公开(公告)号：JP2009122115A

公开(公告)日：2009-06-04

申请号：JP2009000168

申请日：2009-01-05

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  SHIMADA YOSHIHIRO

IPC: G01N21/64 ,  C12M1/34 ,  G01N33/48 ,  G01N33/483 ,  G06T1/00

Abstract: PROBLEM TO BE SOLVED: To provide a cell image analyzer that precisely extracts the total number of cells or the characteristic quantity of an individual cell. SOLUTION: The cell image analyzer is equipped with: a cell image acquiring means 55 for imaging a plurality of cells developed in color by different dyes under different conditions to acquire a plurality of images; and an image analyzing means 80 for extracting predetermined element contained in the respective cells in a plurality of images. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供精确提取单元的总数或单个单元的特征量的单元图像分析器。 解决方案：细胞图像分析仪装备有：细胞图像获取装置55，用于在不同条件下对由不同染料显色的多个细胞成像以获取多个图像; 以及用于提取包含在多个图像中的各个单元格中的预定元素的图像分析装置80。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2008157984A

公开(公告)日：2008-07-10

申请号：JP2006343118

申请日：2006-12-20

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： HARADA MITSUO ,  SHIMADA YOSHIHIRO

IPC: G02B21/00 ,  G01N21/64

CPC classification number: G02B21/16 ,  G02B21/0088 ,  G02B21/33 ,  G02B21/367

Abstract: PROBLEM TO BE SOLVED: To provide a fluorescent imaging method and a fluorescent imaging apparatus by which an excellent fluorescence image can be obtained by maintaining an excellent liquid pouring state without forcing an observer to continuously monitor the liquid pouring state over a long term in long-term observation such as time lapse observation. SOLUTION: The fluorescent imaging method, in which an inverted fluorescence imaging apparatus provided with an immersion objective is used to image fluorescent observation object for a fixed term, includes: a step S1 of locating a preset well in a vessel having a plurality of wells storing the fluorescent observation object at the imaging position of the fluorescent imaging apparatus; a liquid pouring step S2 of pouring a preset amount of liquid between the top portion of the immersion objective in the fluorescent imaging apparatus and the preset well; an imaging step S3 of imaging the fluorescent observation object in the preset well in a state where a space between the top portion of the immersion objective and the preset well is filled with the liquid; and a control step S4 of repeating a series of operations including the liquid pouring step S2 of a single operation and the imaging step S3 of a preset number of times of operations performed after the liquid pouring step S2 of the single operation, at preset time intervals. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 解决的问题：提供一种荧光成像方法和荧光成像装置，通过该荧光成像方法和荧光成像装置，通过保持优异的液体倾倒状态而不迫使观察者长期连续监测液体倾倒状态，可以获得优异的荧光图像 在长时间观察中，如时间间隔观察。 解决方案：使用设置有浸没物镜的反相荧光成像设备来定影荧光观察对象的荧光成像方法包括：将预定井定位在具有多个 存储荧光观察对象的荧光成像装置的成像位置的孔; 在所述荧光成像装置中的所述浸没物体的顶部与所述预设井之间注入预定量的液体的液体倾倒步骤S2; 在浸没物体的顶部与预设井的空间被液体填充的状态下对预定井中的荧光观察对象进行成像的成像步骤S3; 以及控制步骤S4，重复包括单次操作的液体注入步骤S2和在单次操作的液体浇注步骤S2之后执行的预设次数的操作的成像步骤S3的一系列操作，以预设的时间间隔 。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2008139820A

公开(公告)日：2008-06-19

申请号：JP2007139328

申请日：2007-05-25

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TSUTSUI AKIRA ,  SHIMADA YOSHIHIRO

IPC: G02B21/06 ,  G02B19/00

Abstract: PROBLEM TO BE SOLVED: To provide a microscope illumination apparatus configured so that the loss of the amount of light is kept to a minimum and the light can be efficiently supplied to the microscope body, even in the case of light to be emitted, in particular, from any of at least three kinds of light sources, each of a different emission wavelength region and a different figure and size of the light emitting section, including a light source using an ultraviolet wavelength region as the emission wavelength. SOLUTION: The microscope illumination apparatus includes: at least three kinds of light sources 21 to 24, each of a different emission wavelength region and a different figure and size of the light-emitting section; collimator lenses 25 to 28; a means 29 introducing light emitted from the collimator lenses into a common optical path; an imaging lens 30 placed on the common optical path; and an optical fiber 31 placed so that the entrance end face 31a of the optical fiber is located at a position where the images of the individual light sources are formed through the imaging lens. The collimator lenses are constructed to have different optical properties so that an angle of incidence of light on the optical fiber through the imaging lens may become smaller than a critical angle of the optical fiber and each of images of the light sources formed through the imaging lens has the maximum size to such an extent that the image is not larger than the entrance end face of the optical fiber. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：为了提供一种显微镜照明装置，其配置为使光量的损失保持最小，并且能够将光有效地供给到显微镜主体，即使在发光的情况下 特别是至少三种光源中的任何一种，发光部分的不同发射波长区域和不同的图形和大小，包括使用紫外线波长区域作为发射波长的光源。 解决方案：显微镜照明装置包括：至少三种光源21至24，各发光波长区域和发光部分的尺寸和尺寸不同; 准直透镜25至28; 将准直透镜发射的光引入公共光路的装置29; 放置在公共光路上的成像透镜30; 以及光纤31，使得光纤的入射端面31a位于通过摄像透镜形成各个光源的图像的位置。 准直透镜被构造成具有不同的光学特性，使得通过成像透镜的光纤上的入射角可能变得小于光纤的临界角以及通过成像透镜形成的每个光源的图像 具有图像不大于光纤的入射端面的程度的最大尺寸。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2005227098A

公开(公告)日：2005-08-25

申请号：JP2004035343

申请日：2004-02-12

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： YAMAGUCHI MITSUSHIRO ,  SHIMADA YOSHIHIRO

IPC: G01N21/64 ,  G02B21/00

Abstract: PROBLEM TO BE SOLVED: To provide an analyzer for detecting the presence of liquid penetration, and to provide its program.
SOLUTION: The analyzer is equipped with a light source (1), a sample tank (17) for housing a sample, a lens (3) for condensing the light from the light source toward the sample tank, a photodetector (14) for detecting the reflected light from the sample tank and a medium determining means (20) for determining whether the gap between the lens and the sample tank is filled with a medium for enhancing optical dissolving power on the basis of the output signal of the photodetector.
COPYRIGHT: (C)2005,JPO&NCIPI

Abstract translation: 要解决的问题：提供一种用于检测液体渗透性的分析仪，并提供其程序。 解决方案：分析仪配备有光源（1），用于容纳样品的样品槽（17），用于将来自光源的光聚焦到样品池的透镜（3），光电检测器（14） ），用于检测来自样品槽的反射光;以及介质确定装置（20），用于基于光电检测器的输出信号来确定透镜和样品箱之间的间隙是否填充有用于增强光溶解功率的介质 。 版权所有（C）2005，JPO＆NCIPI

公开(公告)号：JP2005062687A

公开(公告)日：2005-03-10

申请号：JP2003295400

申请日：2003-08-19

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： SHIMADA YOSHIHIRO

IPC: G02B21/24

Abstract: PROBLEM TO BE SOLVED: To provide a liquid supplying and discharging device for an immersion objective lens which do not exert adverse influence on aiming accuracy, whose reliability is high and whose maintainability is good.
SOLUTION: The liquid discharging device for the immersion objective lens is equipped with 1st liquid receiving parts (3 and 3a) attached to the immersion objective lens (2) and receiving liquid overflowing from the edge of the immersion objective lens and dropping it downward by gravity, a 2nd liquid receiving part (4) receiving the liquid dropped from the 1st liquid receiving parts by gravity, and a discharged liquid storage means (8) located under the 2nd liquid receiving part and storing the liquid discharged from the 2nd liquid receiving part.
COPYRIGHT: (C)2005,JPO&NCIPI

Abstract translation: 要解决的问题：提供一种浸没物镜的液体供给和排出装置，其不会对可靠性高且可维护​​性良好的瞄准精度产生不利影响。 解决方案：浸没式物镜的液体排放装置配备有安装在浸没物镜（2）上的第一液体接收部分（3和3a），并接收从浸没物镜的边缘溢出并将其滴落的液体 通过重力下降的第二液体接收部分（4），接收从第一液体接收部分重力下落的液体的第二液体接收部分（4）和位于第二液体接收部分下面的排出液体存储装置（8），并且储存从第二液体 接收部分。 版权所有（C）2005，JPO＆NCIPI

公开(公告)号：JP2005062105A

公开(公告)日：2005-03-10

申请号：JP2003295399

申请日：2003-08-19

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： YAMAGUCHI MITSUSHIRO ,  SHIMADA YOSHIHIRO ,  NANBA AKIHIRO ,  FUKUOKA TAKAHISA

IPC: G01N21/64

Abstract: PROBLEM TO BE SOLVED: To provide a photodetection device and a photodetection method, capable of simply and inexpensively coping with the changes in a medium temperature, without impeding measuring work on a test sample.
SOLUTION: This photodetection device has a holding means 4 for holding the test sample and a known sample, a light source means 2 for irradiating the test sample and the known sample with light, a photodetection means 12 for detecting light from the test sample and the known sample, a means 13 for holding the relation between the physical properties and the temperature of the known sample acquired beforehand, and a means 13 for performing the correction operation of the physical properties of the test sample, relative to the temperature from the relation between the physical property and the temperature of the known sample.
COPYRIGHT: (C)2005,JPO&NCIPI

Abstract translation: 要解决的问题：提供能够简单且低成本地应对中等温度的变化的光检测装置和光电检测方法，而不会妨碍测试样品的测量作业。 解决方案：该光检测装置具有用于保持测试样品和已知样品的保持装置4，用于用光照射测试样品和已知样品的光源装置2，用于检测来自测试的光的光检测装置12 样品和已知样品，用于保持预先获取的已知样品的物理性质和温度之间的关系的装置13以及用于执行测试样品的物理性质的校正操作的装置13相对于来自 物理性质与已知样品的温度之间的关系。 版权所有（C）2005，JPO＆NCIPI

公开(公告)号：JP2009151023A

公开(公告)日：2009-07-09

申请号：JP2007327789

申请日：2007-12-19

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TSUTSUI AKIRA ,  MATSUO YUICHIRO ,  HARADA MITSUO ,  SHIMADA YOSHIHIRO

IPC: G02B21/06 ,  F21V9/40 ,  G01N21/64 ,  H01L33/00 ,  H01L33/50 ,  H01L33/58 ,  H01L33/60

CPC classification number: G01N21/6428 ,  A61B5/0059 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6484 ,  G01N2201/0624 ,  G01N2201/0627 ,  G02B21/06

Abstract: PROBLEM TO BE SOLVED: To provide an illumination apparatus for the cellular analysis apparatus in which the loss of light is kept to a minimum, the amount of light of the LED light sources is automatically maintained constant, and changes of the location, shape, and intensity of fluorescent light of a substance for its object can be quantitatively obtained. SOLUTION: The illumination apparatus for the cellular analysis apparatus includes: a plurality of LEDs 11R, 11G, 11B of different center emission wavelengths; a photodetector 12; a path sharing means for introducing light emitted from the plurality of LEDs into the common optical path; a light-introducing device located on the common optical path to introduce part of the light emitted from the plurality of LEDs passing through the common optical path into the photodetector; a feedback controller 15 controlling turning-on states of the LEDs over preset states in accordance with the amount of light emitted from the plurality of LEDs detected by the photodetector; and an illumination light supplying device 16 which is located on the common optical path and supplies light which is emitted from the plurality of LEDs to pass through the common optical path and is not introduced into the photodetector through the light-introducing device, as illumination light for a cellular analysis. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：为了提供将光损失保持在最小值的细胞分析装置的照明装置，LED光源的光量自动保持恒定，并且位置的变化， 可以定量地获得物体的荧光的形状和强度。 解决方案：用于细胞分析装置的照明装置包括：具有不同中心发射波长的多个LED 11R，11G，11B; 光电检测器12; 用于将从所述多个LED发射的光引入所述公共光路中的路径共享装置; 位于公共光路上的光引入装置，用于将从通过公共光路的多个LED发射的光的一部分引入光电检测器; 反馈控制器15，根据由光检测器检测到的多个LED发出的光量，控制LED的预置状态的接通状态; 以及照明光供给装置16，其位于公共光路上，并且供给从多个LED射出的光，以通过公共光路，并且不通过光导入装置引入到光检测器中作为照明光 用于细胞分析。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009064398A

公开(公告)日：2009-03-26

申请号：JP2007234077

申请日：2007-09-10

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： TAKAGI KOSUKE ,  MATSUO YUICHIRO ,  SHIMADA YOSHIHIRO

IPC: G06T1/00 ,  G01N33/48

Abstract: PROBLEM TO BE SOLVED: To provide a cell analysis method, apparatus and program that can increase the accuracy of evaluation of drug reactions of cells.
SOLUTION: The cell analysis method includes an imaging step of imaging each cell group cultured under a different condition, a luminance measurement step of measuring the luminance of each cell from the cell image acquired in the imaging step, a distribution characteristic creation step of creating distribution characteristics of the luminance data under each condition, and a normalization step of normalizing the distribution characteristics created under each condition.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供可以提高细胞药物反应评价的准确性的细胞分析方法，装置和程序。 解决方案：细胞分析方法包括对在不同条件下培养的每个细胞群进行成像的成像步骤，从在成像步骤中获取的细胞图像测量每个细胞的亮度的亮度测量步骤，分布特征产生步骤 在每个条件下产生亮度数据的分布特性，以及规范化在每个条件下产生的分布特性的归一化步骤。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009003093A

公开(公告)日：2009-01-08

申请号：JP2007162590

申请日：2007-06-20

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： HARADA MITSUO ,  SHIMADA YOSHIHIRO

IPC: G02B21/24

CPC classification number: G02B21/26 ,  G02B21/0088 ,  G02B21/362

Abstract: PROBLEM TO BE SOLVED: To provide a box type motor-driven microscope which achieves miniaturization of an entire device by suppressing the operation range of an opening/closing member for changing a sample container to the utmost, which accurately maintains observing and measuring accuracy by suppressing wear in opening/closing operation, which excels in operability and safety, and additionally, for which observing and measuring conditions are easily changed, and further which achieves instantaneous observation and measurement of the change of the sample by manual injection of a reagent and confirmation of the operation circumstances inside the microscope.
SOLUTION: The box type motor-driven microscope includes: a motor-driven microscope part 10 having a transmissive illumination optical system 11, a motor-driven stage 12 and an image-formation optical system 14; and a housing 20. The housing has a fixed housing 21 and a movable housing 22, and the movable housing can parallel displace in an oblique direction relative to the fixed housing while holding an optical element above the motor-driven stage, so as to change the sample container 40 placed on the motor-driven stage, hermetically seal the motor-driven microscope part and shield it from light in cooperation with the fixed housing, and also nearly match the optical axis of the transmissive illumination optical system and the optical axis of the image-formation optical system.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供一种盒式电动显微镜，其通过抑制用于更换样品容器的开闭部件的操作范围来实现整个装置的小型化，从而精确地维持观察和测量 通过抑制打开/关闭操作中的磨损的精度，其操作性和安全性优异，另外，观察和测量条件容易改变，并且进一步通过手动注射试剂来实现对样品的变化的瞬时观察和测量 并确认显微镜下的操作情况。 盒式电动显微镜包括：具有透射照明光学系统11的电动显微镜部分10，电机驱动台12和成像光学系统14; 壳体20具有固定壳体21和可动壳体22，并且可动壳体能够相对于固定壳体在倾斜方向上平行移动，同时将光学元件保持在电动驱动平台上方，以便改变 放置在马达驱动平台上的样本容器40与电动驱动显微镜部分密封地密封，并与固定壳体协调地遮蔽光，并且几乎与透射照明光学系统的光轴和光轴 图像形成光学系统。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2008224992A

公开(公告)日：2008-09-25

申请号：JP2007062423

申请日：2007-03-12

Applicant: Olympus Corp ,  オリンパス株式会社

Inventor： HARADA MITSUO ,  SHIMADA YOSHIHIRO

IPC: G02B7/04 ,  G02B7/16 ,  G02B21/02 ,  G02B21/32

CPC classification number: G02B27/0068 ,  G02B21/0088 ,  G02B21/241 ,  G02B21/248

Abstract: PROBLEM TO BE SOLVED: To provide a corrector ring driving device of a microscopic objective lens provided with a corrector ring in which the exchange of a corrector ring mounting objective lens and the introduction of the objective lens into the observation optical path can be facilitated even in a state where the corrector ring mounting objective lens is mounted to a revolver, the working load of a viewer is lessened, and the corrector ring can be automatically driven. SOLUTION: A device driving the corrector ring 4a of the corrector ring mounting objective lens 4 mounted to an objective mounting and dismounting section 3a of the revolver 3 includes a rotation driving mechanism 1 having a motor 1a and a torque transmitting section 1b which transmits a torque of the motor 1a to the corrector ring 4a of the corrector ring mounting objective lens 4 mounted to the revolver 3 to drive the corrector ring 4a; and a connection means 2 for connecting the torque transmitting section 1b to the corrector ring 4a of the corrector ring mounting objective lens 4 introduced into an observation optical path in association with a switching operation performed by the revolver 3 and disconnecting the torque transmitting section 1b from the corrector ring 4a of the corrector ring mounting objective lens 4 removed from the observation optical path. COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 要解决的问题：为了提供一种具有校正环的微观物镜的校正环驱动装置，其中校正器环安装物镜的更换和物镜在观察光路中的引入可以是 即使在校正器环安装物镜安装到旋转枪的状态下，观察者的工作负荷减轻，并且校正环可以被自动驱动。 解决方案：驱动安装在旋转枪3的目标安装和拆卸部分3a上的校正器环安装物镜4的校正环4a的装置包括具有马达1a和转矩传递部分1b的旋转驱动机构1， 将马达1a的转矩传递到安装在转轮3上的校正器环安装物镜4的校正环4a，驱动校正环4a; 以及连接装置2，用于将转矩传递部分1b连接到引导到观察光路中的校正器环安装物镜4的校正环4a，与由转轮3执行的切换操作相关联，并将扭矩传递部分1b从 校正器环安装物镜4的校正环4a从观察光路去除。 版权所有（C）2008，JPO＆INPIT