公开(公告)号：EP3302802B1

公开(公告)日：2019-09-04

申请号：EP16736297.9

申请日：2016-06-06

Applicant: Douglas Scientific, LLC

Inventor： HJELSETH, Robert ,  HODGSON, Cory ,  BRISTOW, Andrew ,  BAUMGARTNER, Mark ,  SELLNOW, David ,  KEPPEL, Cassie ,  STECKELBERG, John ,  NEWMAN, Paul

IPC: B01L3/00 ,  G01N1/28 ,  C12M3/00 ,  C12M1/33

公开(公告)号：EP3302802A2

公开(公告)日：2018-04-11

申请号：EP16736297.9

申请日：2016-06-06

Applicant: Douglas Scientific, LLC

Inventor： HJELSETH, Robert ,  HODGSON, Cory ,  BRISTOW, Andrew ,  BAUMGARTNER, Mark ,  SELLNOW, David ,  KEPPEL, Cassie ,  STECKELBERG, John ,  NEWMAN, Paul

IPC: B01L3/00 ,  G01N1/28 ,  C12M3/00 ,  C12M1/33

CPC classification number: B01L3/5635 ,  B01L3/50 ,  B01L3/502 ,  B01L3/508 ,  B01L3/561 ,  B01L2200/026 ,  B01L2200/0631 ,  B01L2300/021 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/0832 ,  B01L2400/0457 ,  C12M45/06 ,  C12N15/1017

Abstract: Embodiments of the invention provide a filter kit including filters for processing a biological sample. Some embodiments include a filter cap in a tube kit with a first tube containing a buffer solution and a second tube containing a lyophilized master mix. Some embodiments include a method of processing a sample using the kit including mixing a biological sample in a first tube with the buffer solution, positioning the filter cap in the first tube, positioning a second tube on the filter cap, flipping the first tube, the filter cap, and the second cap to filter the biological sample and buffer solution mixture with the filter cap as it flows from the first tube to the second tube. Some embodiments include structure enabling transfer of materials through inline flow between the tubes. Some further embodiments include integrated structure for sample pulverization with integrated buffer and lyophilized master mix.

公开(公告)号：EP2972408A1

公开(公告)日：2016-01-20

申请号：EP14762494.4

申请日：2014-03-14

Applicant: Douglas Scientific, LLC

Inventor： SCHOENECK, Richard J. ,  WAGNER, Paul H.

IPC: G01N35/10 ,  G01N1/14 ,  B01L3/02

CPC classification number: B01L3/0217 ,  B01L3/0234 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2300/0829 ,  B01L2300/0861 ,  B08B3/12 ,  B08B9/023 ,  B08B9/0323 ,  G01N35/1004 ,  G01N35/1065

Abstract: A pipettor includes a wash chamber between an upper plate, a lower plate, an upper seal, and a lower seal. The upper and lower seal retain each other and are located between the upper and lower plates. The pipettor includes a gasket located below the lower plate, a pipette tip retained by the gasket, and a piston with a tapered tip that passes through the plates, seals, and gasket into the pipette tip. The upper and lower seal isolate the piston from the wash chamber. The pipettor includes a channel defined by the piston, extending into the pipette tip, and an actuator that advances and retracts the piston. When the actuator retracts the piston such that the piston loses sealing contact with the lower seal and the wash chamber is supplied with wash fluid and pressurized, wash fluid passes through the groove in the upper seal and into the channel.

Abstract translation: 移液器包括上板，下板，上部密封件和下部密封件之间的洗涤室。 上部和下部密封件彼此保持并位于上部和下部板之间。 移液器包括位于下板下方的垫圈，由垫圈保持的移液管尖端和带有锥形尖端的活塞，其穿过板，密封件和垫圈进入移液管尖端。 上部和下部密封将活塞与洗涤室隔离。 移液器包括由活塞限定的通道，延伸到移液管尖端中，以及致动器，其使活塞前进和缩回。 当致动器缩回活塞使得活塞失去与下密封件的密封接触并且洗涤室被供应有洗涤流体并被加压时，洗涤流体穿过上密封件中的凹槽并进入通道。

公开(公告)号：EP2809768A1

公开(公告)日：2014-12-10

申请号：EP13743751.3

申请日：2013-02-04

Applicant: Douglas Scientific

Inventor： WAGNER, Paul, Howard ,  HAQ, Adnanul ,  SCHOENECK, Richard, Jerome

IPC: C12M1/34

CPC classification number: G01N35/028 ,  G01N21/6452 ,  G01N35/00009 ,  G01N2035/00356 ,  G01N2035/00455 ,  G01N2035/0403 ,  G01N2035/0418 ,  G01N2035/0422 ,  G01N2035/0465 ,  G01N2201/0446

Abstract: A high throughput biological sample processing system includes a sample carrier with a plurality of wells that progresses through the high throughput biological sample processing system. The system further includes a sample dispensing module, a reagent dispensing module, an accumulation/incubation module, and a detection module. The detection module employs an optical measuring device to encapsulate a biological sample in one of the plurality of wells of the sample carrier and detect energy from the chemistry of the biological sample to determine the amount of an analyte in the biological sample.

公开(公告)号：WO2014144221A2

公开(公告)日：2014-09-18

申请号：PCT/US2014/028533

申请日：2014-03-14

Applicant: DOUGLAS SCIENTIFIC

Inventor： MISCHE, Hans, Alois ,  COOK, Darren, Lynn

IPC: G01N1/24

CPC classification number: G01N35/021 ,  B01L3/505 ,  B01L3/50851 ,  B01L2200/12 ,  B32B38/04 ,  B32B38/06 ,  B32B2535/00 ,  G01N35/00009 ,  G01N35/04 ,  G01N35/1002 ,  G01N2035/023 ,  G01N2035/0437

Abstract: A system for processing and analyzing a sample includes a belt with wells that proceeds through the system, a dispensing station that dispenses the sample and reagents into the wells of the belt, and a detection station that detects an analyte in the wells of the belt. The system further includes a wash and decontamination station for decontaminating the wells of the belt.

Abstract translation: 用于处理和分析样品的系统包括具有穿过系统的孔的带，将样品和试剂分配到带的孔中的分配站以及检测带的孔中的分析物的检测站。 该系统还包括用于净化带的孔的洗涤和去污站。

公开(公告)号：WO2014144132A1

公开(公告)日：2014-09-18

申请号：PCT/US2014/028415

申请日：2014-03-14

Applicant: DOUGLAS SCIENTIFIC

Inventor： MISCHE, Hans Alois ,  HJELSETH, Robert, Martin

IPC: B32B37/02 ,  B32B37/04 ,  B32B37/10

CPC classification number: G01N35/021 ,  B01L3/505 ,  B01L3/50851 ,  B01L2200/12 ,  B32B38/04 ,  B32B38/06 ,  B32B2535/00 ,  G01N35/00009 ,  G01N35/04 ,  G01N35/1002 ,  G01N2035/023 ,  G01N2035/0437

Abstract: A method of forming a tape with a matrix of wells includes patterning a first surface of a substrate. The substrate includes the first surface and a planar second surface opposite the first surface. Patterning the first surface of the substrate forms the matrix of wells of the tape. The second surface of the substrate remains planar upon patterning the first surface of the substrate.

Abstract translation: 形成具有阱矩阵的带的方法包括对衬底的第一表面进行图案化。 衬底包括第一表面和与第一表面相对的平面第二表面。 对衬底的第一表面进行图案化形成磁带的孔的矩阵。 在图案化衬底的第一表面之后，衬底的第二表面保持平坦。

公开(公告)号：WO2016154555A1

公开(公告)日：2016-09-29

申请号：PCT/US2016/024260

申请日：2016-03-25

Applicant: DOUGLAS SCIENTIFIC, LLC

Inventor： MISCHE, Hans ,  PEXSA, Grant ,  BAUMGARTNER, Mark ,  HODGSON, Cory ,  BRISTOW, Andrew ,  STECKELBERG, John ,  PATTERSON, Jared

IPC: G01N33/48 ,  G01N21/63 ,  C12M1/34

CPC classification number: G01N21/6428 ,  B01L3/527 ,  B01L7/52 ,  B01L2300/0609 ,  B01L2300/0893 ,  G01N21/253 ,  G01N21/645 ,  G01N33/50 ,  G01N2021/6439 ,  G01N2021/6482 ,  G01N2201/024 ,  G01N2201/062 ,  G01N2201/0691

Abstract: A modular testing device includes a base unit and an expansion unit that communicates with the base unit. The expansion unit includes a housing, a receptacle in which a sample holder containing a biological sample and reagent mixture can be placed, and an optical assembly positioned in the housing. The optical assembly is configured to amplify and detect a signal from the biological sample and reagent mixture. Data that is collected in the optical assembly is communicated to the base unit.

Abstract translation: 模块化测试装置包括与基本单元通信的基本单元和扩展单元。 膨胀单元包括壳体，其中可以放置容纳生物样品和试剂混合物的样品保持器的容器和位于壳体中的光学组件。 光学组件被配置为放大并检测来自生物样品和试剂混合物的信号。 收集在光学组件中的数据被传送到基本单元。

公开(公告)号：WO2014144201A1

公开(公告)日：2014-09-18

申请号：PCT/US2014/028505

申请日：2014-03-14

Applicant: DOUGLAS SCIENTIFIC

Inventor： SCHOENECK, Richard J. ,  WAGNER, Paul H.

IPC: G01N35/10 ,  G01N1/14 ,  B01L3/02

CPC classification number: B01L3/0217 ,  B01L3/0234 ,  B01L2200/0689 ,  B01L2200/141 ,  B01L2300/0829 ,  B01L2300/0861 ,  B08B3/12 ,  B08B9/023 ,  B08B9/0323 ,  G01N35/1004 ,  G01N35/1065

Abstract: A pipettor includes a wash chamber between an upper plate, a lower plate, an upper seal, and a lower seal. The upper and lower seal retain each other and are located between the upper and lower plates. The pipettor includes a gasket located below the lower plate, a pipette tip retained by the gasket, and a piston with a tapered tip that passes through the plates, seals, and gasket into the pipette tip. The upper and lower seal isolate the piston from the wash chamber. The pipettor includes a channel defined by the piston, extending into the pipette tip, and an actuator that advances and retracts the piston. When the actuator retracts the piston such that the piston loses sealing contact with the lower seal and the wash chamber is supplied with wash fluid and pressurized, wash fluid passes through the groove in the upper seal and into the channel.

Abstract translation: 移液器包括在上板，下板，上密封和下密封之间的洗涤室。 上下密封彼此保持并位于上板和下板之间。 移液器包括位于下板下方的垫圈，由垫圈保持的移液管末端，以及具有锥形尖端的活塞，其通过板，密封件和垫圈进入移液管尖端。 上下密封将活塞与洗涤室隔离开。 移液器包括由活塞限定的通道，延伸到移液管末端，以及促进和缩回活塞的致动器。 当致动器缩回活塞，使得活塞与下部密封件密封接触，并且洗涤室被供应洗涤流体并加压，洗涤流体通过上部密封件中的凹槽并进入通道。

公开(公告)号：WO2016191582A1

公开(公告)日：2016-12-01

申请号：PCT/US2016/034390

申请日：2016-05-26

Applicant: DOUGLAS SCIENTIFIC, LLC

Inventor： HENDRICKX, Jacob, P. ,  SMITH, Chad, S. ,  MISCHE, Hans, A. ,  BOTZET, Michael

IPC: A01C1/06 ,  B02C18/06 ,  B02C23/18 ,  B03B11/00 ,  B65G65/30

CPC classification number: G01N1/286 ,  G01N1/38 ,  G01N2001/2866 ,  G01N2001/4088

Abstract: A seed grinder includes a housing that is configured to contain seeds and a buffer solution, a grinding mechanism that is positionable in the housing to grind the seeds in the housing, and a dispensing mechanism attached to the housing and configured to dispense the ground seeds and the buffer solution from the housing into a sample holder.

Abstract translation: 种子研磨机包括构造成容纳种子和缓冲溶液的壳体，可定位在壳体中以研磨壳体中的种子的研磨机构以及附接到壳体并被配置为分配研磨种子的分配机构， 缓冲溶液从壳体进入样品架。

公开(公告)号：WO2016018910A1

公开(公告)日：2016-02-04

申请号：PCT/US2015/042471

申请日：2015-07-28

Applicant: DOUGLAS SCIENTIFIC, LLC

Inventor： COOK, Darren, Lynn ,  JOHNSON, Eric, Guy ,  WESTAD, Nathan, Luther ,  HAUG, Andrew, Richard ,  KONYNENBELT, James, Henry ,  MAASJO, Grant, Edward ,  PATTERSON, Jared, Whittier ,  URKE, Brent, Conrad ,  ZITZMANN, Ryan, John ,  SMITH, Chad, Steven

IPC: G01N35/02

CPC classification number: G01N35/00009 ,  B01L3/502 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0605 ,  B01L2200/0689 ,  B01L2200/14 ,  B01L2300/021 ,  B01L2300/0627 ,  B01L2300/0812 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/1827 ,  C12Q1/686 ,  G01N35/00029 ,  G01N35/0099 ,  G01N35/04 ,  G01N35/10 ,  G01N35/1004 ,  G01N35/1065 ,  G01N2035/00148 ,  G01N2035/00346 ,  G01N2035/00366 ,  G01N2035/00376 ,  G01N2035/00445 ,  G01N2035/0405 ,  G01N2035/0422 ,  G01N2035/0425 ,  G01N2035/0465

Abstract: An instrument for processing a biological sample includes a chassis. Connected to the chassis is a tape path along which a tape with a matrix of wells can be automatically advanced through the instrument, a dispensing assembly for dispensing the biological sample and a reagent into the matrix of wells of the tape to form a biological sample and reagent mixture, a sealing assembly for sealing the biological sample and reagent mixture in the tape, and an amplification and detection assembly for detecting a signal from the biological sample and reagent mixture in the matrix of wells in the tape.

Abstract translation: 用于处理生物样品的仪器包括底盘。 连接到底盘的是磁带路径，具有孔矩阵的带可以自动地通过仪器前进，用于将生物样品和试剂分配到带的孔的基质中的分配组件以形成生物样品， 试剂混合物，用于密封带中的生物样品和试剂混合物的密封组件，以及用于检测来自带中的孔中的生物样品和试剂混合物的信号的扩增和检测组件。