公开(公告)号：US08664700B2

公开(公告)日：2014-03-04

申请号：US13096173

申请日：2011-04-28

Applicant: June-young Lee ,  Su-hyeon Kim

Inventor： June-young Lee ,  Su-hyeon Kim

IPC: G01N27/414

CPC classification number: H01L51/0093 ,  B82Y10/00 ,  H01L27/28 ,  H01L27/281 ,  H01L51/0508

Abstract: A bio material receiving device includes a thin film transistor (“TFT”) including a drain electrode, and a nano well accommodating a bio material. The drain electrode includes the nano well. The TFT may be a bottom gate TFT or a top gate TFT. A nano well array may include a plurality of bio material receiving devices. In a method of operating the bio material receiving device, each of the bio material receiving devices may be individually selected in the nano well array. When the bio material is accommodated in the selected bio material receiving device, a voltage is applied so that another bio material is not accommodated.

Abstract translation: 生物材料接收装置包括包括漏电极的薄膜晶体管（“TFT”）和容纳生物材料的纳米孔。 漏电极包括纳米孔。 TFT可以是底栅极TFT或顶栅极TFT。 纳米孔阵列可以包括多个生物材料接收装置。 在生物材料接收装置的操作方法中，每个生物材料接收装置可以在纳米孔阵列中单独选择。 当生物材料容纳在所选择的生物材料接收装置中时，施加电压，使得不容纳另一生物材料。

公开(公告)号：US08645078B2

公开(公告)日：2014-02-04

申请号：US13303861

申请日：2011-11-23

Applicant: Tae-jin Ahn ,  Soyeon Ahn ,  Sung-ho Won ,  Su-hyeon Kim ,  Taesung Park ,  Sung-young Lee ,  Seung-yeoun Lee ,  Bora Yeon ,  Young-ho Park

Inventor： Tae-jin Ahn ,  Soyeon Ahn ,  Sung-ho Won ,  Su-hyeon Kim ,  Taesung Park ,  Sung-young Lee ,  Seung-yeoun Lee ,  Bora Yeon ,  Young-ho Park

IPC: G06F19/00 ,  G11C17/00 ,  G06F15/00

CPC classification number: G06F19/22

Abstract: A method of recovering a nucleic acid sequence using a probe map includes: aligning a probe onto a target sequence based on a result in which the probe is hybridized to the target sequence; determining a representative value representing each aligned position of the probe; and recovering a base sequence of the target sequence by using a probe map to which the determined representative values and base sequence information of the probe are mapped.

Abstract translation: 使用探针图回收核酸序列的方法包括：基于探针与靶序列杂交的结果将探针对准靶序列; 确定表示探针的每个对准位置的代表值; 以及通过使用映射了所述探测器的确定的代表值和碱基序列信息的探针图来恢复所述目标序列的碱基序列。

公开(公告)号：US08470570B2

公开(公告)日：2013-06-25

申请号：US11684393

申请日：2007-03-09

Applicant: Kui-hyun Kim ,  Byung-chul Kim ,  Su-hyeon Kim

Inventor： Kui-hyun Kim ,  Byung-chul Kim ,  Su-hyeon Kim

IPC: C12N13/00

CPC classification number: C40B60/14 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00369 ,  B01J2219/00371 ,  B01J2219/00385 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00574 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00655 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  B01J2219/00743 ,  B01L3/0268 ,  B01L2400/027 ,  B01L2400/043 ,  G01N2035/1034 ,  Y10T436/25

Abstract: An apparatus for printing a biomolecular droplet onto a substrate using an electric charge concentration effect includes; an electric field forming electrode including an accommodating area in which the biomolecular droplet including micro magnetic beads is accommodated and a nozzle formed on an end of the accommodating area through which the biomolecular droplet is discharged, a substrate disposed below the electric field forming electrode, including a grounded target surface onto which the biomolecular droplet discharged from the nozzle of the electric field forming electrode is deposited, a magnet disposed below the substrate which applies a magnetic force on the micro magnetic beads, and an open circuit type voltage applying unit electrically connected to the electric field forming electrode which applies a charge to the electric field forming electrode which generates an electrical force which causes the biomolecular droplet to be ejected onto the target surface of the substrate.

Abstract translation: 使用电荷浓度效应将生物分子液滴印刷到基板上的装置包括： 包括容纳区域的电场形成电极，其中容纳包括微磁珠的生物分子液滴和形成在所述容纳区域的生物分子液滴被排出的端部的喷嘴，设置在所述电场形成电极下方的衬底，包括 沉积从电场形成电极的喷嘴排出的生物分子滴的接地目标表面，设置在基板上的对微小磁珠施加磁力的磁体，以及开路型电压施加单元，电连接到 该电场形成电极向电场形成电极施加电荷，该电场产生使生物分子液滴喷射到基板的目标表面上的电力。

公开(公告)号：US07531138B2

公开(公告)日：2009-05-12

申请号：US11560890

申请日：2006-11-17

Applicant: Su-hyeon Kim ,  Jeong-gun Lee

Inventor： Su-hyeon Kim ,  Jeong-gun Lee

IPC: B01L3/00 ,  G01N1/00 ,  C12M1/00

CPC classification number: C12M47/06 ,  B01L3/5027 ,  C12N1/066 ,  C12N13/00 ,  G01N1/44 ,  Y10T137/2496 ,  Y10T436/25

Abstract: A cell lysis device for lysing cells or viruses, comprising a cell lysis tube having a sample inlet; a pump connected to the cell lysis tube for transferring a sample into the tube; a sealing unit for reversibly sealing a specific region of the tube; and a laser source for generating a laser is provided. Further, a method of lysing cells or viruses using the cell lysis device is provided. The method comprises introducing a sample containing cells or viruses and optionally magnetic beads to the cell lysis tube through the sample inlet; transferring the sample to a specific region in the cell lysis tube by means of the pump; temporarily sealing the region of the cell lysis tube where the sample is placed with the sealing unit; irradiating the sample with the laser; removing the sealing unit from the cell lysis tube; and discharging the sample from the cell lysis tube by means of the pump.

Abstract translation: 一种用于裂解细胞或病毒的细胞裂解装置，包括具有样品入口的细胞裂解管; 连接到细胞裂解管的泵，用于将样品转移到管中; 用于可逆地密封管的特定区域的密封单元; 并提供用于产生激光的激光源。 此外，提供了使用细胞裂解装置裂解细胞或病毒的方法。 该方法包括通过样品入口将含有细胞或病毒的样品和任选的磁珠引入细胞裂解管; 通过泵将样品转移到细胞裂解管中的特定区域; 用密封单元临时密封放置样品的细胞溶解管的区域; 用激光照射样品; 从细胞裂解管中取出密封单元; 并通过泵从细胞裂解管排出样品。

公开(公告)号：US08771949B2

公开(公告)日：2014-07-08

申请号：US13303813

申请日：2011-11-23

Applicant: Tae-jin Ahn ,  Su-hyeon Kim ,  Kyoung-gu Woo

Inventor： Tae-jin Ahn ,  Su-hyeon Kim ,  Kyoung-gu Woo

IPC: C12Q1/68

CPC classification number: G06F19/16

Abstract: An efficient and accurate method of identifying a target biomolecule in a sample by using target molecule-probe binding frequencies is disclosed.

Abstract translation: 公开了通过使用靶分子 - 探针结合频率来鉴定样品中的靶生物分子的有效和准确的方法。

公开(公告)号：US08137523B2

公开(公告)日：2012-03-20

申请号：US11754523

申请日：2007-05-29

Applicant: Yoon-kyoung Cho ,  Su-hyeon Kim ,  Chin-sung Park ,  Kyu-sang Lee ,  Jeong-gun Lee

Inventor： Yoon-kyoung Cho ,  Su-hyeon Kim ,  Chin-sung Park ,  Kyu-sang Lee ,  Jeong-gun Lee

IPC: G01N27/26 ,  G01N27/447

CPC classification number: B03C5/005 ,  B03C5/026 ,  B03C2201/26

Abstract: An apparatus separating a polarizable analyte using dielectrophoresis includes a vessel including a membrane having a plurality of nano- to micro-sized pores, the membrane disposed inside the vessel, electrodes generating spatially non-uniform electric fields in the nano- to micro-sized pores of the membrane when an AC voltage is applied to the electrodes, and a power source applying the AC voltage to the electrodes, wherein a sectional area of the pores varies along a depth of the pores. A method of separating a polarizable material uses the apparatus.

Abstract translation: 使用介电电泳分离可极化分析物的装置包括容器，其包括具有多个纳米至微米尺寸孔的膜，所述膜设置在容器内部，电极在纳米至微孔中产生空间上不均匀的电场 当向电极施加AC电压时，将膜施加到膜上，并且向电极施加AC电压的电源，其中孔的截面积沿着孔的深度而变化。 分离可极化材料的方法使用该装置。

公开(公告)号：US07600840B2

公开(公告)日：2009-10-13

申请号：US11503786

申请日：2006-08-14

Applicant: Su-hyeon Kim ,  Jeong-gun Lee ,  Jong-suk Chung ,  Hye-jung Cho ,  Nam Huh ,  In-seok Kang ,  Beom-seok Lee

Inventor： Su-hyeon Kim ,  Jeong-gun Lee ,  Jong-suk Chung ,  Hye-jung Cho ,  Nam Huh ,  In-seok Kang ,  Beom-seok Lee

IPC: B41J29/38

CPC classification number: B41J2/06 ,  B01J19/0046 ,  B01J2219/00364 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00693 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00743 ,  B41J2/04535 ,  B41J2/0456 ,  B41J2/04576

Abstract: A device for printing a droplet onto a substrate includes: a droplet generating member which is needle-shaped and comprises a receiving portion disposed vertically to receive a solution, and a discharge hole connected to the receiving portion and formed on a bottom of the receiving portion so that the solution can be discharged from the receiving portion; a substrate disposed below the droplet generating member, the substrate includes a target portion to which the droplet discharged from the discharge hole of the droplet generating member is dropped and attached; a voltage applier applying a voltage to the droplet so that the droplet can be dropped onto the target portion of the substrate; a volume measuring unit measuring the volume of the droplet; and a droplet control unit maintaining the volume of the droplet at a predetermined level based on the measured volume of the droplet.

Abstract translation: 用于将液滴印刷到基板上的装置包括：液滴产生构件，其为针状并且包括垂直设置以接收溶液的接收部分，以及连接到接收部分并形成在接收部分的底部上的排出孔 使得溶液可以从接收部分排出; 设置在所述液滴产生部件下方的基板，所述基板包括从所述液滴产生部件的排出孔排出的液滴滴落并附着到的目标部分; 电压施加器，向所述液滴施加电压，使得所述液滴能够落到所述基板的所述目标部分上; 体积测量单元，测量液滴的体积; 以及液滴控制单元，其基于所测量的液滴体积将液滴的体积保持在预定水平。

公开(公告)号：US20060183112A1

公开(公告)日：2006-08-17

申请号：US11335246

申请日：2006-01-19

Applicant: Jun-hong Min ,  Su-hyeon Kim ,  In-ho Lee ,  Kui-hyun Kim ,  Seung-yeon Yang

Inventor： Jun-hong Min ,  Su-hyeon Kim ,  In-ho Lee ,  Kui-hyun Kim ,  Seung-yeon Yang

IPC: C12Q1/70 ,  C12Q1/68

CPC classification number: G01N33/5438 ,  G01N33/48721

Abstract: Provided is a method of separating particles, the method comprising: forming a first chamber and a second chamber separated by an interface with a pore, wherein the first and second chambers have electrodes with different polarities; placing particles to which a target biomolecule is bound from particles to which the target biomolecule is not bound in the first chamber; applying a voltage which has the same polarity as that of the target biomolecule to the electrode of the first chamber, and a voltage which has an opposite charge to that of the target biomolecule to the electrode of the second chamber; and translocating only the particles to which the target biomolecule is bound from the first chamber to the second chamber through the pore. Conventionally, the size of a pore is used to separate biomolecules. However, effective separation is difficult to achieve because the manufacture of a pore with a diameter of less than 10 nm, small enough to separate biomolecule, is not easy. Therefore, signal separation and data analysis must be required. However, in the present method, physical movement induced by the charge of biomolecules is used to effectively separate the biomolecules, thus obtaining a high signal to noise ratio. As a result, additional data analysis is not required.

Abstract translation: 提供了一种分离颗粒的方法，该方法包括：形成由与孔的界面分隔开的第一室和第二室，其中第一和第二室具有不同极性的电极; 在第一室中放置靶生物分子所结合的颗粒与目标生物分子不结合的颗粒; 将具有与目标生物分子相同极性的电压施加到第一室的电极，以及将具有与靶生物分子相反的电荷的电压施加到第二室的电极; 并且仅将来自第一室的目标生物分子结合的颗粒通过孔转移到第二室。 通常，使用孔的大小来分离生物分子。 然而，由于直径小于10nm的孔的制造足够小以分离生物分子，所以难以实现有效的分离，这是不容易的。 因此，必须要求信号分离和数据分析。 然而，在本方法中，由生物分子的电荷引起的身体运动用于有效分离生物分子，从而获得高的信噪比。 因此，不需要额外的数据分析。

公开(公告)号：US08724105B2

公开(公告)日：2014-05-13

申请号：US13160854

申请日：2011-06-15

Applicant: June-young Lee ,  Hee-jeong Jeong ,  Seong-ho Cho ,  Su-hyeon Kim

Inventor： June-young Lee ,  Hee-jeong Jeong ,  Seong-ho Cho ,  Su-hyeon Kim

IPC: G01N1/10 ,  B82Y15/00 ,  B01L3/00

CPC classification number: G01N1/10 ,  B01L3/502707 ,  B32B37/065 ,  B32B37/12 ,  B32B38/06 ,  B32B2535/00 ,  B82Y15/00 ,  G01N21/554 ,  G01N21/645 ,  Y10S977/953 ,  Y10S977/957 ,  Y10S977/958

Abstract: A nano particle tracking device includes a channel structure. The channel structure of the nano particle tracking device includes a pair of microchannels in which a specimen including nano particles is accommodated and which face each other, at least one nano channel which is between the pair of microchannels, which connects the pair of microchannels to each other and through which the nano particles in the specimen are moved, and a nano grating below the nano channel and crossing the nano channel perpendicularly.

Abstract translation: 纳米粒子跟踪装置包括通道结构。 纳米颗粒跟踪装置的通道结构包括一对微通道，其中包含纳米颗粒的样品被容纳并且彼此面对，在一对微通道之间的至少一个纳米通道，将一对微通道连接到每个微通道 样品中的纳米颗粒通过其移动，并且纳米通道下方的纳米光栅并且垂直穿过纳米通道。

公开(公告)号：US20130122577A1

公开(公告)日：2013-05-16

申请号：US13577418

申请日：2011-02-07

Applicant: Joo-won Rhee ,  Su-hyeon Kim ,  Jeong-gun Lee ,  Mi-jeong Song

Inventor： Joo-won Rhee ,  Su-hyeon Kim ,  Jeong-gun Lee ,  Mi-jeong Song

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B82Y15/00 ,  G01N33/5308 ,  Y10S977/774 ,  Y10S977/92 ,  C12Q2522/101 ,  C12Q2563/107

Abstract: Provided are kits for determining a nucleotide sequence of a target nucleic acid, the kit including at least one sequence specific binding protein and a detectable tag. In accordance with a kit for determining a nucleotide sequence of a target nucleic acid according to one exemplary embodiment and a method and device for determining a nucleotide sequence of a target nucleic acid, the nucleotide sequence of the target nucleic acid may be more efficiently determined.

Abstract translation: 提供了用于确定靶核酸的核苷酸序列的试剂盒，该试剂盒包括至少一种序列特异性结合蛋白和可检测标签。 根据一个示例性实施方案的用于确定靶核酸的核苷酸序列的试剂盒以及用于确定靶核酸的核苷酸序列的方法和装置，可以更有效地确定靶核酸的核苷酸序列。