Abstract:
PROBLEM TO BE SOLVED: To provide a method for producing a protein A immobilization carrier preventing protein A from leaking in a large amount in purifying an IgG antibody, the protein A being contained in the protein A immobilization carrier that adsorbs antibodies in antibody affinity chromatography.SOLUTION: A carrier 1 and an epoxy group-containing compound containing a terminal epoxy group 2 as a linker precursor 10 are reacted so that the amount of the epoxy group-containing compound per specific surface area of the carrier is 1.5 μmol/mor more and then reacted with a diamine to form a linker 20 and further added with the protein A to produce the protein A immobilization carrier. The protein A immobilization carrier reduces the amount of the protein A that leaks in purifying an IgG antibody, and improves resistance to alkali CIP (cleaning in place).
Abstract:
PROBLEM TO BE SOLVED: To provide a thin film chromatographic separating material further improved in separating performance the component of which is entirely formed of silica. SOLUTION: In this thin chromatographic separating material having a thin film containing silica gel fine particles, which is formed on a support base as a separating layer, leaf silica secondary particle and colloidal silica particle are used as binder.
Abstract:
PROBLEM TO BE SOLVED: To provide a method of manufacturing porous inorganic powder having excellent re-dispersibility without causing hard caking in a water-based medium when the powder is blended in the water-based medium. SOLUTION: The method of manufacturing the porous inorganic powder having excellent re-dispersibility includes: a first step for adding the solution of an organic solvent containing a silicone-based compound into the porous inorganic powder having fine pores in a quantity equivalent to fill 20-70 vol% of pore volume and introducing the silicone-based compound into the fine pores while mixing and stirring substantially in a dry state; and a second step for heating the treated inorganic powder to dry the inorganic powder and to remove the organic solvent and supporting the silicone-based compound in the fine pores. Silica gel, zeolite, carbon black or the like is preferably used as the porous inorganic powder. Methyl hydrogen silicone oil, dimethyl silicone oil, epoxy-modified silicone oil, amino-modified silicone oil and the like are preferably used as the silicone-based compound. COPYRIGHT: (C)2009,JPO&INPIT