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    Synthetically regulated region
    12.
    发明专利
    Synthetically regulated region 失效
    综合监管区域

    公开(公告)号：JPS6158591A

    公开(公告)日：1986-03-25

    申请号：JP18114985

    申请日：1985-08-20

    Applicant: Hoechst Ag

    Inventor： YOOAHIMU ENGERUSU ,  OIGEEN UURUMAN ,  MIHIAERU RAINEBUEEBAA

    IPC: C12N15/09 ,  C07K14/005 ,  C07K14/195 ,  C07K14/52 ,  C07K14/555 ,  C07K14/57 ,  C12N1/20 ,  C12N15/72 ,  C12P21/02 ,  C12R1/19

    CPC classification number: C12N15/72 ,  C07K14/57

    Abstract: 1. Claims (for the Contracting States : BE, CH, DE, FR, GB, IT, LI, LU, NL and SE) A synthetic regulation region for the expression of heterologous genes in E. coli, containing a promotor, a modified lac operator and a ribosome-binding site, characterized in that a) the -35 region in the promotor has the nucleotide sequence (coding strand) TTGACAT or CTTGACAT b) the -10 region in the promotor has the nucleotide sequence (coding strand) GTATAAT, c) a spacer group of 14 to 17 base-pairs is between the -35 and the -10 regions, and d) a spacer group of 6 to 14 base-pairs is located between the ribosome-binding site and the ATG start codon. 1. Claims (for the Contracting State AT) A process for the expression of heterologous genes in E. coli, characterized by placing, upstream of the heterologous gene in the direction of reading, a synthetic regulation region which contains a promotor, a modified lac operator and a ribosome-binding site, in which a) the -35 region in the promotor has the nucleotide sequence (coding strand) TTGACAT or CTTGACAT b) the -10 region in the promotor has the nucleotide sequence (coding strand) GTATAAT, c) a spacer group of 14 to 17 base-pairs is between the -35 and the -10 regions, and d) a spacer group of 6 to 14 base-pairs is located between the ribosome-binding site and the ATG start codon.

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