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    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    11.
    发明公开
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 审中-公开

    公开(公告)号：EP3627140A1

    公开(公告)日：2020-03-25

    申请号：EP19189921.0

    申请日：2013-02-28

    Applicant: Fluidigm Corporation

    Inventor： FOWLER, Brian ,  KIMBALL, Jake ,  MAUNG, Myo Thu ,  MAY, Andrew ,  NORRIS, Michael C. ,  TOPPANI, Dominique G. ,  UNGER, Marc A. ,  WANG, Jing ,  WEST, Jason A. A.

    IPC: G01N15/10 ,  C12Q1/6806

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    12.
    发明公开
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 有权
    EINZELPARTIKELANALYSE VON TEILCHENPOPULATIONEN

    公开(公告)号：EP2852682A2

    公开(公告)日：2015-04-01

    申请号：EP13726975.9

    申请日：2013-05-21

    Applicant: Fluidigm Corporation

    Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    IPC: C12Q1/68

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供了用于测定颗粒群体中的单个颗粒的方法和装置，其中针对每个颗粒测量至少两个参数。 当颗粒处于分开的反应体积中时，可以测量一个或多个参数。 或者或另外，可以在稍后的分析步骤中测量一个或多个参数，例如其中在分开的反应体积中进行反应并回收和分析反应产物。 在具体实施方案中，一个或多个参数测量“并行地进行”，即基本上同时在单独的反应体积中进行。

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    15.
    发明公开
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 审中-公开
    使用微流控芯片进行多个单细胞捕获和处理的方法，系统和设备

    公开(公告)号：EP3285062A1

    公开(公告)日：2018-02-21

    申请号：EP17180284.6

    申请日：2013-02-28

    Applicant: Fluidigm Corporation

    Inventor： FOWLER, Brian ,  KIMBALL, Jake ,  MAUNG, Myo Thu ,  MAY, Andrew ,  NORRIS, Michael C. ,  TOPPANI, Dominique G. ,  UNGER, Marc A. ,  WANG, Jing ,  WEST, Jason A. A.

    IPC: G01N15/10

    CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    Abstract translation: 描述了使用微流体的多个单细胞捕获和处理的方法，系统和设备。 提供了用于捕获，分配和/或操纵来自更大细胞群体的个体细胞以及产生与每个个体细胞相关的遗传信息和/或反应的工具和技术。 可以使用不同的捕获配置来捕获单个细胞，然后在多腔室反应配置中处理每个单独的细胞。 一些实施例可以提供特定目标扩增，全基因组扩增，全转录扩增，实时PCR制备，拷贝数变异，预扩增的mRNA的测序，和/或单倍型分析已经从较大人口划分的多个单个细胞的 细胞。 一些实施例可以提供其他应用。 一些实施例可以被配置用于将单独的单元或相关联的反应产物成像为处理的一部分。 反应产物可能在一些情况下收获和/或进一步分析。

    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS
    16.
    发明授权
    SINGLE-PARTICLE ANALYSIS OF PARTICLE POPULATIONS 有权
    粒子群的单粒子分析

    公开(公告)号：EP2852682B1

    公开(公告)日：2017-10-04

    申请号：EP13726975.9

    申请日：2013-05-21

    Applicant: Fluidigm Corporation

    Inventor： ANDERSON, Megan ,  CHEN, Peilin ,  FOWLER, Brian ,  KAPER, Fiona ,  LEBOFSKY, Ronald ,  MAY, Andrew

    IPC: C12Q1/68

    CPC classification number: C12Q1/6813 ,  B01D15/08 ,  C12Q1/6806 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the invention provides methods and devices for assaying single particles in a population of particles, wherein at least two parameters are measured for each particle. One or more parameters can be measured while the particles are in the separate reaction volumes. Alternatively or in addition, one or more parameters can be measured in a later analytic step, e.g., where reactions are carried out in the separate reaction volumes and the reaction products are recovered and analyzed. In particular embodiments, one or more parameter measurements are carried out “in parallel,” i.e., essentially simultaneously in the separate reaction volumes.

    Abstract translation: 在某些实施方案中，本发明提供了用于测定颗粒群中的单个颗粒的方法和装置，其中对于每个颗粒测量至少两个参数。 当粒子处于单独的反应体积中时，可以测量一个或多个参数。 可替代地或另外地，可以在稍后的分析步骤中测量一个或多个参数，例如，在单独的反应体积中进行反应并且回收和分析反应产物。 在特定的实施方案中，“并行”进行一个或多个参数测量，即基本上同时在单独的反应体积中进行。

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