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    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME
    12.
    发明公开
    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME 有权
    改良蛋白酶自身VERFAHREN ZU IHRER HERSTELLUNG UND VERWENDUNG

    公开(公告)号：EP3040424A1

    公开(公告)日：2016-07-06

    申请号：EP15192848.8

    申请日：2011-02-28

    Applicant: Life Technologies Corporation

    Inventor： DAVIDSON, John F. ,  HINZ, Wolfgang ,  ROTHBERG, Jonathan ,  WHITAKER, Richard

    IPC: C12Q1/68 ,  C12Q1/48 ,  C12N9/12

    CPC classification number: C12N9/1252

    Abstract: Methods, compositions, systems, and kits comprising modified proteins, particularly modified nucleic acid-binding proteins and DNA polymerases with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

    Abstract translation: 提供了包含修饰的蛋白质，特别是经修饰的核酸结合蛋白质和具有改变的缓冲性质的DNA聚合酶的方法，组合物，系统和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰以获得所需pKa值的修饰蛋白质的方法。 此外，本发明提供了在离子产生反应中使用这种修饰的蛋白质的方法，例如基于离子的核酸测序反应。

    NUCLEIC ACID AMPLIFICATION
    13.
    发明公开
    NUCLEIC ACID AMPLIFICATION 有权
    核酸扩增

    公开(公告)号：EP2895620A1

    公开(公告)日：2015-07-22

    申请号：EP13771260.0

    申请日：2013-09-10

    Applicant: Life Technologies Corporation

    Inventor： LI, Chieh-Yuan ,  RUFF, David ,  CHEN, Shiaw-Min ,  O'NEIL, Jennifer ,  KASINSKAS, Rachel ,  ROTHBERG, Jonathan ,  HINZ, Wolfgang

    IPC: C12Q1/68

    CPC classification number: C12Q1/6846 ,  C12Q2521/101 ,  C12Q2521/507 ,  C12Q2522/101 ,  C12Q2527/101

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Abstract translation: 在一些实施方案中，本教导提供了用于核酸扩增的方法，包括形成反应混合物，并使反应混合物经历适于核酸扩增的条件。 在一些实施方案中，用于核酸扩增的方法包括使待扩增的核酸经历部分变性条件。 在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性扩增的核酸。 在一些实施方案中，用于核酸扩增的方法使用催化同源重组和聚合酶的酶。 在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。 在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单一连续液相中进行，而不需要反应混合物的区室化或反应组分的固定化。 在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选地在聚合物存在下将至少一种多核苷酸扩增到表面上。 聚合物可以包含筛分剂和/或扩散减少剂。

    NUCLEIC ACID AMPLIFICATION
    17.
    发明公开
    NUCLEIC ACID AMPLIFICATION 有权
    核酸扩增

    公开(公告)号：EP2839026A1

    公开(公告)日：2015-02-25

    申请号：EP13713677.6

    申请日：2013-03-15

    Applicant: Life Technologies Corporation

    Inventor： LI, Chieh-Yuan ,  RUFF, David ,  CHEN, Shiaw-Min ,  O'NEIL, Jennifer ,  KASINSKAS, Rachel ,  ROTHBERG, Jonathan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q2521/507 ,  C12Q2527/101 ,  C12Q2527/153 ,  C12Q2535/122 ,  C12Q2563/155 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/607

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming an reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of an reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    NUCLEIC ACID AMPLIFICATION
    19.
    发明公开
    NUCLEIC ACID AMPLIFICATION 审中-公开
    核酸扩增

    公开(公告)号：EP3095879A1

    公开(公告)日：2016-11-23

    申请号：EP16177421.1

    申请日：2013-03-15

    Applicant: Life Technologies Corporation

    Inventor： LI, Chieh-Yuan ,  RUFF, David ,  CHEN, Shiaw-Min ,  O'NEIL, Jennifer ,  KASINSKAS, Rachel ,  ROTHBERG, Jonathan

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  C12Q2521/507 ,  C12Q2527/101 ,  C12Q2527/153 ,  C12Q2535/122 ,  C12Q2563/155 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2565/607

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming an reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of an reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Abstract translation: 在一些实施方案中，本教导提供了用于核酸扩增的方法，包括形成反应混合物，并使反应混合物经受适于核酸扩增的条件。 在一些实施方案中，用于核酸扩增的方法包括使待扩增的核酸经历部分变性条件。 在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性扩增的核酸。 在一些实施方案中，用于核酸扩增的方法使用催化同源重组和聚合酶的酶。 在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。 在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单一连续液相中进行，而不需要反应混合物的区室化或反应组分的固定化。 在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选地在聚合物存在下将至少一种多核苷酸扩增到表面上。 聚合物可以包含筛分剂和/或扩散减少剂。

    NUCLEIC ACID AMPLIFICATION
    20.
    发明公开
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：EP3461910A1

    公开(公告)日：2019-04-03

    申请号：EP18194742.5

    申请日：2013-03-15

    Applicant: Life Technologies Corporation

    Inventor： LI, Chieh-Yuan ,  RUFF, David ,  CHEN, Shiaw-Min ,  O'NEIL, Jennifer ,  KASINSKAS, Rachel ,  ROTHBERG, Jonathan

    IPC: C12Q1/68

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming an reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of an reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

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