公开(公告)号：WO1993017781A1

公开(公告)日：1993-09-16

申请号：PCT/US1993002384

申请日：1993-03-12

Applicant: WORCESTER POLYTECHNIC INSTITUTE

Inventor： WORCESTER POLYTECHNIC INSTITUTE ,  MA, Yi, Hua ,  XIANG, Shouhe

IPC: B01D71/02

CPC classification number: B01D67/0051 ,  B01D71/028 ,  B01D2323/08 ,  B01D2323/12 ,  B01J20/183 ,  B01J29/06 ,  B01J37/0215 ,  B01J37/0246 ,  B01J2229/64

Abstract: The present invention relates to a method of forming a zeolite membrane. The method includes forming an aqueous-based or an alcohol-based sol which can form a zeolite. The sol composition is then disposed on a porous support. The porous support and the sol composition are exposed to an atmosphere and to a temperature sufficent to cause the sol composition to hydrothermally crystallize, thereby forming the zeolite membrane.

公开(公告)号：WO1990007860A1

公开(公告)日：1990-07-26

申请号：PCT/US1989005819

申请日：1989-12-26

Applicant: WORCESTER POLYTECHNIC INSTITUTE

Inventor： WORCESTER POLYTECHNIC INSTITUTE ,  GIBSON, Daniel, G., III ,  HILLY, Joan, Bakunas

IPC: C12N05/00

CPC classification number: C12N5/0601 ,  C12N2531/00

Abstract: A method for producing horseshoe crab amebocytes in vitro by isolating amebocyte producing tissue from the gill flaps of horseshoe crabs, especially Limulus polyphemus. In contrast to all previous attempts to isolate and culture amebocytes from Limulus for the production of pyrogen-sensitive lysate, an effective method and means have been developed utilizing the discovery that the gill flaps are the source of the cells which differentiate into competent amebocytes. Once isolated, the tissue can be cultured long term in vitro on an artificial surface or as part of the gill flap leaflets, opened along one edge to allow access of the media to the developing amebocytes. Amebocytes are removed from the gill flaps by pulsing with Limulus serum, copper sulfate, detergent, or combinations thereof.