公开(公告)号：EP3390658A1

公开(公告)日：2018-10-24

申请号：EP16820542.5

申请日：2016-12-16

Applicant: Fluidigm Corporation

Inventor： CHEN, Peilin

IPC: C12Q1/68

CPC classification number: B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00722 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2525/161 ,  C12Q2525/301

Abstract: The present disclosure provides a “looping amplification” method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.

公开(公告)号：EP2852682B1

公开(公告)日：2017-10-04

申请号：EP13726975.9

申请日：2013-05-21

Applicant: Fluidigm Corporation

Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

IPC: C12Q1/68

CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.

Abstract translation: 在某些实施方案中，本发明提供了用于测定颗粒群中的单个颗粒的方法和装置，其中对于每个颗粒测量至少两个参数。 当粒子处于单独的反应体积中时，可以测量一个或多个参数。 可替代地或另外地，可以在稍后的分析步骤中测量一个或多个参数，例如，在单独的反应体积中进行反应并且回收和分析反应产物。 在特定的实施方案中，“并行”进行一个或多个参数测量，即基本上同时在单独的反应体积中进行。

公开(公告)号：EP2710172B1

公开(公告)日：2017-03-29

申请号：EP12789957.3

申请日：2012-05-21

Applicant: Fluidigm Corporation

Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  JONES, Robert C. ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

公开(公告)号：EP2414547A1

公开(公告)日：2012-02-08

申请号：EP10759511.8

申请日：2010-04-02

Applicant: Fluidigm Corporation

Inventor： MAY, Andrew ,  CHEN, Peilin ,  WANG, Jun ,  KAPER, Fiona ,  ANDERSON, Megan

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.