公开(公告)号：US20240367141A1

公开(公告)日：2024-11-07

申请号：US18652860

申请日：2024-05-02

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： BRIAN PANGANIBAN ,  SHAWN DARNALL ,  DANNI WANG ,  CHRISTOPHER BELISLE

IPC: B01J20/04 ,  B01D15/20 ,  B01D15/42 ,  B01J20/282 ,  B01J20/30 ,  B01J20/32

Abstract: This disclosure relates to particulate hydroxyapatite (HA)-coated substrates, methods of making particulate HA-coated substrates, and methods of using particulate HA-coated substrates. Thus, the disclosure provides chromatography media composed of a particulate substrate (such as polymer beads or other particulate substrates) that is coated with HA. Also provided is a chromatography media (optionally contained within a column) comprising a HA-coated substrate (e.g., a plurality of beads or another particulate substrate that is coated with HA). The beads or particulate substrate can be a porous or non-porous substrate.

公开(公告)号：US20240001338A1

公开(公告)日：2024-01-04

申请号：US18360870

申请日：2023-07-28

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： CHRISTOPHER BELISLE ,  JIALI LIAO ,  EDWIN LOZANO

IPC: B01J20/285 ,  B01J20/289 ,  B01J20/30 ,  C07K1/16 ,  B01D15/38 ,  B01J20/28

CPC classification number: B01J20/285 ,  B01J20/289 ,  B01J20/3042 ,  C07K1/165 ,  B01D15/3847 ,  B01D15/3809 ,  B01J20/28085 ,  B01J2220/52

Abstract: The subject invention pertains to proteins are purified by a mixed-mode chromatography system formed by attaching a ligand with cation exchange and hydrophobic 1,3-dioxoisoindolin-2-yl group functionalities to a large-pore support matrix, the only linkage between the ligand and the support matrix being a chain having a backbone of one, two, three, four, or five atoms between the hydrophobic group and the support matrix.