公开(公告)号：EP2785860B1

公开(公告)日：2015-06-03

申请号：EP12781556.1

申请日：2012-10-29

Applicant: Life Technologies Corporation

Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2537/16

公开(公告)号：EP3495497A1

公开(公告)日：2019-06-12

申请号：EP18194509.8

申请日：2012-04-27

Applicant: Life Technologies Corporation

Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

IPC: C12Q1/68

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

公开(公告)号：EP3180445A1

公开(公告)日：2017-06-21

申请号：EP15753859.6

申请日：2015-08-14

Applicant: Life Technologies Corporation

Inventor： BRAMLETT, Kelli ,  LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

IPC: C12Q1/68

CPC classification number: C12Q1/6846 ,  C12Q1/6855 ,  C12Q2525/117 ,  C12Q2535/10 ,  C12Q2537/143 ,  C12Q2525/191 ,  C12Q2563/185

Abstract: In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.

Abstract translation: 在一些实施方案中，本公开内容一般涉及包含多重核酸扩增反应的方法，组合物，系统，装置和试剂盒，所述多重核酸扩增反应使用多个（例如数百，数千，数万或数百） 目标特异性引物对，其能够基本上同时扩增单个反应混合物中的多个不同的目标靶序列。 在一些实施方案中，多重核酸扩增反应产生多个扩增子，所述扩增子具有来自含有RNA或DNA的样品的序列，包括全转录组或基因组样品。 在一些实施方案中，通过合适的检测方法，包括测序或本领域已知的其他程序，任选地同时或通过单个测定来表征多个扩增子中的至少一些的序列和丰度。

公开(公告)号：EP3072977A1

公开(公告)日：2016-09-28

申请号：EP15200283.8

申请日：2012-04-27

Applicant: Life Technologies Corporation

Inventor： LEAMON, John ,  ANDERSEN, Mark ,  THORNTON, Michael

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

Abstract translation: 本发明提供了可用于样品中存在的一种或多种核酸的多重PCR的方法，组合物，试剂盒，系统和装置。 特别地，提供允许选择性扩增一个或多个靶序列的各种靶特异性引物。 一方面，本发明涉及可用于选择性扩增与癌症或遗传性疾病相关的一种或多种靶序列的靶特异性引物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的扩增的靶序列可用于包括核酸测序在内的各种下游过程，并用于检测遗传变异体的存在。