公开(公告)号：WO2008156471A1

公开(公告)日：2008-12-24

申请号：PCT/US2007/025357

申请日：2007-12-12

Applicant: STC.UNM ,  TIMMINS, Graham ,  DERETIC, Vojo, P.

Inventor： TIMMINS, Graham ,  DERETIC, Vojo, P.

IPC: A61B6/00 ,  C12Q1/58

CPC classification number: A61B5/0002 ,  A61B5/082 ,  A61B5/411 ,  A61B5/415 ,  A61B5/416 ,  A61B5/418 ,  A61K49/0004 ,  G01N33/5695

Abstract: This invention relates to a test for detecting a Mycobacterium tuberculosis (tuberculosis or TB) infection in a patient or subject, specifically a diagnostic test, including a breath test, whereby patients are provided a small dose of an isotopically labeled TB drug, Isoniazid (INH) orally or directly to the lungs of the patient or subject. If TB is present, a TB enzyme mycobacterial peroxidase KatG oxidizes the INH; and KatG specific metabolites, in particular, isotopically labeled nitric oxide (NO), nitrites, nitrates, carbon monoxide (CO) or carbon dioxide converted from carbon monoxide of INH cleavage are measured. Other embodiments relate to a diagnostic breath test for detecting TB utilizing isotopically labeled urea (preferably, carbon- 13 labeled urea), alone or in combination with isotopically labeled isoniazid (preferably, nitrogen- 15 labeled isoniazid), wherein M. tuberculosis organism, if present in the patient or subject's lungs (or other tissues), will metabolize the isotopically labeled urea to isotopically labeled carbon dioxide (CO2) such that a determination of the residence of M. tuberculosis, including residence of an isoniazid resistant strain of M. tuberculosis, may be made.

Abstract translation: 本发明涉及一种用于检测患者或受试者中结核分枝杆菌（结核病或结核病）感染的试验，特别是包括呼吸试验的诊断试验，其中向患者提供小剂量的同位素标记的结核病药物异烟肼（INH ）口服或直接给予患者或受试者的肺。 如果TB存在，TB酶分枝杆菌过氧化物酶KatG氧化INH; 并测定KatG特异性代谢物，特别是同位素标记的一氧化氮（NO），亚硝酸盐，硝酸盐，一氧化碳（CO）或由INH切割的一氧化碳转化的二氧化碳。 其他实施方案涉及用于使用同位素标记的尿素（优选碳-13标记的尿素）单独或与同位素标记的异烟肼（优选氮-15标记的异烟肼）组合来检测结核病的诊断性呼吸试验，其中结核分枝杆菌生物 存在于患者或受试者的肺部（或其他组织）中，将同位素标记的尿素代谢为同位素标记的二氧化碳（CO 2），从而确定结核分枝杆菌的住所，包括结核分枝杆菌的异烟肼抗性菌株的停留 ，可以做。