-
公开(公告)号：US11795493B2
公开(公告)日：2023-10-24
申请号：US17092379
申请日：2020-11-09
Applicant: RHEONIX, INC.
Inventor： Peng Zhou
IPC: B01L3/00 , C12Q1/6806 , C12N15/10 , C07H1/08
CPC classification number: C12Q1/6806 , B01L3/502715 , C07H1/08 , C12N15/1003 , B01L2200/0631 , C12Q1/6806 , C12Q2527/101 , C12Q2565/629
Abstract: A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process.
-
公开(公告)号：US10538805B2
公开(公告)日：2020-01-21
申请号：US14983913
申请日：2015-12-30
Applicant: Rheonix, Inc.
Inventor： Gwendolyn Spizz , Peng Zhou
IPC: C12Q1/68 , C12Q1/6851 , C12Q1/689 , C12Q1/70 , C12Q1/6806
Abstract: Methods and systems for detecting a target nucleic acid using the quantitative capabilities of real-time nucleic acid amplification systems and the multiplexing capabilities of hybridization systems, comprising: identifying a conservative sequence and a distinctive sequence within each target nucleic acid sequence; simultaneously amplifying the conservative region and the distinctive region; monitoring the amplification of the conservative region in real-time; identifying the distinctive region amplicon via multiplexed identification; and performing quantitative multiplexing analysis of the target by combining the real-time monitoring information with the multiplexed identification of the target nucleic acid.
-
公开(公告)号：US20160138099A1
公开(公告)日：2016-05-19
申请号：US14983913
申请日：2015-12-30
Applicant: Rheonix, Inc.
Inventor： Gwendolyn Spizz , Peng Zhou
IPC: C12Q1/68
CPC classification number: C12Q1/6851 , C12Q1/6806 , C12Q1/689 , C12Q1/707 , C12Q1/708 , C12Q2537/143 , C12Q2561/113 , C12Q2565/501
Abstract: Methods and systems for detecting a target nucleic acid using the quantitative capabilities of real-time nucleic acid amplification systems and the multiplexing capabilities of hybridization systems, comprising: identifying a conservative sequence and a distinctive sequence within each target nucleic acid sequence; simultaneously amplifying the conservative region and the distinctive region; monitoring the amplification of the conservative region in real-time; identifying the distinctive region amplicon via multiplexed identification; and performing quantitative multiplexing analysis of the target by combining the real-time monitoring information with the multiplexed identification of the target nucleic acid.
Abstract translation: 使用实时核酸扩增系统的定量能力和杂交系统的复用能力来检测靶核酸的方法和系统，包括：鉴定每个靶核酸序列内的保守序列和独特序列; 同时扩增保守区域和特征区域; 实时监控保守区域的放大; 通过复用识别识别特征区扩增子; 以及通过组合实时监测信息与目标核酸的复用鉴定来进行目标的定量多路复用分析。
-
公开(公告)号：US09328381B2
公开(公告)日：2016-05-03
申请号：US14798719
申请日：2015-07-14
Applicant: RHEONIX, INC.
Inventor： Peng Zhou , Lincoln C. Young , Benjamin Thomas , Zongyuan Chen , Todd Roswech , Gwendolyn Spizz , Rubina Yasmin , Greg Mouchka
CPC classification number: C12Q1/686 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L3/523 , B01L3/527 , B01L7/52 , B01L2200/10 , B01L2200/14 , B01L2300/0627 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/18 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , C12Q1/6809 , C12Q1/6813 , C12Q1/6825 , C12Q1/6837 , C12Q1/6846 , C12Q1/6876 , C12Q1/708 , C12Q2600/106 , C12Q2600/156 , C12Q2600/158 , G01N35/10 , G01N35/1002 , G01N2035/00346 , C12Q2565/629
Abstract: A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.
-
公开(公告)号：US09151701B2
公开(公告)日：2015-10-06
申请号：US14103008
申请日：2013-12-11
Applicant: RHEONIX, INC.
Inventor： Peng Zhou , Lincoln C. Young
CPC classification number: G01N1/28 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/00 , B01L2200/026 , B01L2200/027 , B01L2200/028 , B01L2200/04 , B01L2200/0621 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/0887 , B01L2300/1827 , B01L2400/0481 , B01L2400/0622 , B01L2400/0633 , B01L2400/0655 , B01L2400/0666 , B01L2400/084 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0059 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T436/117497 , Y10T436/2575
Abstract: The systems and methods disclosed herein include a microfluidic system, comprising a pneumatic manifold having a plurality of apertures, and a chip manifold having channels disposed therein for routing pneumatic signals from respective ones of the apertures to a plurality of valves in a microfluidic chip, wherein the channels route the pneumatic signals in accordance with a configuration of the plurality of valves in the microfluidic chip.
Abstract translation: 本文公开的系统和方法包括微流体系统，其包括具有多个孔的气动歧管和具有设置在其中的通道的芯片歧管，用于将来自相应孔的气动信号路由到微流体芯片中的多个阀，其中 通道根据微流体芯片中的多个阀的结构来传送气动信号。
-
Patent Agency Ranking
公开(公告)号：US20140377854A1
公开(公告)日：2014-12-25
申请号：US14478012
申请日：2014-09-05
Applicant: RHEONIX, INC.
Inventor： Peng Zhou
IPC: B01L3/00
Abstract: A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process.
Abstract translation: 微流体装置，方法和相关应用利用并应用于福尔马林固定的石蜡包埋（FFPE）组织样品，并且在核酸纯化步骤之前进行液 - 液萃取从组织样品中除去石蜡。 微流体装置包括专用液 - 液提取处理容器，核酸纯化工艺组分和核酸扩增反应器。 液 - 液萃取和核酸纯化试剂盒包括能够进行液 - 液萃取方法和核酸纯化方法的微流体装置，包括专用液 - 液萃取处理容器，不混溶液体或前体相 在容器中，核酸纯化方法组分，流式的核酸扩增反应器和适于实现液 - 液萃取方法和核酸纯化方法的试剂供应。
-
公开(公告)号：US20140322099A1
公开(公告)日：2014-10-30
申请号：US14268399
申请日：2014-05-02
Applicant: RHEONIX, INC.
Inventor： Peng Zhou , Lincoln C. Young
IPC: G01N1/28
CPC classification number: G01N1/28 , B01L3/5025 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/00 , B01L2200/026 , B01L2200/027 , B01L2200/028 , B01L2200/04 , B01L2200/0621 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/0887 , B01L2300/1827 , B01L2400/0481 , B01L2400/0622 , B01L2400/0633 , B01L2400/0655 , B01L2400/0666 , B01L2400/084 , F04B43/043 , F16K99/0001 , F16K99/0015 , F16K99/0059 , F16K2099/0084 , F16K2099/0094 , Y10T137/0324 , Y10T436/117497 , Y10T436/2575
Abstract: The systems and methods described herein include a microfluidic chip having a plurality of microfeatures interconnected to provide a configurable fluid transport system for processing at least one reagent. Inserts are provided to removably interfit into one or more of the microfeatures of the chip, wherein the inserts include sites for interactions with the reagent. As will be seen from the following description, the microfluidic chip and the inserts provide an efficient and accurate approach for conducting parallel assays.
Abstract translation: 本文所述的系统和方法包括具有互连以提供用于处理至少一种试剂的可配置流体输送系统的多个微特征的微流体芯片。 提供插入件可移除地与芯片的一个或多个微特征相配合，其中插入物包括与试剂相互作用的位点。 从以下描述可以看出，微流体芯片和插入物提供用于进行平行测定的有效和准确的方法。
-
公开(公告)号：US08852919B2
公开(公告)日：2014-10-07
申请号：US13678732
申请日：2012-11-16
Applicant: Rheonix, Inc.
Inventor： Peng Zhou
CPC classification number: C12Q1/6806 , B01L3/502715 , B01L2200/0631 , C07H1/08 , C12N15/1003 , C12Q2527/101 , C12Q2565/629
Abstract: A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process.
Abstract translation: 微流体装置，方法和相关应用利用并应用于福尔马林固定的石蜡包埋（FFPE）组织样品，并且在核酸纯化步骤之前进行液 - 液萃取从组织样品中除去石蜡。 微流体装置包括专用液 - 液提取处理容器，核酸纯化工艺组分和核酸扩增反应器。 液 - 液萃取和核酸纯化试剂盒包括能够进行液 - 液萃取方法和核酸纯化方法的微流体装置，包括专用液 - 液萃取处理容器，不混溶液体或前体相 在容器中，核酸纯化方法组分，流式的核酸扩增反应器和适于实现液 - 液萃取方法和核酸纯化方法的试剂供应。
-
公开(公告)号：US20140206009A1
公开(公告)日：2014-07-24
申请号：US14226204
申请日：2014-03-26
Applicant: RHEONIX, INC.
Inventor： Lincoln C. Young , Peng Zhou , Gwendolyn Spizz , Rubina Yasmin
CPC classification number: C12N15/1013 , C12Q1/6806
Abstract: A method is provided for purifying nucleic acid from a sample in a microfluidic device. The method can be used to purify nucleic acids from any source known in the art that comprises nucleic acids, such as prokaryotic or eukaryotic organisms, viruses, cell, tissues, organs, etc. In a specific example, the tissue is whole blood. The method for purifying nucleic acid may run fully automated in the microfluidic device.
Abstract translation: 提供了一种用于从微流体装置中的样品中纯化核酸的方法。 该方法可用于从本领域已知的任何来源纯化核酸，其包含核酸，例如原核或真核生物，病毒，细胞，组织，器官等。在具体实例中，组织是全血。 纯化核酸的方法可以在微流体装置中完全自动化。
-
公开(公告)号：US20220213883A1
公开(公告)日：2022-07-07
申请号：US17670051
申请日：2022-02-11
Applicant: Rheonix, Inc.
Inventor： Lincoln C. Young , Peng Zhou
Abstract: A channel-less microfluidic pump includes a cartridge including a substrate and an actuatable film layer disposed on the substrate, and a manifold having at least three actuatable void volumes separated by a plurality of wall sections and an actuatable flexible layer disposed on the manifold interfacing the actuatable film layer. In operation, the pump can be in an unactuated state wherein the actuatable film layer is disposed against the surface of the substrate or an actuated state wherein at least a portion of the flexible layer and a corresponding portion of the actuatable film layer are deflected into a corresponding void volume thus forming a fluidic volume between the deflected portion of the actuatable film layer and the surface of the substrate. In the actuated state, there is a fluidic gap between immediately adjacent void volumes formed by a thinned region of the flexible layer at a point of contact with a top surface of a wall section. A method of transporting fluid using the channel-less microfluidic pump is described.
-
-
-
-
-
-
-
-
-
Search Results
39
records
(took 0.038 seconds)
