公开(公告)号：CA2786916A1

公开(公告)日：2011-07-21

申请号：CA2786916

申请日：2011-01-14

Applicant: UNIV BRITISH COLUMBIA ,  FLUIDIGM CORP

Inventor： LIVAK KENNETH J ,  HEYRIES KEVIN ,  HANSEN CARL L G ,  PETRIV OLEH

IPC: C12Q1/68 ,  C12P19/34 ,  C40B30/04 ,  G01N33/50

Abstract: Kits, primers, and methods are provided herein for detecting relative target source to reference source ratios in a biological sample, by distributing the biological sample into discrete subsamples, wherein the biological sample includes, a plurality of target molecules on a target source; and a plurality of reference molecules on a reference source; providing target primers directed to one or more of the plurality of target molecules and reference primers directed to one or more of the plurality of reference molecules; performing digital amplification with the target primers and the reference primers; and detecting the presence or absence of amplified target products with target probes and detecting the presence or absence of amplified reference products with reference probes, wherein the ratio of amplified target products to amplified reference products is indicative of a relative amount of target source to reference source in a biological sample.