公开(公告)号：JP2009268385A

公开(公告)日：2009-11-19

申请号：JP2008120166

申请日：2008-05-02

Applicant: Sony Corp ,  ソニー株式会社

Inventor： ONISHI MICHIHIRO ,  KISHII NORIYUKI ,  KISHIMOTO TAKUYA ,  SASAKI NAOYUKI ,  WATANABE HIDETOSHI

IPC: C12M1/00 ,  C12N15/00

CPC classification number: C12N15/1006

Abstract: PROBLEM TO BE SOLVED: To provide a technique, in nucleic acid hybridization in a microflow path, for enabling high flow level of a solution to be accomplished through preventing the leakage of the injecting solution by preventing inner pressure rise.
SOLUTION: A method for producing a carrier for isolating a nucleic acid is provided, including the following procedure: A capturing chain 2 having a base sequence complementary to a target nucleic acid chain, with modified functional groups each having a positive electric charge, is brought to a solid phase by ion-exchange bonding on a porous carrier (microbeads) 1 having cation-exchangeable ion-exchange groups on the surface.
COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：为了提供一种技术，在微流路中的核酸杂交中，为了通过防止内部压力升高来防止注射溶液的泄漏，能够实现高流动度的溶液。 提供了一种用于分离核酸的载体的制备方法，包括以下步骤：具有与靶核酸链互补的碱基序列的捕获链2，具有正电荷的改性官能团 通过在表面具有可阳离子交换的离子交换基团的多孔载体（微珠）1上通过离子交换键合而成为固相。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2009198184A

公开(公告)日：2009-09-03

申请号：JP2008036836

申请日：2008-02-19

Applicant: Sony Corp ,  ソニー株式会社

Inventor： KISHIMOTO TAKUYA

IPC: G01N33/53 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N21/78 ,  G01N33/543

Abstract: PROBLEM TO BE SOLVED: To provide a fluorescent label microbead for nucleic acid detection for performing gene development detection in a live cell, and a method of acquiring information related to double-strand production using the fluorescent label microbead for nucleic acid detection.
SOLUTION: The fluorescent label microbead B
1 for nucleic acid detection includes, on its surface, oligonucleotide P having base sequence complementary to detecting object nucleic acid strand T, and an intercalative fluorochrome 1. In this method, by using the fluorescent label microbead B
1 for nucleic acid detection, and detecting information related to the fluorescence of the fluorescent label microbead B
1 for nucleic acid detection, information related to double-strand production of the fluorescent label microbead B
1 for nucleic acid detection and the detecting object nucleic acid strand T is obtained.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供用于在活细胞中进行基因发育检测的核酸检测用荧光标记微珠，以及使用荧光标记微珠进行核酸检测获得与双链生产有关的信息的方法。 解决方案：用于核酸检测的荧光标记微珠B 1 在其表面上包括具有与检测对象核酸链T互补的碱基序列的寡核苷酸P和插入式荧光染料1.在此 方法，通过使用荧光标记微珠B 1 进行核酸检测，并检测与荧光标记微珠B 1荧光相关的信息用于核酸检测，信息相关 用于核酸检测的荧光标记微珠B 1 和检测对象核酸链T的双链生产。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009189296A

公开(公告)日：2009-08-27

申请号：JP2008033164

申请日：2008-02-14

Applicant: Sony Corp ,  ソニー株式会社

Inventor： KISHIMOTO TAKUYA

IPC: C12Q1/68 ,  G01N21/78 ,  G01N33/58

CPC classification number: C12Q1/6818 ,  C12Q2565/1015 ,  C12Q2563/173

Abstract: PROBLEM TO BE SOLVED: To provide a fluorescent labeled oligonucleotide for nucleic acid detection to enable easy quantitative analysis of a gene with excellent analytic efficiency without necessitating the preparation of a calibration curve and to provide a method for getting information on double strand formation by using the fluorescent labeled oligonucleotide for nucleic acid detection.
SOLUTION: Provided is a fluorescent labeled oligonucleotide P
1 for nucleic acid detection having a base sequence complementary to a nucleic acid strand T for detection, wherein the fluorescent characteristic varies by the double strand formation with the nucleic acid strand T for detection. The fluorescent labeled oligonucleotide P
1 for nucleic acid detection can be produced by labeling with an intercalating fluorescent dye 1 and a non-intercalating fluorescent dye 2. Also provided is a method for getting information on the double strand formation by using the fluorescent labeled oligonucleotide P
1 for nucleic acid detection.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供用于核酸检测的荧光标记寡核苷酸，以便能够容易地定量分析具有优异分析效率的基因，而不需要制备校准曲线并提供获得双链形成信息的方法 通过使用荧光标记寡核苷酸进行核酸检测。 提供了用于核酸检测的荧光标记寡核苷酸P 1 ，其具有与用于检测的核酸链T互补的碱基序列，其中荧光特征随双链形成而变化 用于检测的核酸链T。 用于核酸检测的荧光标记寡核苷酸P 1 可以通过用插入式荧光染料1和非插层荧光染料2进行标记来制备。还提供了获得双链形成信息的方法 通过使用荧光标记寡核苷酸P 1 进行核酸检测。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009039065A

公开(公告)日：2009-02-26

申请号：JP2007209338

申请日：2007-08-10

Applicant: Sony Corp ,  ソニー株式会社

Inventor： ONISHI MICHIHIRO ,  KISHIMOTO TAKUYA ,  WATANABE HIDETOSHI ,  SEGAWA YUJI ,  SETORIYAMA TASUKU ,  ABE TOMOTERU ,  TAKEDA MINORU ,  SUGANUMA HIROSHI

IPC: C12Q1/04 ,  C12Q1/68 ,  G01N21/78 ,  G01N33/569

Abstract: PROBLEM TO BE SOLVED: To provide a virus detection method enabling real-time detection of the proliferation of viruses in a living cell without breaking the living cell.
SOLUTION: Viruses in a living cell are detected by the fluorescence detection of a fluorescent labeled nucleotide transferred into a virus genome. The fluorescent labeled nucleotide is labeled at least with a group of a plurality of fluorescent dyes containing an excitation object fluorescent dye to give an excitation energy acquired by light absorption to the other fluorescent dye, and a detection object fluorescent dye to emit light by receiving the excitation energy from the other fluorescent dye.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供能够实时检测活细胞中病毒增殖而不破坏活细胞的病毒检测方法。 解决方案：通过荧光标记的核苷酸转移到病毒基因组的荧光检测来检测活细胞中的病毒。 荧光标记的核苷酸至少用含有激发对象荧光染料的多种荧光染料的一组标记，得到通过光吸收获得的激发能量到另一种荧光染料，以及检测对象荧光染料通过接收 来自其他荧光染料的激发能。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2012152182A

公开(公告)日：2012-08-16

申请号：JP2011016212

申请日：2011-01-28

Applicant: Sony Corp ,  Tokyo Medical & Dental Univ ,  ソニー株式会社 ,  国立大学法人 東京医科歯科大学

Inventor： ONISHI MICHIHIRO ,  KISHIMOTO TAKUYA ,  WATANABE HIDETOSHI ,  MIZUTANI NAGANORI ,  TAKAGI MASATOSHI

IPC: C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/53 ,  G01N37/00

Abstract: PROBLEM TO BE SOLVED: To provide technologies for highly sensitively detecting a bcr-abl gene mRNA.SOLUTION: In the micro-channel 11 for nucleic acid hybridization, a sample solution containing the bcr-abl gene mRNA is circulated, and agarose gel carriers (agarose gel beads) 2 in which a capturing chain with a specific base sequence complementary to the bcr-abl gene mRNA is solid-phased are loaded in such a state as held by a filter 113 disposed on the downstream in the direction of feeding the sample solution.

Abstract translation: 要解决的问题：提供高灵敏度检测bcr-abl基因mRNA的技术。 解决方案：在用于核酸杂交的微通道11中，循环含有bcr-abl基因mRNA的样品溶液，以及琼脂糖凝胶载体（琼脂糖凝胶珠）2，其中具有特异碱基序列互补的捕获链 对bcr-abl基因的mRNA进行固相加载，其状态是由设置在供给样品溶液的方向的下游的过滤器113保持的状态。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2012018039A

公开(公告)日：2012-01-26

申请号：JP2010154678

申请日：2010-07-07

Applicant: Sony Corp ,  ソニー株式会社

Inventor： ONISHI MICHIHIRO ,  SASAKI NAOYUKI ,  KISHIMOTO TAKUYA ,  WATANABE HIDETOSHI

IPC: G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  G01N30/60 ,  G01N30/88 ,  G01N37/00

CPC classification number: C12Q1/6813 ,  C12Q2527/101 ,  C12Q2565/629

Abstract: PROBLEM TO BE SOLVED: To provide a technology for making accurate optical detection of a target nucleic acid by preventing blocking, reflection, and scattering of excited light and fluorescent light of a carrier, in a microchannel filled with a carrier for nucleic acid separation.SOLUTION: There is provided a microchannel 11 in which a solution containing the target nucleic acid chain flows, and which is filled with a agarose gel carrier (agarose gel beads) 2 having a solid phased capture chain which includes a base sequence complementary to the target nucleic acid chain in a state held by a filter 113 arranged at downstream side of flow direction of the solution.

Abstract translation: 要解决的问题：提供一种技术，用于通过在填充有核酸载体的微通道中防止载体的激发光和荧光的阻挡，反射和散射来进行目标核酸的精确光学检测 分离。 解决方案：提供了一种微通道11，其中含有靶核酸链的溶液流动，并且填充有具有固相捕获链的琼脂糖凝胶载体（琼脂糖凝胶珠）2，其包含互补的碱基序列 通过布置在溶液的流动方向的下游侧的过滤器113保持的状态下的目标核酸链。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2010088533A

公开(公告)日：2010-04-22

申请号：JP2008259135

申请日：2008-10-04

Applicant: Sony Corp ,  ソニー株式会社

Inventor： KISHIMOTO TAKUYA ,  ONISHI MICHIHIRO

IPC: A61N5/06

Abstract: PROBLEM TO BE SOLVED: To provide an apparatus and a method for stimulating neurocyte capable of stimulating to activate for a short time while downsizing more than ever before. SOLUTION: A pulse drive voltage is applied to a laser diode having a voltage value above a voltage for generating relaxation oscillation or more, and a laser light having a pulse-like specific peak is output from the laser diode, and the laser light having the pulse-like specific peak condenses on a stimulation subject in a sample. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供一种刺激神经细胞的装置和方法，该装置和方法能够在短时间内激发以在比以往更小的尺寸下激活。 解决方案：将脉冲驱动电压施加到具有高于用于产生弛豫振荡的电压的电压值的激光二极管以上，并且从激光二极管输出具有脉冲状特定峰值的激光，并且激光 具有脉冲状特定峰的光在样品中的刺激对象上冷凝。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2009207459A

公开(公告)日：2009-09-17

申请号：JP2008056342

申请日：2008-03-06

Applicant: Sony Corp ,  ソニー株式会社

Inventor： KISHIMOTO TAKUYA ,  WATANABE HIDETOSHI ,  ONISHI MICHIHIRO ,  SEGAWA YUJI ,  ABE TOMOTERU ,  TAKEDA MINORU ,  SUGANUMA HIROSHI

IPC: C12M1/00 ,  C12N15/09

Abstract: PROBLEM TO BE SOLVED: To provide a new apparatus for extracting a nucleic acid capable of extracting the nucleic acid from a trace amount of a sample without causing dilution of the nucleic acid due to no use of a chemical treatment and capable of rapidly carrying out the extraction of the nucleic acid because practice ranging from cytoclasis to even adsorption of the nucleic acid can be made in the simple apparatus.
SOLUTION: The apparatus for extracting the nucleic acid is designed to extract a specific nucleic acid from a biological sample. The apparatus for extracting the nucleic acid includes at least a flow channel in which the biological sample moves, a crushing section for crushing the biological sample in the flow channel, an enzyme inactivating section for inactivating an enzyme in the biological sample in the flow channel, and an adsorption carrier for adsorbing the extracted nucleic acid.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供一种新的装置，用于提取能够从微量样品中提取核酸的核酸，而不会由于不使用化学处理而导致核酸稀释，并能够快速 进行核酸的提取，因为在简单的装置中可以进行从细胞粘附到均匀吸附核酸的实践。 解决方案：用于提取核酸的装置被设计为从生物样品中提取特异性核酸。 用于提取核酸的装置至少包括生物样品移动的流路，用于粉碎流路中的生物样品的破碎部，用于使流路中的生物样品中的酶失活的酶灭活部， 和用于吸附提取的核酸的吸附载体。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009047540A

公开(公告)日：2009-03-05

申请号：JP2007213612

申请日：2007-08-20

Applicant: Sony Corp ,  ソニー株式会社

Inventor： KISHIMOTO TAKUYA ,  ONISHI MICHIHIRO ,  WATANABE HIDETOSHI

IPC: G01N21/75 ,  G01N21/64 ,  G01N33/68

Abstract: PROBLEM TO BE SOLVED: To provide a biosubstance detection method capable of measuring by a simpler operation in comparison with a conventional method, and detecting also a biosubstance in a living tissue or in a living cell.
SOLUTION: In this method, a biosubstance is allowed to progress a prescribed reaction by utilizing multiphoton excitation, and fluorescence from a self-fluorescent material which is a reaction product from the reaction is detected, to thereby detect the biosubstance. In addition, a method for determining a disease state and physiological function based on a fluorescence detection value acquired from the method is also provided.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供一种与常规方法相比能够通过更简单的操作进行测量并且还检测活体组织或活细胞中的生物活性的生物物质检测方法。 解决方案：在该方法中，通过利用多光子激发使生物体进行规定的反应，从检测到来自反应的反应产物的自身荧光物质的荧光检测生物体。 此外，还提供了一种基于从该方法获取的荧光检测值来确定疾病状态和生理功能的方法。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2008289437A

公开(公告)日：2008-12-04

申请号：JP2007139891

申请日：2007-05-28

Applicant: Sony Corp ,  ソニー株式会社

Inventor： KISHIMOTO TAKUYA ,  WATANABE HIDETOSHI ,  ONISHI MICHIHIRO

IPC: C12Q1/25 ,  C12M1/34

CPC classification number: A61B5/0059 ,  A61B5/0071 ,  A61B5/441

Abstract: PROBLEM TO BE SOLVED: To provide a method for measuring an enzyme activity, capable of measuring using simpler operation, as compared with conventional methods, and further is capable of measuring the enzyme activity of the enzymes inside living tissues and living cells.
SOLUTION: This method for measuring the enzyme activity for measuring the amount of a substrate metabolite formed by substrate metabolism with the enzyme, by detecting an irradiated wave formed by the multiphoton excitation process of the substrate or substrate metabolite is provided. Also the device for measuring the enzyme activity is provided, at least by equipping a laser light source 11 of a near-infrared femtosecond laser light inducing the multiphoton excitation process of the substrate or substrate metabolite; an irradiated wave-detecting part 31 for detecting the irradiated wave formed by the multiphoton excitation process of the substrate or substrate metabolite and an optical passage for introducing the near infrared femtosecond laser light to an enzyme-presenting site; and also introducing the irradiated waves to the irradiated wave-detecting part.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 待解决的问题：提供一种与常规方法相比能够使用更简单的操作测量酶活性的方法，并且还能够测量活组织和活细胞内的酶的酶活性。 解决方案：提供了通过检测由底物或底物代谢物的多光子激发过程形成的照射波，测量用酶测量由底物代谢形成的底物代谢物的量的酶活性的方法。 还提供了用于测量酶活性的装置，至少通过装备激发光源11的近红外飞秒激光来诱导基质或底物代谢物的多光子激发过程; 用于检测由基板或基板代谢物的多光子激发过程形成的照射波的照射波检测部31和用于将近红外飞秒激光引入酶介导位点的光通路; 并且还将辐射波引入到照射波检测部。 版权所有（C）2009，JPO＆INPIT