公开(公告)号：EP3627140A1

公开(公告)日：2020-03-25

申请号：EP19189921.0

申请日：2013-02-28

Applicant: Fluidigm Corporation

Inventor： FOWLER, Brian ,  KIMBALL, Jake ,  MAUNG, Myo Thu ,  MAY, Andrew ,  NORRIS, Michael C. ,  TOPPANI, Dominique G. ,  UNGER, Marc A. ,  WANG, Jing ,  WEST, Jason A. A.

IPC: G01N15/10 ,  C12Q1/6806

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：EP2074341A2

公开(公告)日：2009-07-01

申请号：EP07843872.8

申请日：2007-10-04

Applicant: Fluidigm Corporation

Inventor： WANG, Jing ,  NASSEF, Hany, R.

IPC: F16K1/00 ,  F16K15/00

CPC classification number: F16K99/0026 ,  B01L3/502738 ,  B01L2200/12 ,  B01L2300/0887 ,  B01L2400/0605 ,  B01L2400/0638 ,  F16K99/0001 ,  F16K99/0057 ,  F16K2099/0078 ,  F16K2099/008

Abstract: A microfluidic check valve and a method of using the check valve In microfluidic devices includes a check valve comprising two stacked chambers that are separated by a pore-containing membrane. The membrane is composed of an elastomeric material and can be configured in normally open or normally closed state. The normally open check valve can be implemented so that the degree of back pressure necessary to close the valve can be set.

公开(公告)号：EP2125219A2

公开(公告)日：2009-12-02

申请号：EP08728100.2

申请日：2008-01-22

Applicant: Fluidigm Corporation

Inventor： COHEN, David, S. ,  WANG, Jing ,  MAY, Andrew, P. ,  JONES, Robert, C. ,  NASSEF, Hany

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/5025 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0638 ,  B01L2400/0655 ,  B33Y80/00 ,  F16K99/0001 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T137/0318 ,  Y10T137/85938

Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.

Abstract translation: 新的高密度微流体装置和方法提供流体体积的精确计量和计量体积的有效混合。 将第一溶液引入到与反应室流体连通的流动通道的段中。 第二溶液流过该段，使得第一溶液移入反应室，并且第二溶液的体积进入室。 然后可以分离室，并且可以启动和/或检测室内的反应。 遗传分析的高通量方法可以以比以前更高的精度进行。

公开(公告)号：EP2820394B1

公开(公告)日：2017-07-12

申请号：EP13754093.6

申请日：2013-02-28

Applicant: Fluidigm Corporation

Inventor： FOWLER, Brian ,  KIMBALL, Jake ,  MAUNG, Myo Thu ,  MAY, Andrew ,  NORRIS, Michael C. ,  TOPPANI, Dominique G. ,  UNGER, Marc A. ,  WANG, Jing ,  WEST, Jason A. A.

IPC: G01N15/10

CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

公开(公告)号：EP2506882A1

公开(公告)日：2012-10-10

申请号：EP10834067.0

申请日：2010-11-30

Applicant: Fluidigm Corporation

Inventor： WANG, Jing ,  WOUDENBERG, Timothy M.

IPC: A61L2/00

CPC classification number: B08B9/027 ,  B01L3/5027 ,  B01L99/00 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0655 ,  F16K99/0001 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084

Abstract: A method for rendering a microfluidic device suitable for reuse for nucleic acid analysis is provided. The method may include flowing a nucleic acid inactivating solution into a microfluidic channel of the device by pumping; and then flowing a wash solution into the channel by pumping, thereby displacing the nucleic acid inactivating solution from the channel, whereby any residual nucleic acid from a prior use of the device is inactivated.

Abstract translation: 提供了一种用于制备适用于核酸分析重用的微流体装置的方法。 该方法可以包括通过泵送将核酸灭活溶液流动到装置的微流体通道中; 然后通过泵送将洗涤溶液流入通道，​​从而使来自通道的核酸灭活溶液移位，由此来自先前使用该装置的任何残留核酸被灭活。