公开(公告)号：EP1893975A2

公开(公告)日：2008-03-05

申请号：EP06784804.4

申请日：2006-06-12

Applicant: Stratagene California

Inventor： REID, Taylor, A. ,  TAYLOR, Roger, H. ,  ZAJAC, Kenneth, J.

IPC: G01N21/01

CPC classification number: G01J3/10 ,  G01J3/4406 ,  G01J2003/1213 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6486 ,  G01N2021/6484 ,  G01N2201/0446 ,  G01N2201/0612 ,  G01N2201/062

Abstract: A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source(40) that emits an excitation light (48) into an illumination tube (44); a plurality of collection optics (39) located around an aperture (47) in the illumination tube for collecting fluorescence; and a detector (53) for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light (48) from a light source (40) into an illumination tube (44); directing the excitation light to an excitation filter (62); illuminating a sample with the excitation light (48) to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics (39) located around the illumination tube (44).

公开(公告)号：EP1620572B1

公开(公告)日：2007-02-21

申请号：EP04751790.9

申请日：2004-05-10

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： KORDUNSKY, Igor ,  GOLDMAN, Jeffrey, A. ,  FINNEY, Michael, J.

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1822 ,  G01N21/276 ,  G01N21/6452 ,  G01N2201/0446

Abstract: A fluorescence detection apparatus for analyzing samples located in a plurality of wells in a thermal cycler and methods of use are provided. In one embodiment, the apparatus includes a support structure attachable to the thermal cycler and a detection module movably mountable on the support structure. The detection module includes one or more channels, each having an excitation light generator and an emission light detector both disposed within the detection module. When the support structure is attached to the thermal cycler and the detection module is mounted on the support structure, the detection module is movable so as to be positioned in optical communication with different ones of the plurality of wells. The detection module is removable from the support structure to allow easy replacement.

公开(公告)号：EP1620572A2

公开(公告)日：2006-02-01

申请号：EP04751790.9

申请日：2004-05-10

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： KORDUNSKY, Igor ,  GOLDMAN, Jeffrey, A. ,  FINNEY, Michael, J.

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/1822 ,  G01N21/276 ,  G01N21/6452 ,  G01N2201/0446

Abstract: A fluorescence detection apparatus for analyzing samples located in a plurality of wells in a thermal cycler and methods of use are provided. In one embodiment, the apparatus includes a support structure attachable to the thermal cycler and a detection module movably mountable on the support structure. The detection module includes one or more channels, each having an excitation light generator and an emission light detector both disposed within the detection module. When the support structure is attached to the thermal cycler and the detection module is mounted on the support structure, the detection module is movable so as to be positioned in optical communication with different ones of the plurality of wells. The detection module is removable from the support structure to allow easy replacement.

Abstract translation: 提供了用于分析位于热循环仪中的多个孔中的样品的荧光检测设备以及使用方法。 在一个实施例中，该设备包括可附接到热循环仪的支撑结构和可移动地安装在支撑结构上的检测模块。 检测模块包括一个或多个通道，每个通道均具有设置在检测模块内的激发光发生器和发射光检测器。 当支撑结构被连接到热循环仪并且检测模块被安装在支撑结构上时，检测模块是可移动的，从而被定位成与多个井中不同的井进行光通信。 检测模块可从支撑结构上拆下，以便于更换。

公开(公告)号：EP0545673B1

公开(公告)日：1995-11-15

申请号：EP92310975.5

申请日：1992-12-01

Applicant: SEIKAGAKU KOGYO KABUSHIKI KAISHA

Inventor： Hiroshi, Tamura ,  Shigenori, Tanaka

IPC: B01L7/02 ,  B01L3/00 ,  G01N21/03

CPC classification number: B01L3/50851 ,  B01L3/50853 ,  B01L7/02 ,  G01N21/031 ,  G01N21/0332 ,  G01N21/253 ,  G01N2021/0325 ,  G01N2201/0446 ,  Y10S436/809

公开(公告)号：EP0660924A1

公开(公告)日：1995-07-05

申请号：EP94921655.0

申请日：1994-07-18

Applicant: BALZERS AKTIENGESELLSCHAFT ,  Artificial Sensing Instruments ASI AG

Inventor： RUDIGIER, Helmut ,  PFEFFERKORN, Roland ,  TIEFENTHALER, Kurt ,  BRIGUET, Véronique ,  ZETTING, Alois ,  MARBACH, Peter ,  LEGAY, François ,  END, Peter

IPC: G01N33 ,  B01L3 ,  G01N21

CPC classification number: G01N21/253 ,  B01L3/5085 ,  B01L2300/0654 ,  G01N21/4788 ,  G01N21/7743 ,  G01N2201/0446

Abstract: A detection cell (2) which is used as a component of an optical biosensor comprises a transparent base plate (8) and a sample plate (4) on the base plate. The sample plate has a matrix of wells (6) extending through it to each receive a sample. The base plate includes a waveguiding film and a diffraction grating means to incouple an incident light field into the waveguiding film beneath a well to generate a diffracted light field to enable the detection of a change in the effective refractive index of the waveguiding film.

公开(公告)号：EP0588931A1

公开(公告)日：1994-03-30

申请号：EP92913497.0

申请日：1992-06-11

Applicant: ABBOTT LABORATORIES

Inventor： CHOW, Herbert, S. ,  WILSON, Thomas, J. ,  WROBLEWSKI, Mieczyslaw ,  KOTLARIK, John, J. ,  ALSBERG, Keith, D. ,  McCOY, Jimmy, D. ,  LADD, Jimmy, L. ,  CORBETT, Kevin, M.

IPC: G01N21 ,  B01L3 ,  G01N15 ,  G01N33 ,  G01N35 ,  G02B7 ,  G06T1

CPC classification number: G01N35/109 ,  B01L3/0262 ,  B01L3/5085 ,  G01N15/1468 ,  G01N21/82 ,  G01N35/0095 ,  G01N35/0098 ,  G01N35/028 ,  G01N35/1004 ,  G01N2035/042 ,  G01N2035/0425 ,  G01N2035/0491 ,  G01N2035/1018 ,  G01N2035/1025 ,  G01N2035/1034 ,  G01N2201/0446 ,  G01N2201/0461 ,  G01N2201/0492 ,  G02B7/28 ,  Y10T137/7306 ,  Y10T436/115831

Abstract: Appareil assurant le transfert d'une cartouche de réaction (10) vers différentes zones de traitement (36, 38, 42). Des dispositifs automatiques (34, 40) assurent le transfert de la cartouche de réaction vers différentes zones de l'appareil (30, 32, 36, 38, 42, 46). Un dispositif de formation d'image (42) détecte des images indiquant si telle réaction s'est produite dans tel puits de réaction (16) de la cartouche de réaction (10). L'appareil comporte également un dispositif (36) d'aspiration et de distribution des liquides assorti d'un sytème de détection du niveau de liquide (70, 72, 74, 76, 78, 80, 82, 84). L'appareil comporte en outre un micro-processeur et une logique (44) de contrôle de fonctionnement et d'analyse des informations reçues du dispositif de formation d'image (42) afin de produire une indication visuelle des résultats des analyses.

公开(公告)号：EP0565693A1

公开(公告)日：1993-10-20

申请号：EP92922900.0

申请日：1992-10-27

Applicant: BAXTER DIAGNOSTICS INC.

Inventor： GRINER, Christopher, D.

IPC: G01N21 ,  G01N35

CPC classification number: G01N21/253 ,  G01N21/274 ,  G01N35/028 ,  G01N2201/0407 ,  G01N2201/0446 ,  G01N2201/08 ,  G01N2201/1283

Abstract: Un système (10) utilise un photomètre qui établit un canal optique entre un émetteur de lumière (102) et un détecteur de lumière (106). Le photomètre analyse un spécimen lorsqu'il se trouve dans l'avéole (48) d'un plateau (12). Une plate-forme mobile (74) transporte le plateau (12) vers le canal optique en vue d'une analyse et retire le plateau (12) du canal optique après l'analyse. Le système calibre la plate-forme mobile (74) afin d'assurer un alignement correct entre le spécimen et l'appareil photométrique utilisé pour analyser le spécimen. Le système calibre également le canal optique sans utiliser une référence externe.

公开(公告)号：EP0542913A4

公开(公告)日：1993-06-30

申请号：EP91916534

申请日：1991-08-07

Applicant: MACRI, JAMES N. ,  COOK, EDWARD J.

Inventor： MACRI, JAMES N. ,  COOK, EDWARD J.

IPC: G01N21/27 ,  G01J1/02

CPC classification number: G01N21/274 ,  G01N2201/0446 ,  G01N2201/13

公开(公告)号：EP0148736B1

公开(公告)日：1988-05-04

申请号：EP84850299.3

申请日：1984-10-09

Applicant: WALLAC OY

Inventor： Soini, Erkki

IPC: G01N21/76

CPC classification number: G01N21/76 ,  G01N2201/0446

公开(公告)号：WO2016123157A1

公开(公告)日：2016-08-04

申请号：PCT/US2016/015002

申请日：2016-01-26

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： KIM, Jinho ,  YANG, Changhuei

IPC: G01N21/62 ,  C12M1/34

CPC classification number: G02B21/365 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6458 ,  G01N2021/6478 ,  G01N2201/0446 ,  G02B21/0076 ,  G02B21/16 ,  H04N5/2258

Abstract: An imaging device for bright field Fourier ptychographic imaging and fluorescence imaging comprises a first fluorescence illumination source configured to provide excitation light of a first range of wavelengths to a transparent well; an optical system having a pair of objectives positioned oppositely; and an image sensor configured to receive light propagated through the optical system from the transparent well. The image sensor is configured to acquire a sequence of uniquely illuminated intensity measurements of light passing through a sample in the well based on sequential illumination at different illumination angles from a variable illumination source, and further configured to acquire a first fluorescence image of the sample based on light emitted by the sample in response to excitation light of the first range of wavelengths.

Abstract translation: 一种用于明场傅立叶印刷成像和荧光成像的成像设备，包括：第一荧光照射源，被配置为将第一波长范围的激发光提供给透明孔; 具有相对放置的一对物镜的光学系统; 以及图像传感器，被配置为接收从透明井传播通过光学系统传播的光。 图像传感器被配置为基于来自可变照明源的不同照射角度的顺序照射来获取穿过阱中样本的光的唯一照射强度测量的序列，并且还被配置为获取基于样本的第一荧光图像 对由样品响应于第一波长范围的激发光而发射的光进行测量。