公开(公告)号：EP3293274A2

公开(公告)日：2018-03-14

申请号：EP17198055.0

申请日：2012-08-10

Applicant: Life Technologies Corporation

Inventor： VANDER HORN, Peter ,  NIKIFOROV, Theo ,  LUO, Guobin ,  LANDES, Mindy ,  MAZUR, Daniel ,  TOZER, Eileen ,  LINCECUM, Tommie

IPC: C12Q1/68 ,  C12N9/12

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

公开(公告)号：EP4461821A3

公开(公告)日：2025-04-09

申请号：EP24200607.0

申请日：2015-12-16

Applicant: Life Technologies Corporation

Inventor： MAZUR, Daniel ,  TOZER, Eileen ,  CHEN, Sihong ,  VANDER HORN, Peter ,  LINCECUM, Tommie

IPC: C12N9/12 ,  C12Q1/68 ,  C12Q1/686 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

公开(公告)号：EP4461821A2

公开(公告)日：2024-11-13

申请号：EP24200607.0

申请日：2015-12-16

Applicant: Life Technologies Corporation

Inventor： MAZUR, Daniel ,  TOZER, Eileen ,  CHEN, Sihong ,  VANDER HORN, Peter ,  LINCECUM, Tommie

IPC: C12Q1/686

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

公开(公告)号：EP3795681A2

公开(公告)日：2021-03-24

申请号：EP20186874.2

申请日：2015-12-16

Applicant: Life Technologies Corporation

Inventor： MAZUR, Daniel ,  TOZER, Eileen ,  CHEN, Sihong ,  VANDER HORN, Peter ,  LINCECUM, Tommie

IPC: C12N9/12 ,  C12Q1/68 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

公开(公告)号：EP3293274B1

公开(公告)日：2020-04-01

申请号：EP17198055.0

申请日：2012-08-10

Applicant: Life Technologies Corporation

Inventor： VANDER HORN, Peter ,  NIKIFOROV, Theo ,  LUO, Guobin ,  LANDES, Mindy ,  MAZUR, Daniel ,  TOZER, Eileen ,  LINCECUM, Tommie

IPC: C12Q1/686 ,  C12N9/12

公开(公告)号：EP4219739A2

公开(公告)日：2023-08-02

申请号：EP22208638.1

申请日：2014-09-30

Applicant: Life Technologies Corporation

Inventor： VANDER HORN, Peter ,  MAZUR, Daniel ,  NIKIFOROV, Theo ,  LANDES, Mindy ,  TOZER, Eileen

IPC: C12Q1/68 ,  C12N9/12

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

公开(公告)号：EP4141126A1

公开(公告)日：2023-03-01

申请号：EP22185494.6

申请日：2016-09-27

Applicant: Life Technologies Corporation

Inventor： MAZUR, Daniel ,  VANDER HORN, Peter ,  TOZER, Eileen ,  CHEN, Sihong ,  LUO, Guobin ,  SHIRLEY, Joshua ,  HEINEMANN, Kevin

IPC: C12Q1/68 ,  C12N9/12

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.

公开(公告)号：EP3234115A2

公开(公告)日：2017-10-25

申请号：EP15823870.9

申请日：2015-12-16

Applicant: Life Technologies Corporation

Inventor： MAZUR, Daniel ,  TOZER, Eileen ,  CHEN, Sihong ,  VANDER HORN, Peter ,  LINCECUM, Tommie

IPC: C12N9/12 ,  C12Q1/68

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a reference Taq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.

Abstract translation: 本公开提供可用于核酸聚合的组合物，方法，试剂盒，系统和装置。 具体而言，提供了修饰的聚合酶及其生物活性片段，例如修饰的Taq聚合酶，其允许改善的核酸扩增。 在一些方面，本公开内容提供了与参照Taq聚合酶相比具有改善的热稳定性，准确性，持续合成能力和/或阅读长度的修饰的聚合酶。 在一些方面，本公开涉及可用于扩增方法的修饰聚合酶或其生物活性片段，并且在实际示例性实施方案中为乳液PCR。

公开(公告)号：EP3052652A1

公开(公告)日：2016-08-10

申请号：EP14790851.1

申请日：2014-09-30

Applicant: Life Technologies Corporation

Inventor： VANDER HORN, Peter ,  MAZUR, Daniel ,  NIKIFOROV, Theo ,  LANDES, Mindy ,  TOZER, Eileen

IPC: C12Q1/68 ,  C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/686 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

公开(公告)号：EP2742151A2

公开(公告)日：2014-06-18

申请号：EP12751661.5

申请日：2012-08-10

Applicant: Life Technologies Corporation

Inventor： VANDER HORN, Peter ,  NIKIFOROV, Theo ,  LUO, Guobin ,  LANDES, Mindy ,  MAZUR, Daniel ,  TOZER, Eileen ,  LINCECUM, Tommie

IPC: C12Q1/68 ,  C12N9/12

CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/686 ,  C12Y207/07007

Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

Abstract translation: 本公开提供了可用于核酸聚合的组合物，方法，试剂盒，系统和装置。 特别地，提供了允许核酸扩增的修饰的聚合酶及其生物活性片段。 一方面，本公开涉及可用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式遗传分析的修饰聚合酶。 在一些方面，本公开涉及可用于产生用于各种下游过程的核酸文库或核酸模板的修饰聚合酶。 在一些方面，本公开涉及可以跨聚合酶类或家族转移的同源氨基酸突变的鉴定，以提供具有改变的催化性质的新型聚合酶。 在一些方面，本公开提供与参考聚合酶相比具有增强的催化性质的改性聚合酶。