公开(公告)号：EP4405678A1

公开(公告)日：2024-07-31

申请号：EP22871235.2

申请日：2022-09-26

Applicant: University Health Network

Inventor： KESHAVJEE, Shafique ,  SAGE, Andrew ,  CYPEL, Marcelo ,  MARTINU, Tereza

IPC: G01N33/50 ,  G01N33/52

CPC classification number: G01N33/92 ,  G01N2800/2420130101 ,  G01N2800/5020130101 ,  G01N33/6869 ,  G01N2333/52120130101 ,  G01N2333/5420130101 ,  G01N2333/541220130101 ,  G01N2333/542120130101 ,  G01N2800/0620130101 ,  G01N33/6893 ,  G01N33/54387

公开(公告)号：EP3362785A1

公开(公告)日：2018-08-22

申请号：EP16854672.9

申请日：2016-10-14

Applicant: University Health Network (UHN) ,  The Governing Council of the University of Toronto

Inventor： KESHAVJEE, Shafique ,  LIU, Mingyao ,  SAGE, Andrew ,  SARGENT, Edward ,  KELLEY, Shana

IPC: G01N27/416 ,  G01N27/327 ,  G01N33/543 ,  G01N27/30

CPC classification number: G01N27/3276 ,  B82Y30/00 ,  G01N27/3278 ,  G01N33/5438 ,  G01N33/563 ,  G01N33/566 ,  G01N2800/56 ,  G01N2800/60

Abstract: There is described herein a method for the electrochemical quantification of a protein analyte in sample, comprising: providing one or more electrode(s), each comprising at least one peptide attached to its surface, the peptide being the protein or a fragment thereof; contacting the sample and electrode with an antibody in the presence of a redox reporter, wherein the antibody is capable of binding to each of the protein analyte and the peptide on the electrode; measuring an electrochemical signal generated by the redox reporter when a potential is applied; quantifying the protein analyte by comparing the electrochemical signal generated with a control, wherein the electrochemical signal is indirectly proportional to the amount of protein analyte in the sample.