDREAS ,  HOLLMANN FRANK ,  HAUER BERNHARD ,  ZELINSKI THOMAS ,  STECKHAN EBERHARD

IPC: C12P1/00 ,  C12M1/40 ,  C12P7/22 ,  C25B3/25 ,  C12P

Abstract: A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NAD + formed by reductive cleavage of oxygen is electrochemically reduced, is described.

公开(公告)号：DE10117359A1

公开(公告)日：2002-10-10

申请号：DE10117359

申请日：2001-04-06

Applicant: BASF AG

Inventor： HAUER BERNHARD ,  ZELINSKI THOMAS ,  WITHOLT BERNHARD ,  SCHMID ANDREAS ,  BUEHLER BRUNO

IPC: C12N15/09 ,  C12P7/22 ,  C12P7/24 ,  C12P7/40 ,  C12R1/40 ,  C07C47/542 ,  C07C47/228 ,  C07C33/20 ,  C07C39/02 ,  C07C63/06 ,  C07C57/30

Abstract: The invention relates to a method for the biocatalytic oxidation of aromatic compounds, using recombinant micro-organisms expressing xylene monooxygenase, in a two-phase reaction medium.