公开(公告)号：EP2742151B1

公开(公告)日：2017-10-25

申请号：EP12751661.5

申请日：2012-08-10

Applicant: Life Technologies Corporation

Inventor： VANDER HORN, Peter ,  NIKIFOROV, Theo ,  LUO, Guobin ,  LANDES, Mindy ,  MAZUR, Daniel ,  TOZER, Eileen ,  LINCECUM, Tommie

IPC: C12Q1/68 ,  C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

公开(公告)号：EP3044329A1

公开(公告)日：2016-07-20

申请号：EP14777230.5

申请日：2014-09-12

Applicant: Life Technologies Corporation

Inventor： LUO, Guobin ,  SEDOVA, Marina ,  LIGHT, David ,  JONES, Ryan ,  ALANJARY, Mohammad

IPC: C12Q1/68

Abstract: A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.

Abstract translation: 对核酸链进行测序的方法包括接收具有与聚合物基质偶联的核酸链的颗粒，将颗粒暴露于包含缩合剂的溶液中，并将颗粒施加到表面上，颗粒沉积在表面上。

公开(公告)号：EP4282982A2

公开(公告)日：2023-11-29

申请号：EP23192384.8

申请日：2018-06-29

Applicant: Life Technologies Corporation

Inventor： ANDERSEN, Mark ,  MAZUR, Daniel ,  CHEN, Sihong ,  LUO, Guobin ,  PENG, Xinzhan

IPC: C12Q1/6853

Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.

公开(公告)号：EP3044329B1

公开(公告)日：2020-04-22

申请号：EP14777230.5

申请日：2014-09-12

Applicant: Life Technologies Corporation

Inventor： LUO, Guobin ,  SEDOVA, Marina ,  LIGHT, David ,  JONES, Ryan ,  ALANJARY, Mohammad

IPC: C12Q1/6806 ,  C12Q1/6874

公开(公告)号：EP3293274A3

公开(公告)日：2018-03-28

申请号：EP17198055.0

申请日：2012-08-10

Applicant: Life Technologies Corporation

Inventor： VANDER HORN, Peter ,  NIKIFOROV, Theo ,  LUO, Guobin ,  LANDES, Mindy ,  MAZUR, Daniel ,  TOZER, Eileen ,  LINCECUM, Tommie

IPC: C12Q1/68 ,  C12N9/12

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

Abstract translation: 本公开提供可用于核酸聚合的组合物，方法，试剂盒，系统和装置。 具体而言，提供了允许核酸扩增的修饰聚合酶及其生物活性片段。 在一个方面，本公开涉及用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式的遗传分析的修饰的聚合酶。 在一些方面，本公开涉及用于产生供各种下游过程使用的核酸文库或核酸模板的修饰聚合酶。 在一些方面，本公开涉及鉴定可以跨聚合酶的类或家族转移以提供具有改变的催化性质的新聚合酶的同源氨基酸突变。 在一些方面，本公开提供了与参考聚合酶相比具有增强的催化性质的修饰的聚合酶。

公开(公告)号：EP3293274A2

公开(公告)日：2018-03-14

申请号：EP17198055.0

申请日：2012-08-10

Applicant: Life Technologies Corporation

Inventor： VANDER HORN, Peter ,  NIKIFOROV, Theo ,  LUO, Guobin ,  LANDES, Mindy ,  MAZUR, Daniel ,  TOZER, Eileen ,  LINCECUM, Tommie

IPC: C12Q1/68 ,  C12N9/12

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.