公开(公告)号：EP2699903B1

公开(公告)日：2018-07-18

申请号：EP12719172.4

申请日：2012-04-20

Applicant: Life Technologies Corporation

Inventor： REARICK, Todd ,  REED, Jessica ,  GIOIA, Jason ,  DRESSMAN, Devin ,  HAPSHE, Nicholas ,  REED, Brian

IPC: G01N33/50 ,  G01N33/543

CPC classification number: C12Q1/6874 ,  B01L3/5085 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/0893 ,  C12Q1/6837 ,  G01N33/50 ,  G01N33/543

Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.

公开(公告)号：EP2979083B1

公开(公告)日：2017-12-13

申请号：EP14722917.3

申请日：2014-03-28

Applicant: Life Technologies Corporation

Inventor： BALL, James, A. ,  REED, Brian

IPC: G01N27/414

CPC classification number: G01N27/4145 ,  B08B3/08 ,  B08B9/08 ,  C11D3/162 ,  C11D3/3418 ,  C11D3/43 ,  C11D7/34 ,  C11D7/5013 ,  C11D7/5022 ,  C11D7/5027

Abstract: A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.

公开(公告)号：EP3068902A1

公开(公告)日：2016-09-21

申请号：EP14821323.4

申请日：2014-11-07

Applicant: Life Technologies Corporation

Inventor： REED, Brian ,  BALL, James

IPC: C12Q1/68

CPC classification number: B01D17/047 ,  B01D17/04 ,  B01D17/041 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2563/159

Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

Abstract translation: 从乳液中回收珠粒载体的方法包括将表面活性剂水溶液供给到离心管中; 在离心管中的表面活性剂溶液上提供疏水性液体，其中表面活性剂水溶液的体积与疏水性液体的体积之比不大于0.5; 并且在离心时将乳液施加在疏水性液体上，所述乳液包含分散的水相，包括珠粒支持体，破乳剂和分散相的材料优先分配至表面活性剂溶液。

公开(公告)号：EP3428288A1

公开(公告)日：2019-01-16

申请号：EP18190894.8

申请日：2014-11-07

Applicant: Life Technologies Corporation

Inventor： REED, Brian ,  Ball, James

IPC: C12Q1/68 ,  C12Q1/6806

Abstract: A method of recovering a bead support from an emulsion is disclosed. The method comprises the following steps:
supplying an aqueous surfactant solution into a centrifuge tube; the surfactant solution including an anionic surfactant in a range of 5.0% to 15.0% and including a non-ionic surfactant in a range of 0.05% to 8.0%, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the centrifuge tube is not greater than 0.5 and at least 0.01; and
preparing an emulsion comprising a sample of template bead supports in a dispersed aqueous phase and a hydrophobic liquid in a continuous phase;
applying the emulsion into the centrifuge tube while centrifuging; and
recovering the sample of template bead supports from the surfactant solution.

公开(公告)号：EP3068902B1

公开(公告)日：2018-08-29

申请号：EP14821323.4

申请日：2014-11-07

Applicant: Life Technologies Corporation

Inventor： REED, Brian ,  BALL, James

IPC: C12Q1/6806

CPC classification number: B01D17/047 ,  B01D17/04 ,  B01D17/041 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2563/159

Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

公开(公告)号：EP3155127A1

公开(公告)日：2017-04-19

申请号：EP15731786.8

申请日：2015-06-11

Applicant: Life Technologies Corporation

Inventor： JOUN, David ,  LI, Chieh-Yuan ,  REED, Brian ,  OBERGFELL, Craig ,  DRESSMAN, Devin ,  ROSENBAUM, Abraham ,  BENSON, Scott ,  BROKA, Andi ,  GHOSH, Srinka

IPC: C12Q1/68

Abstract: In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads.

Abstract translation: 在一些实施方案中，本公开一般涉及组合物，其包含含有多个不同群体的离散支持物的单个反应混合物，以及多个不同的靶核酸群体。 单一反应混合物可以含有第一批珠粒; 第二颗珠子; 目标核酸的第一群体，其中靶核酸的第一群体中的至少两种不同靶核酸可以结合珠粒的第一群体中的珠粒; 和靶核酸的第二群体，其中靶核酸的第二群体中的至少两种不同靶核酸可以结合第二群珠体中的珠粒。 单个反应混合物可以用于单层放大第一珠上的第一靶核酸，并单核扩增第二珠上的第二靶核酸。

公开(公告)号：EP2699903A2

公开(公告)日：2014-02-26

申请号：EP12719172.4

申请日：2012-04-20

Applicant: Life Technologies Corporation

Inventor： REARICK, Todd ,  REED, Jessica ,  GIOIA, Jason ,  DRESSMAN, Devin ,  HAPSHE, Nicholas ,  REED, Brian

IPC: G01N33/50 ,  G01N33/543

CPC classification number: C12Q1/6874 ,  B01L3/5085 ,  B01L2200/0642 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/0893 ,  C12Q1/6837 ,  G01N33/50 ,  G01N33/543

Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.

Abstract translation: 提供了用于将样品沉积到表面上的方法，组合物，系统，装置和试剂盒。 样品可以包括一个或多个颗粒，并且表面可以包括一个或多个反应室。 在一些实施方案中，沉积可以包括使用伴随颗粒与样品颗粒的组合。