公开(公告)号：EP4165641A1

公开(公告)日：2023-04-19

申请号：EP21739206.7

申请日：2021-06-10

Applicant: Life Technologies Corporation

Inventor： CHU, Yong ,  SCHNEIDER, Stephanie, Jo ,  SCHAEFFER, Rylan ,  WOO, David

IPC: G16B40/10 ,  G16B30/00 ,  G06N3/02

公开(公告)号：EP3844677A1

公开(公告)日：2021-07-07

申请号：EP19769625.5

申请日：2019-08-29

Applicant: Life Technologies Corporation

Inventor： CHU, Yong ,  MAJUMBAR, Nivedita ,  ALIMINATI, Manjula ,  CHEN, Yongzhi ,  PAPENFUSS, Kevin

IPC: G06N3/04 ,  G06N3/08 ,  G01N35/00 ,  G01N35/02 ,  G06T7/00

公开(公告)号：EP3253492A2

公开(公告)日：2017-12-13

申请号：EP16708007.6

申请日：2016-02-05

Applicant: Life Technologies Corporation

Inventor： CHU, Yong ,  MARKS, Jeffrey ,  FREUDENTHAL, Jacob ,  CHEN, Mingsong ,  TOH, Tiong Han ,  AGUANNO, Mauro ,  LAU, Lik Seng ,  LOH, Lian Seng ,  TEO, Kok Siong ,  CHU, Zeng Wei ,  MATHERS, Xin ,  UY, Michael ,  YEO, Huei Steven ,  BOO, Kuan Moon ,  LEE, Way Xuang ,  KOO, Chin Yong ,  TEO, Wei Fuh ,  LAU, Soo Yong ,  SHIN, Hon Siu ,  TAN, Zeqi ,  WESSEL, Thomas ,  WOO, David

IPC: B01L7/00 ,  G01N21/64 ,  G01N35/02 ,  G01N35/00

CPC classification number: B01L7/52 ,  B01L2200/145 ,  B01L2300/18 ,  B01L2300/1822 ,  C12Q1/6806 ,  G01N21/274 ,  G01N21/6452 ,  G01N21/6486 ,  G01N35/026 ,  G01N2021/6439 ,  G01N2035/00316 ,  G01N2035/00366 ,  G01N2201/061 ,  G01N2201/12

Abstract: A biological analysis system is provided. The system comprises a sample block assembly. The sample block assembly comprises a sample block configured to accommodate a sample holder, the sample holder configured to receive a plurality of samples. The system also comprises a control system configured to cycle the plurality of samples through a series of temperatures. The system further comprises an automated tray comprising a slide assembly, the tray configured to reversibly slide the sample block assembly from a closed to an open position to allow user access to the plurality of sample holders.

公开(公告)号：EP4379392A3

公开(公告)日：2024-08-21

申请号：EP24157142.1

申请日：2016-02-05

Applicant: Life Technologies Corporation

Inventor： CHU, Yong ,  MARKS, Jeffrey ,  FREUDENTHAL, Jacob ,  CHEN, Mingsong ,  TOH, Tiong Han ,  AGUANNO, Mauro ,  LAU, Lik Seng ,  LOH, Lian Seng ,  TEO, Kok Siong ,  CHU, Zeng Wei ,  MATHERS, Xin ,  UY, Michael ,  YEO, Huei Steven ,  BOO, Kuan Moon ,  LEE, Way Xuang ,  KOO, Chin Yong ,  TEO, Wei Fuh ,  LAU, Soo Yong ,  SHIN, Hon Siu ,  TAN, Zeqi ,  WESSEL, Thomas ,  WOO, David

IPC: B01L7/00 ,  G01N21/64 ,  G01N35/02 ,  G01N35/00 ,  G01N21/27

CPC classification number: G01N35/026 ,  G01N2035/0031620130101 ,  G01N2035/0036620130101 ,  G01N21/274 ,  G01N21/6452 ,  G01N2021/643920130101 ,  B01L2200/14520130101 ,  B01L2300/182220130101 ,  B01L7/52

Abstract: A biological analysis system is provided. The system comprises a sample block assembly. The sample block assembly comprises a sample block configured to accommodate a sample holder, the sample holder configured to receive a plurality of samples. The system also comprises a control system configured to cycle the plurality of samples through a series of temperatures. The system further comprises an automated tray comprising a slide assembly, the tray configured to reversibly slide the sample block assembly from a closed to an open position to allow user access to the plurality of sample holders.

公开(公告)号：EP3254080A1

公开(公告)日：2017-12-13

申请号：EP16706289.2

申请日：2016-02-05

Applicant: Life Technologies Corporation

Inventor： WESSEL, Thomas ,  CHU, Yong ,  FREUDENTHAL, Jacob ,  WOO, David

IPC: G01N21/27 ,  G01N21/64 ,  C12Q1/68 ,  G06F19/26

CPC classification number: G01N21/6428 ,  G01N21/274 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G06F19/24

Abstract: In one exemplary embodiment, a method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves. The validation plate includes a sample of a first quantity and a second quantity, and each amplification curve includes an exponential region. The method further includes determining a set of fluorescence thresholds based on the exponential regions of the plurality of amplification curves and determining, for each fluorescence threshold of the set, a first set of cycle threshold (Ct) values of amplification curves generated from the samples of the first quantity and a second set of Ct values of amplification curves generated from the samples of the second quantity. The method includes calculating if the first and second quantities are sufficiently distinguishable based on Ct values at each of the plurality of fluorescence thresholds.

公开(公告)号：EP2976434B1

公开(公告)日：2017-08-02

申请号：EP14722900.9

申请日：2014-03-18

Applicant: Life Technologies Corporation

Inventor： CHU, Yong ,  MARKS, Jeffrey

IPC: C12Q1/68 ,  G06K9/00 ,  G06T5/00

CPC classification number: G06T7/74 ,  C12Q1/686 ,  G06K9/00 ,  G06K2209/07 ,  G06T5/002 ,  G06T7/0012 ,  G06T7/11 ,  G06T7/136 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20024 ,  G06T2207/20208 ,  G06T2207/30072

Abstract: A method for analyzing biological reaction systems is provided. The method includes receiving an image of a substrate including a plurality of reaction sites after a biological reaction has taken place. Next, the method includes removing a noise background from the first image. The method includes determining an initial position of each reaction site based on an intensity threshold to generate a initial position set, then refining the initial position set of each reaction site based on an expected pattern of locations of the plurality of reaction sites to generate a first refined position set. The method further includes determining a presence or absence of a fluorescent emission from each reaction site based on the first refined position set and the first image.

Abstract translation: 提供了一种分析生物反应系统的方法。 该方法包括在生物反应发生后接收包括多个反应位点的底物的图像。 接下来，该方法包括从第一图像中去除噪声背景。 该方法包括基于强度阈值确定每个反应位点的初始位置以产生初始位置集合，然后基于多个反应位点的预期位置模式精炼每个反应位点的初始位置集合以产生第一 精致的位置设置。 该方法还包括基于第一改进位置集合和第一图像来确定来自每个反应位点的荧光发射的存在或不存在。

公开(公告)号：EP2976434A1

公开(公告)日：2016-01-27

申请号：EP14722900.9

申请日：2014-03-18

Applicant: Life Technologies Corporation

Inventor： CHU, Yong ,  MARKS, Jeffrey

IPC: C12Q1/68 ,  G06K9/00 ,  G06T5/00

CPC classification number: G06T7/74 ,  C12Q1/686 ,  G06K9/00 ,  G06K2209/07 ,  G06T5/002 ,  G06T7/0012 ,  G06T7/11 ,  G06T7/136 ,  G06T7/194 ,  G06T2207/10064 ,  G06T2207/20024 ,  G06T2207/20208 ,  G06T2207/30072

Abstract: A method for analyzing biological reaction systems is provided. The method includes receiving an image of a substrate including a plurality of reaction sites after a biological reaction has taken place. Next, the method includes removing a noise background from the first image. The method includes determining an initial position of each reaction site based on an intensity threshold to generate a initial position set, then refining the initial position set of each reaction site based on an expected pattern of locations of the plurality of reaction sites to generate a first refined position set. The method further includes determining a presence or absence of a fluorescent emission from each reaction site based on the first refined position set and the first image.

Abstract translation: 提供了分析生物反应体系的方法。 该方法包括在发生生物反应之后接收包括多个反应位点的底物的图像。 接下来，该方法包括从第一图像中去除噪声背景。 该方法包括基于强度阈值确定每个反应位点的初始位置以产生初始位置集，然后基于多个反应位点的预期位置模式来精化每个反应位点的初始位置集合，以产生第一 精致定位。 该方法还包括基于第一精制位置集和第一图像确定来自每个反应位点的荧光发射的存在或不存在。

公开(公告)号：EP4494150A1

公开(公告)日：2025-01-22

申请号：EP23714426.6

申请日：2023-03-15

Applicant: Life Technologies Corporation

Inventor： CHU, Yong ,  CHANDA, Deepankar ,  MAJUMDAR, Nivedita, Sumi ,  GEORGE, Wallace ,  JIANG, Ming ,  GAUTAM, Anurag ,  WONG, Nicolas Zun Yu ,  ZHU, Yun ,  SHAPIRO, Avi, Moshe ,  MOZHAYSKIY, Vadim ,  PATIL, Jasmine ,  PATEL, Dhvani, Pratik

IPC: G16B40/10 ,  G16B40/20

公开(公告)号：EP3895171A1

公开(公告)日：2021-10-20

申请号：EP19832518.5

申请日：2019-12-10

Applicant: Life Technologies Corporation

Inventor： CHU, Yong ,  SCHNEIDER, Stephanie ,  SCHAEFFER, Rylan ,  WOO, David

IPC: G16B30/20 ,  G16B40/20

公开(公告)号：EP3254083A1

公开(公告)日：2017-12-13

申请号：EP16708006.8

申请日：2016-02-05

Applicant: Life Technologies Corporation

Inventor： CHU, Yong ,  FREUDENTHAL, Jacob ,  MARKS, Jeffrey

IPC: G01N21/27 ,  G01N21/64 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/127

Abstract: Some embodiments describe a computer-implemented method for calibrating a fluorescent dye. The method can comprise imaging a sample holder, loaded into an instrument, at more than one channel. The sample holder can comprise a plurality of reaction sites and more than one dye type, with each dye occupying more than one reaction site. The method can further comprise identifying a peak channel for each dye on the sample holder, normalizing each channel to the peak channel for each dye, and producing a dye matrix that can comprise a set of dye reference values.