公开(公告)号：EP3702383A1

公开(公告)日：2020-09-02

申请号：EP20150423.0

申请日：2016-07-01

Applicant: Life Technologies Corporation ,  Life Technologies AS

Inventor： FONNUM, Geir ,  MENCHEN, Steven M. ,  GOKMEN, Talha M. ,  LUNDBERG, Pontus ,  THWAR, Prasanna Krishnan ,  LUI, Alfred ,  LU, Lily ,  HINZ, Wolfgang ,  HUSABOE, Lene ,  BREIVOLD, Elisabeth ,  MOLTEBERG, Astrid Evenroed ,  LARSEN, Synne

IPC: C08F220/58 ,  C08F8/12

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：EP3320115A1

公开(公告)日：2018-05-16

申请号：EP16742101.5

申请日：2016-07-05

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven M. ,  XIANG, Guobing ,  THWAR, Prasanna Krishnan ,  FREDIANI, Jeffrey ,  WONG, Allen ,  LUI, Alfred ,  LU, Lily

IPC: C12Q1/68

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

公开(公告)号：EP3228715A3

公开(公告)日：2017-10-18

申请号：EP17160771.6

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

公开(公告)号：EP3317426B1

公开(公告)日：2020-01-15

申请号：EP16741745.0

申请日：2016-07-01

Applicant: Life Technologies Corporation ,  Life Technologies AS

Inventor： FONNUM, Geir ,  MENCHEN, Steven M. ,  LUI, Alfred ,  XIANG, Guobing ,  LARSEN, Synne

IPC: C12Q1/68 ,  G01N33/543

公开(公告)号：EP3228715B1

公开(公告)日：2019-08-14

申请号：EP17160771.6

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

公开(公告)号：EP3317314A1

公开(公告)日：2018-05-09

申请号：EP16741201.4

申请日：2016-07-01

Applicant: Life Technologies Corporation ,  Life Technologies AS

Inventor： FONNUM, Geir ,  MENCHEN, Steven, M. ,  GOKMEN, Talha, M. ,  LUNDBERG, Pontus ,  THWAR, Prasanna Krishnan ,  LUI, Alfred ,  LU, Lily ,  HINZ, Wolfgang ,  HUSABOE, Lene ,  BREIVOLD, Elisabeth ,  MOLTEBERG, Astrid, Evenroed ,  LARSEN, Synne

IPC: C08F220/58 ,  C08F8/12

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：EP2812446A1

公开(公告)日：2014-12-17

申请号：EP13705357.5

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.