Abstract:
The invention provides nuclease protection assay, as illustrated in the Figure, comprising: (A) attaching a nucleic acid probe comprising a first nucleotide sequence to a solid surface area; (B) contacting the nucleic acid probe with a nucleic acid template under conditions that promote hybridization between complementary polynucleotides, forming a probe-template complex if the template includes a segment that is complementary to the probe; (C) contacting the probe-template complex with a nuclease effective to selectively cleave the nucleotide bonds of (1) the first nucleotide sequence when the first nucleotide sequence is single stranded or (2) mismatched regions of the first nucleotide sequence when the first nucleotide sequence is in duplex nucleic acid; and (D) detecting the presence of duplex nucleic acids formed by the probe and template nucleic acids by detecting the presence of the first nucleotide sequence.
Abstract:
This invention is an inhaler providing surface topology (202, 204) for minimizing the area of contact between the medicament (107) and the surfaces of the inhaler. The surfaces are preferably made of a material having a low surface energy, and more preferably, also having, when uncharged, no substantial van der Waals or electrostatic interaction with the medicament. Furthermore, the material is preferably substantially chemically unreactive with the medicament.
Abstract:
A method and apparatus for performing bandedge equalization. Specifically, the apparatus contains a pre-equalizer (300, 302) for adjusting the amplitudes of the bandedges of a broadband signal in response to a control signal. A bandedge filter (312, 314) is connected to the pre-equalizer (300, 302) and extracts a bandedge signal from the broadband signal. Lastly, a bandedge signal processor (315) that is connected to the bandedge filter (312, 314) generates the control signal in response to said bandedge signal. In this manner, when the bandedges of the broadband signal are asymmetric, the apparatus adjusts the signal strength of each bandedge with respect to one another to equalize (balance) the bandedges. The balanced signal can then be used by a bandedge timing recovery circuit. As such, the accuracy of a bandedge timing recovery circuit is not impacted by the asymmetric bandedges of the input signal.
Abstract:
The invention provides a plate having a plurality of uniformly sized reaction cells formed in its upper surface, wherein the density of the reaction cells is at least about 10 cells per cm . Preferably, the area of each of the openings of the reaction cells is no more than about 55 % of the area defined by the multiplication product of (1) the pitch between reaction cells in separate rows and (2) the pitch between reaction cells in separate columns.
Abstract translation:本发明提供了一种具有在其上表面形成的多个均匀尺寸的反应池的板,其中反应池的密度为至少约10个细胞/ cm 2。 优选地,反应池的每个开口的面积不超过由(1)的乘积定义的面积的大约55%,单独的行中的反应单元之间的间距和(2)反应单元之间的间距 单独列。
Abstract:
A method and apparatus for assessing the visibility of differences between two input image sequences. The apparatus comprises a visual discrimination measure (112) having a retinal sampling section (330), a plurality of temporal filters (334, 335) and a spatial discrimination section (240). The retinal sampling section (330) applies a plurality of transformations to the input image sequences (310, 320) for simulating the image-processing properties of human vision. The temporal filters (334, 335) separate the sequences of retinal images into two temporal channels producing a lowpass temporal response and a bandpass temporal response. The spatial discrimination section (240) applies spatial processing to the temporal responses to produce an image metric (250) which is used to assess the visibility of differences between the two input image sequences.
Abstract:
A method and apparatus for adjusting encoding parameters in accordance with a perceptual metric derived from analyzing the contents of an image or video being coded (104). Namely, the perceptual metric is produced (112) by comparing an original image (102) (or image sequence) to a reconstructed image (110) (or image sequence) and processing the comparison results using a quantitative perceptual difference measure (112). The difference measure results in a perceptual metric representing the "fidelity" of the reconstructed image. The metric is used to control the encoding parameters (108) such that the coding of the image can be iteratively adjusted. Furthermore, the method and apparatus is used to provide an automated steerable image compression system.
Abstract:
A method and apparatus for processing a geometric relationship between the image motion of pairs of points over multiple image frames representing a three-dimensional scene. This relationship is based on the parallax motion of points with respect to an arbitrary planar surface, and does not involve epipolar geometry. A constraint is derived over two frames (504) for any pair of points (506), relating their projective structure (with respect to the plane) based only on their image coordinates and their parallax displacements. Similarly, a 3D-rigidity constraint between pairs of points over multiple frames is derived. Applications disclosed for these parallax-based constraints include recovery of 3D scene structure (512), detection of moving objects in the presence of camera induced motion (510), and synthesis of new camera views based on a given set of views (514). Moreover, the approach can handle difficult situations for 3D scene analysis, e.g., where there is only a small set of parallax vectors, and in the presence of independently moving objects.
Abstract:
The scene change detector compares predicted macroblocks from an anchor image (140) to input macroblocks from an input image (120) on a macroblock-by-macroblock basis to generate a residual macroblock (142) representing the difference between each predicted macroblock (P) and each input macroblock (I). A variance (146) for each residual macroblock and a variance (148) for each input macroblock is computed after each comparison. The residual variance is compared to the input macroblock variance. Whenever the variance of the residual macroblock exceeds the variance of the input macroblock, a counter (152) is incremented. The scene cut detector repeats this process until each macroblock in the predicted image is compared to each input macroblock. If the count value ever exceeds a threshold level (154) while an input image is being processed, the scene cut detector sets a scene cut indicator flag.
Abstract:
Patterned laminated green tape multilayer stacks (130) can be fired while maintaining the dimensions of the pattern (120) by applying, as by screen printing or spraying, a ceramic ink (108) over surface portions of the green tape stack. Complex patterns can be formed in the green tapes by punching openings (120) in one or more of the green tape stacks (100) before laminating them together.
Abstract:
A method of constructing an image mosaic comprising the steps of selecting source images, aligning the source images, selecting source segments, enhancing the images, and merging the images to form the image mosaic is disclosed. An apparatus for constructing an image mosaic comprising means for selecting source images (102), means for aligning the source images (103), means for selecting source image segments (104), means for enhancing the images (105), and means for merging (106) the images to form the image mosaic is also disclosed. The process may be performed automatically by the system or may be guided interactively by a human operator. Applications include the construction of photographic quality prints from video and digital camera images.