公开(公告)号：US20210364494A1

公开(公告)日：2021-11-25

申请号：US16635911

申请日：2018-08-01

Applicant: ACEA BIOSCIENCES, INC.

Inventor： Nan Li ,  Yama Abassi ,  Xiao Xu ,  Xiaobo Wang

IPC: G01N33/483 ,  G01N1/14 ,  G01N27/02

Abstract: A method of assessing cytolysis of cancer cells, the method including: providing a cell substrate impedance monitoring cartridge having a plurality of chambers, each chamber having an electrode array configured for measuring cell-substrate impedance, wherein different chambers are preloaded with different target cells embodied as cancer cells of different lineage, origin or stage; adding effector cells to the plurality chambers for interaction with the target cells, wherein the effector cells are immune cells; monitoring cell-substrate impedance of the plurality of chambers before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedances; and determining effectiveness of effector cell killing of the different target cells by comparing the impedances or impedance-based parameters over time.

公开(公告)号：US20200278339A1

公开(公告)日：2020-09-03

申请号：US16752298

申请日：2020-01-24

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Wei Ouyang ,  Nan Li ,  Biao Xi ,  Yama A. Abassi ,  Xiao Xu

IPC: G01N33/487

Abstract: Extracellular recording devices with reference electrodes positioned in a cell free zone and methods of conducting extracellular recording. Parallel cell-substrate impedance monitoring and extracellular recording of excitable cells such as excitable cells, cardiomyocytes, and cardiomyocyte precursor cells using devices that switch between extracellular recording and impedance monitoring modes.

公开(公告)号：US10648913B2

公开(公告)日：2020-05-12

申请号：US15415439

申请日：2017-01-25

Applicant: ACEA BIOSCIENCES, INC

Inventor： Nan Li ,  Jian Wu ,  Ye Chen ,  Tianxing Wang ,  Xiaobo Wang

IPC: G01N21/64 ,  G01N15/14 ,  G01N15/10

Abstract: A system for detecting signal components of light induced by multiple excitation sources, which includes a flow channel having two spatially separated optical interrogation zones; a light illumination subsystem that directs each of two light beams of different wavelengths to a different zone of the optical interrogation zones; a detector subsystem that converts detected light into a total electrical signal having both modulated and unmodulated electrical signals; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

公开(公告)号：US10018581B2

公开(公告)日：2018-07-10

申请号：US13802174

申请日：2013-03-13

Applicant: ACEA BIOSCIENCES, INC

Inventor： Xiaobo Wang ,  Xiao Xu

IPC: B01L3/00 ,  G01N27/02 ,  C12M1/32 ,  C12M1/34 ,  G01N27/327

CPC classification number: G01N27/02 ,  C12M23/12 ,  C12M41/36 ,  G01N27/3276 ,  Y10T29/49117

Abstract: A method for assaying target molecules in a sample liquid, the method comprising: providing an impedance monitoring device operably connected to an impedance analyzer; adding a sample liquid suspected of having target molecules to the well thereby permitting binding of target molecules to the capture molecules; monitoring impedance of the well; and determining the presence, amount or concentration of target molecules in the liquid sample from the monitored impedance. The device includes a nonconducting substrate having a well, at least two electrodes fabricated on a bottom of the well and on a same plane, wherein the surfaces of the at least two electrodes are modified with capture molecules configured to bind target molecules in a liquid sample, and at least two connection pads electrically connected to the at least two electrodes.

公开(公告)号：US20170370907A1

公开(公告)日：2017-12-28

申请号：US15613052

申请日：2017-06-02

Applicant: ACEA Biosciences, Inc.

Inventor： Yama A. Abassi ,  Naichen Yu ,  Josephine Atienza ,  Xiao Xu ,  Xiaobo Wang

IPC: G01N33/50 ,  G01N33/543 ,  C12Q1/00 ,  G01N27/02

CPC classification number: G01N33/5041 ,  C12Q1/002 ,  G01N27/021 ,  G01N33/5014 ,  G01N33/5438 ,  G01N2333/4716 ,  G01N2333/705 ,  G01N2333/91205 ,  G01N2500/04 ,  G01N2500/10

Abstract: A method of identifying a potential therapeutic compound that affects a Receptor Tyrosine Kinase (RTK) pathway in cancer cells, which includes: providing a device capable of measuring cell-substrate impedance; culturing cancer cells in serum-free media in at least two wells of the device; adding to a first well a proposed therapeutic compound that affects a RTK pathway and a RTK stimulating factor for the RTK pathway to form a test well, and adding to another well the RTK stimulating factor to form a control well; continuously monitoring cell-substrate impedance of the at least two wells and optionally determining cell indices from the monitored cell-substrate impedance; and determining a difference in impedance or optionally cell index between the test well and control well; and if significantly different, concluding the proposed therapeutic compound is therapeutically active in the RTK pathway within the cancer cells.

公开(公告)号：US20170138856A1

公开(公告)日：2017-05-18

申请号：US15416976

申请日：2017-01-26

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Ye Chen ,  Hyunsun Chung ,  Zengqiang Li ,  Xiaobo Wang

IPC: G01N21/64 ,  G01N15/14

CPC classification number: G01N21/645 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/6486 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/06113

Abstract: An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.

公开(公告)号：US20170131206A1

公开(公告)日：2017-05-11

申请号：US15415439

申请日：2017-01-25

Applicant: ACEA BIOSCIENCES, INC

Inventor： Nan Li ,  Jian Wu ,  Ye Chen ,  Tianxing Wang ,  Xiaobo Wang

IPC: G01N21/64 ,  G01N15/14

CPC classification number: G01N21/645 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/64 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1438 ,  G01N2015/149 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/06113 ,  G01N2201/0621

Abstract: A system for detecting signal components of light induced by multiple excitation sources, which includes a flow channel having two spatially separated optical interrogation zones; a light illumination subsystem that directs each of two light beams of different wavelengths to a different zone of the optical interrogation zones; a detector subsystem that converts detected light into a total electrical signal having both modulated and unmodulated electrical signals; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

公开(公告)号：US09568423B2

公开(公告)日：2017-02-14

申请号：US13657845

申请日：2012-10-22

Applicant: ACEA BIOSCIENCES, INC

Inventor： Nan Li ,  Jian Wu ,  Ye Chen ,  Tianxing Wang ,  Xiaobo Wang

IPC: G01N21/64 ,  G01F1/00 ,  G01N15/14 ,  G01N15/10

CPC classification number: G01N21/645 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/64 ,  G01N21/6428 ,  G01N2015/1006 ,  G01N2015/1438 ,  G01N2015/149 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/06113 ,  G01N2201/0621

Abstract: A system for detecting signal components of light induced by multiple excitation sources including: a flow channel including at least two spatially separated optical interrogation zones; a non-modulating excitation source that directs a light beam of a first wavelength at a near constant intensity onto a first of the optical interrogation zones; a modulating excitation source that directs a light beam of a second wavelength with an intensity modulated over time at a modulating frequency onto a second of the optical interrogation zones; a detector subsystem comprising a set of detectors configured to detect light emitted from particles flowing through the at least two optical interrogation zones and to convert the detected light into a total electrical signal; and a processor that determines signal components from the light detected from each of the optical interrogation zones.

Abstract translation: 一种用于检测由多个激发源引起的光的信号分量的系统，包括：流路，包括至少两个空间分离的光询问区; 非调制激励源，其将第一波长的光束以接近恒定的强度引导到第一光学询问区上; 调制激励源，其将以调制频率随时间调制的第二波长的光束引导到第二光学询问区上; 检测器子系统，包括一组检测器，其被配置为检测从流过所述至少两个光询问区的颗粒发射的光并将检测到的光转换成总电信号; 以及处理器，其根据从每个光询问区检测的光来确定信号分量。

公开(公告)号：US20140057283A1

公开(公告)日：2014-02-27

申请号：US13802174

申请日：2013-03-13

Applicant: ACEA BIOSCIENCES, INC

Inventor： Xiaobo Wang ,  Xiao Xu

IPC: G01N27/02

CPC classification number: G01N27/02 ,  C12M23/12 ,  C12M41/36 ,  G01N27/3276 ,  Y10T29/49117

Abstract: A method for assaying target molecules in a sample liquid, the method comprising: providing an impedance monitoring device operably connected to an impedance analyzer; adding a sample liquid suspected of having target molecules to the well thereby permitting binding of target molecules to the capture molecules; monitoring impedance of the well; and determining the presence, amount or concentration of target molecules in the liquid sample from the monitored impedance. The device includes a nonconducting substrate having a well, at least two electrodes fabricated on a bottom of the well and on a same plane, wherein the surfaces of the at least two electrodes are modified with capture molecules configured to bind target molecules in a liquid sample, and at least two connection pads electrically connected to the at least two electrodes.

Abstract translation: 一种用于测定样品液体中的靶分子的方法，所述方法包括：提供可操作地连接到阻抗分析仪的阻抗监测装置; 将怀疑具有靶分子的样品液体加入到孔中，从而允许靶分子与捕获分子的结合; 监测井的阻抗; 以及从所监测的阻抗确定液体样品中靶分子的存在，量或浓度。 该器件包括具有阱的非导电衬底，至少两个电极，其制造在阱的底部并且在同一平面上，其中至少两个电极的表面被配置成结合在液体样品中的靶分子结合的俘获分子 以及至少两个电连接到所述至少两个电极的连接焊盘。

公开(公告)号：US20140038981A1

公开(公告)日：2014-02-06

申请号：US13917514

申请日：2013-06-13

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao XU ,  Xiaobo WANG ,  Long MAO ,  Li ZHAO ,  Biao XI

IPC: C07D487/04

CPC classification number: A61K31/519 ,  A61K45/06 ,  C07D487/04 ,  A61K2300/00

Abstract: The present invention relates to certain pyrrolopyrimidine derivatives, pharmaceutical compositions containing them, and methods of using them, including methods for the treatment of tumors and related diseases related to the dysregulation of kinase (such as EGFR (including HER), Alk, PDGFR, but not limited to) pathways.