公开(公告)号：EP3640254A1

公开(公告)日：2020-04-22

申请号：EP19201582.4

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： KJUS, Nini ,  FONNUM, Geir ,  BRANCHAUD, Bruce ,  YING, Lai-Qiang ,  MENCHEN, Steven ,  GREMYACHINSKIY, Dmitriy ,  KANG, Hee Chol

IPC: C07F7/18 ,  C08F222/38 ,  C08F220/58

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

公开(公告)号：EP3228715A2

公开(公告)日：2017-10-11

申请号：EP17160771.6

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

Abstract translation: 缀合的衬底的方法包括：交换与亲脂性抗衡离子的生物分子相关的抗衡离子，以形成生物分子复合物，在非水溶剂中的生物分子复合物分散，并在非水溶剂的存在下偶联，所述生物分子复合物到基底上 。 底物或固体支持物是聚合物颗粒，要固定在颗粒上的生物分子是寡核苷酸，亲脂性抗衡离子是四丁基铵离子，非水溶剂是N-甲基吡咯烷酮（NMP）。 寡核苷酸包被的颗粒用于核酸分离或核酸测序反应。

公开(公告)号：EP2812446B1

公开(公告)日：2017-05-10

申请号：EP13705357.5

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

Abstract translation: 缀合基质的方法包括将与生物分子相关的抗衡离子与亲脂性反离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在非水溶剂存在下将生物分子复合物偶联到底物 。

公开(公告)号：EP4185596A1

公开(公告)日：2023-05-31

申请号：EP21769210.2

申请日：2021-07-23

Applicant: Life Technologies Corporation

Inventor： BENSON, Scott ,  MULLAH, Khairuzzaman ,  CHANG, Chu-An ,  MENCHEN, Steven ,  LEE, Linda

IPC: C07H21/00 ,  C12Q1/6818

公开(公告)号：EP3626831A1

公开(公告)日：2020-03-25

申请号：EP19190463.0

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

公开(公告)号：EP2812339A1

公开(公告)日：2014-12-17

申请号：EP13705888.9

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： KJUS, Nini ,  FONNUM, Geir ,  BRANCHAUD, Bruce ,  YING, Lai-Qiang ,  MENCHEN, Steven ,  GREMYACHINSKIY, Dmitriy ,  KANG, Hee Chol

IPC: C07F7/18 ,  C08F222/38

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

Abstract translation: 描述了甲硅烷基保护的二丙烯酰胺化合物。 形成这种化合物的方法包括在第一反应条件下将甲硅烷基化试剂与羟基化二胺化合物混合以在第一溶液中形成产物，从第一溶液中分离产物，并将产物与丙烯酰氯在第二反应条件下在 第二种形成甲硅烷基保护的二丙烯酰胺化合物的溶液。

公开(公告)号：EP4116338A1

公开(公告)日：2023-01-11

申请号：EP22192503.5

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： FONNUM, Geir ,  MODAHL, Grete ,  KJUS, Nini ,  MOLTEBERG, Astrid Evenroed ,  TRAN, Diem ,  AASERUD, Jo ,  GOKMEN, M. Talha ,  MENCHEN, Steven ,  FULLER, Carl ,  ANDRUZZI, Luisa ,  HINZ, Wolfgang ,  GREMYACHINSKIY, Dmitriy ,  LIGHT, David ,  MASTROIANNI, Alexander ,  ROSENBLUM, Barnett ,  KHAN, Shaheer ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C08F2/16 ,  C08F220/54 ,  C08F8/12 ,  C08F222/38 ,  C12Q1/68 ,  C09D5/00 ,  C08F2/20 ,  C08F2/26 ,  C08F230/08 ,  C08F257/02 ,  C08F220/58 ,  C08F220/60 ,  C08F8/34 ,  C08F130/08 ,  C07F7/18

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：EP3228715A3

公开(公告)日：2017-10-18

申请号：EP17160771.6

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： MENCHEN, Steven ,  BLANCHARD, Alan ,  ANDRUZZI, Luisa ,  KHAN, Shaheer ,  GREMYACHINSKIY, Dmitriy ,  LUI, Alfred ,  STOLARCZYK, Craig ,  SOKOLSKY, Tanya

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.

公开(公告)号：EP4185858A2

公开(公告)日：2023-05-31

申请号：EP21755281.9

申请日：2021-07-23

Applicant: Life Technologies Corporation

Inventor： BENSON, Scott ,  MULLAH, Khairuzzaman Bashar ,  CHANG, Chu-An ,  MENCHEN, Steven ,  FREUDENTHAL, Jacob ,  LEE, Linda

IPC: G01N21/64 ,  C12Q1/68 ,  C12Q1/686

公开(公告)号：EP2812339B1

公开(公告)日：2019-11-06

申请号：EP13705888.9

申请日：2013-02-08

Applicant: Life Technologies Corporation

Inventor： KJUS, Nini ,  FONNUM, Geir ,  BRANCHAUD, Bruce ,  YING, Lai-Qiang ,  MENCHEN, Steven ,  GREMYACHINSKIY, Dmitriy ,  KANG, Hee Chol

IPC: C08F220/58 ,  C08F230/08 ,  C08F236/20 ,  C08K3/20 ,  C07F7/18 ,  C08F222/38