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公开(公告)号：US10322411B2
公开(公告)日：2019-06-18
申请号：US15150062
申请日：2016-05-09
Applicant: Fluidigm Corporation
Inventor： Naga Gopi Devaraju , Marc A. Unger
Abstract: A microfluidic system includes a substrate, a set of input ports coupled to the substrate, and a set of output ports coupled to the substrate. The microfluidic system also includes a microfluidic processing system coupled to the substrate and including a plurality of processing sites. The microfluidic processing system is coupled to the set of input ports and the set of output ports. The microfluidic system further includes one or more microfluidic logic devices coupled to the substrate and operable to control at least a portion of the microfluidic processing system.
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公开(公告)号：US10226770B2
公开(公告)日：2019-03-12
申请号：US14864491
申请日：2015-09-24
Applicant: Fluidigm Corporation
Inventor： Jake Kimball , Brandon Ripley , Gang Sun , Dominique Toppani , Myo Thu Maung
Abstract: A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.
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公开(公告)号：US10190163B2
公开(公告)日：2019-01-29
申请号：US15055252
申请日：2016-02-26
Applicant: Fluidigm Corporation
Inventor： Carolyn G. Conant , Tze Howe Charn , Jason A. A. West , Xiaohui Wang
IPC: C12Q1/68 , C12Q1/6874 , C12Q1/6844 , B01L3/00
Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.
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204.发明申请公开(公告)号：US20180306683A1
公开(公告)日：2018-10-25
申请号：US15925600
申请日：2018-03-19
Applicant: Fluidigm Corporation
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C. Norris , Dominique G. Toppani , Marc A. Unger , Jing Wang , Jason A.A. West
IPC: G01N1/28 , C12P19/34 , C12Q1/686 , C12Q1/6813 , C12Q1/6844 , C12Q1/6869 , G01N1/34 , G01N15/14
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
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公开(公告)号：US20180243741A1
公开(公告)日：2018-08-30
申请号：US15829665
申请日：2017-12-01
Applicant: Fluidigm Corporation
Inventor： Marc A. Unger , Lincoln McBride , Geoffrey Facer
IPC: B01L3/00 , C12Q1/686 , G01N27/453 , B01L7/00
CPC classification number: B01L3/502738 , B01L7/52 , B01L2200/147 , B01L2300/0636 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/0893 , B01L2300/123 , B01L2300/1805 , B01L2300/1827 , B01L2400/0481 , B01L2400/0655 , C12Q1/6848 , C12Q1/6851 , C12Q1/686 , C12Q2565/629 , G01N27/44791 , G01N27/453 , C12Q2561/113 , C12Q2563/159 , C12Q2531/113
Abstract: Devices and methods for performing the relative concentration of a target in a sample, the sample containing both target and non-target components, the method performed by partitioning the sample into a large number of reaction volumes such that the target is concentrated relative to the non-target, and performing a detection assay upon each reaction volume to detect the target.
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Patent Agency Ranking
公开(公告)号：US09952126B2
公开(公告)日：2018-04-24
申请号：US13781313
申请日：2013-02-28
Applicant: Fluidigm Corporation
Inventor： Brian Fowler , Jake Kimball , Myo Thu Maung , Andrew May , Michael C Norris , Dominique Toppani , Marc A. Unger , Jing Wang , Jason A. A. West
CPC classification number: G01N1/28 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0864 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12P19/34 , C12Q1/6813 , C12Q1/6844 , C12Q1/686 , C12Q1/6869 , G01N1/34 , G01N15/1484 , C12Q2565/629
Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.
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公开(公告)号：US20170175170A1
公开(公告)日：2017-06-22
申请号：US15382360
申请日：2016-12-16
Applicant: Fluidigm Corporation
Inventor： Peilin Chen
CPC classification number: B01J19/0046 , B01J2219/00585 , B01J2219/00722 , C12Q1/6848 , C12Q1/6853 , C12Q2525/161 , C12Q2525/301
Abstract: The present disclosure provides a “looping amplification” method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.
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公开(公告)号：US20170096713A1
公开(公告)日：2017-04-06
申请号：US15266787
申请日：2016-09-15
Applicant: California Institute of Technology , Fluidigm Corporation
Inventor： Stanley N. Lapidus , Stephen R. Quake
IPC: C12Q1/68
CPC classification number: C12Q1/6886 , C12Q1/6869 , C12Q2600/156
Abstract: The invention provides methods for determining the presence of a disease by comparing a sequence from a single target molecule with a predetermined sequence that is associated with a specific disease.
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公开(公告)号：US09593698B2
公开(公告)日：2017-03-14
申请号：US14072243
申请日：2013-11-05
Applicant: Fluidigm Corporation
Inventor： David Fernandes , Hou-Pu Chou , Marc A. Unger
CPC classification number: F15C3/00 , A61M2206/22 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L2200/0621 , B01L2200/14 , B01L2300/123 , B01L2400/0487 , B01L2400/0638 , B01L2400/0655 , F15C3/04 , F16K99/0001 , F16K99/0009 , F16K99/0015 , F16K99/0034 , F16K99/0059 , F16K2099/0074 , F16K2099/008 , F16K2099/0082 , F16K2099/0084 , G06F17/509 , G06F2217/16 , H01H2029/008 , Y10T137/206 , Y10T137/2202 , Y10T137/2218 , Y10T137/2224 , Y10T137/7879 , Y10T137/7891 , Y10T137/7892
Abstract: A microfabricated fluidic unidirectional valve includes a microfabricated elastomer material having a flow through channel. The microfabricated fluidic unidirectional valve also includes an elastomer flap attached to the elastomer material in the flow through channel. The elastomer flap forms a seal in the flow through channel to prevent fluid from flowing in a first direction through the flow through channel and to allow fluid flow in a second direction through the flow through channel.
Abstract translation: 微制造的流体单向阀包括具有流通通道的微加工弹性体材料。 微制造的流体单向阀还包括在流动通道中附接到弹性体材料的弹性体翼片。 弹性翼片在流通通道中形成密封，以防止流体沿第一方向流过通过通道并允许流体沿第二方向流过通过通道的流体。
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公开(公告)号：US20160362741A1
公开(公告)日：2016-12-15
申请号：US15013915
申请日：2016-02-02
Applicant: Fluidigm Corporation
Inventor： Stanley N. Lapidus
IPC: C12Q1/68
CPC classification number: C12Q1/68 , C12Q1/6869 , C12Q1/6874 , C12Q2565/518 , C12Q2563/107 , C12Q2533/101
Abstract: The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques.
Abstract translation: 本发明提供了使用固定的捕获探针和单分子测序技术来检测，列举或鉴定靶核酸分子的方法和装置。
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