公开(公告)号：EP0851228A1

公开(公告)日：1998-07-01

申请号：EP97924357.3

申请日：1997-06-09

Applicant: Laboratory of Molecular Biophotonics

Inventor： HOSOI, Shigeru, Lab. of Molecular Biophotonics ,  KOJIMA, Makiko ,  KADOUCHI, Sachiko, Lab. of Molecular Biophotonics

IPC: G01N33/533 ,  C12Q1/68

CPC classification number: G01N33/582 ,  C12Q1/6834 ,  G01N33/533 ,  Y10S435/968 ,  Y10S436/828 ,  C12Q2563/107 ,  C12Q2545/114 ,  C12Q2565/601

Abstract: This invention provides fluoroimmunoassay which comprises labeling analyte molecules with a fluorescent material having a nucleic acid portion stained with a sufficient number of fluorochrome molecules so as to be measurable as fluorescent spots, and a reactive group binding to the analyte molecule specifically, immobilizing the labeled analyte on a solid phase, and counting the number of fluorescent spots. The nucleic acid portion of the fluorescent labeling material is a double-stranded or single-stranded nucleic acid, and the staining with the fluorochrome molecules is performed with intercalating type, minor groove binding type, or covalently binding to the nucleic acid type.

Abstract translation: 本发明提供荧光免疫测定法，其包括用荧光材料标记分析物分子，所述荧光材料具有用足够数量的荧光染料分子染色的核酸部分以便可测量为荧光斑点，以及特异性结合分析物分子的反应性基团，将标记的分析物 在固相上，并计数荧光斑数。 荧光标记材料的核酸部分是双链或单链核酸，并且用插入型，小沟结合型或共价结合核酸类型进行用荧光染料分子的染色。

公开(公告)号：EP0756009A2

公开(公告)日：1997-01-29

申请号：EP96111302.4

申请日：1996-07-12

Applicant: Laboratory of Molecular Biophotonics

Inventor： Sato, Yoshihiro

IPC: C12Q1/68

CPC classification number: C12Q1/682 ,  C12Q1/6862

Abstract: The present invention relates to a method for the amplification of a base sequence A1A2 consisting of two successive base sequences, A1 and A2, in a single strand polynucleotide as an object to be amplified characterized in that said method comprising at least (1) hybridizing a probe B consisting of a polynucleotide comprising the base sequence B1 complementary to said base sequence A1, a polynucleotide comprising the base sequence B2 complementary to said base sequence A2 and a linkage portion linking the two polynucleotide via itself, with the base sequence A1A2 of said polynucleotide, as the object to be amplified, to form a hybrid, (2) ligating the 5'-end of the base sequence B1 in said probe B to the 3'-end of the base sequence B2 in the same with the aid of ligase, (3) heat denaturating the double strand formed said hybridization, (4) hybridizing said heat denaturated polynucleotide complex with another probe B or a probe A consisting of a polynucleotide comprising said base sequence A1, a polynucleotide comprising said base sequence A2 and a linkage portion linking the two polynucleotide via itself, and (5) ligating 5'-end of the base sequence B1 in said probe B to the 3'-end of the base sequence B2 in the same, or 3'-end of the base sequence A1 in said probe A to the 5'-end of the base sequence A2 in the same with the aid of ligase.