-
公开(公告)号：US09745617B2
公开(公告)日：2017-08-29
申请号：US15019355
申请日：2016-02-09
Applicant: Raindance Technologies, Inc.
Inventor： Jonathan William Larson , Qun Zhong , Darren R. Link
IPC: C12Q1/68
CPC classification number: C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6883 , C12Q1/6886 , C12Q2600/172 , C12Q2563/159 , C12Q2565/629 , C12Q2537/143 , C12Q2531/113 , C12Q2535/131
Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
-
公开(公告)号：US20170028365A1
公开(公告)日：2017-02-02
申请号：US15290378
申请日：2016-10-11
Applicant: Raindance Technologies, Inc.
Inventor： Darren Link , Brian Hutchison , Michael Samuels , Michael Weiner
CPC classification number: B01F3/0807 , B01F5/0646 , B01F5/0653 , B01F13/0062 , B01F2215/0037 , B01J19/0046 , B01J2219/00286 , B01J2219/00351 , B01J2219/00418 , B01J2219/00479 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00592 , B01J2219/00599 , B01J2219/0065 , B01J2219/00657 , B01J2219/00664 , B01J2219/00702 , B01J2219/0072 , B01J2219/00722 , B01J2219/0074 , B01J2219/00743 , B01L3/502761 , B01L7/52 , B01L2200/027 , B01L2300/0636 , B01L2300/0645 , B01L2300/0654 , B01L2300/0681 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , C40B40/04 , C40B50/08 , G01N15/1459 , G01N33/5008 , G01N2015/1006 , G01N2500/00 , Y02A90/26
Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.
Abstract translation: 本发明一般涉及液滴库以及用于形成液滴库的系统和方法。 本发明还涉及在各种生物，化学或诊断测定中利用这些液滴文库的方法。
-
公开(公告)号：US20160201125A1
公开(公告)日：2016-07-14
申请号：US14995744
申请日：2016-01-14
Applicant: Raindance Technologies, Inc.
Inventor： Michael L. Samuels , Jeffrey Charles Olson , Andrew Watson , Keith Brown , Darren R. Link
IPC: C12Q1/68
Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
Abstract translation: 本发明提供条形码文件库及其制备和使用方法，包括获得多个核酸构建体，其中每个构建体包含独特的N-聚体和功能性N-聚体，并将构建体分离成流体隔室，使得每个隔室含有一个 或更多的独特构造的副本。 本发明还提供数字PCR和数字PCR中条形码库的使用方法。
-
公开(公告)号：US09127310B2
公开(公告)日：2015-09-08
申请号：US14264686
申请日：2014-04-29
Applicant: Raindance Technologies, Inc.
Inventor： Jonathan William Larson , Qun Zhong , Darren R. Link
CPC classification number: C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6883 , C12Q1/6886 , C12Q2600/172 , C12Q2563/159 , C12Q2565/629 , C12Q2537/143 , C12Q2531/113 , C12Q2535/131
Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.
Abstract translation: 本发明一般涉及基于液滴的数字PCR和使用其进行目标核酸分析的方法。 在某些实施方案中，本发明的方法包括形成含有平均单个靶核酸的样品液滴，扩增液滴中的靶，不包含来自靶标的扩增子的液滴和来自靶标变体的扩增子，并分析靶扩增子 。
-
公开(公告)号：US20150099266A1
公开(公告)日：2015-04-09
申请号：US14505974
申请日：2014-10-03
Applicant: Raindance Technologies, Inc.
Inventor： Michael Samuels , Jeffrey Olson , Darren R. Link
CPC classification number: G16B20/00
Abstract: In certain aspects, methods of the invention involve performing modification state specific enzymatic reaction of nucleic acid in a sample, determining a value associated with efficiency of the modification state specific enzymatic reaction based on a control, determining an amount of target nucleic acid in the sample, and normalizing the amount of target nucleic acid based on the efficiency value. Based on the normalized amount of target nucleic acid, the method further includes determining whether the normalized amount of target nucleic acid is indicative of a condition.
Abstract translation: 在某些方面，本发明的方法涉及在样品中进行核酸的修饰状态特异性酶反应，基于对照确定与修饰状态特异性酶促反应的效率相关的值，确定样品中靶核酸的量 ，并根据效率值使目标核酸的量归一化。 基于目标核酸的归一化量，该方法还包括确定目标核酸的归一化量是否指示条件。
-
Patent Agency Ranking
公开(公告)号：US08857462B2
公开(公告)日：2014-10-14
申请号：US14021481
申请日：2013-09-09
Applicant: Raindance Technologies, Inc.
Inventor： Benjamin Miller , Brian Hutchison , Andrew Wilson , Jonathan Larson , Qun Zhong , Yevgeny Yurkovetsky , Darren Link , Mark Weary
CPC classification number: G01N1/38 , B01L3/0241 , B01L3/502715 , B01L3/502784 , B01L2200/061 , B01L2200/0673 , B01L2200/143 , B01L2300/0877 , F16K99/0001 , F16K2099/0084 , F17D3/01 , G01N27/44791 , G01N2021/0353 , Y10T137/2185 , Y10T137/7761 , Y10T137/8158 , Y10T137/8593 , Y10T436/118339
Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.
Abstract translation: 本发明提供了用于评估微流体通道内的微流体液滴的一个或多个预定特征或性质的方法，以及调节该通道内的一个或多个流体流速以使用反馈控制选择性地改变预定的微滴特性或特性。
-
公开(公告)号：US20140256595A1
公开(公告)日：2014-09-11
申请号：US14226482
申请日：2014-03-26
Applicant: RAINDANCE TECHNOLOGIES, INC.
Inventor： Darren R. Link , Laurent Boitard , Jeffrey Branciforte , Yves Charles , Gilbert Feke , John Q. Lu , David Marran , Ahmadali Tabatabai , Michael Weiner , Wolfgang Hinz , Jonathan M. Rothberg
IPC: C12Q1/68
CPC classification number: C12N15/1075 , B01F3/0807 , B01F13/0071 , B01F13/0076 , B01J19/0046 , B01J2219/00286 , B01J2219/00459 , B01J2219/00466 , B01J2219/00468 , B01J2219/00479 , B01J2219/005 , B01J2219/00576 , B01J2219/00599 , B01J2219/00702 , B01J2219/0072 , B01J2219/00743 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L3/565 , B01L7/52 , B01L2200/027 , B01L2200/0621 , B01L2200/0647 , B01L2200/0652 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0896 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/084 , B82Y5/00 , B82Y30/00 , C07K1/047 , C12Q1/6804 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q1/6876 , C12Q2525/161 , C12Q2561/119 , C12Q2563/103 , C12Q2563/107 , C12Q2563/159 , C12Q2565/537 , C12Q2565/629 , C12Q2600/16 , C40B30/04 , C40B40/04 , C40B50/08 , C40B60/08 , C40B60/12 , C40B70/00 , G01N21/6428 , G01N21/6445 , G01N21/6458 , G01N33/536 , G01N33/542 , G01N33/543 , G01N33/6845 , G01N33/6854 , G01N2500/10
Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.
-
公开(公告)号：US20180135117A1
公开(公告)日：2018-05-17
申请号：US15863496
申请日：2018-01-05
Applicant: Raindance Technologies, Inc.
Inventor： Darren Roy Link
IPC: C12Q1/6848 , C12Q1/6806 , C12Q1/686 , B01L3/00
CPC classification number: C12Q1/6848 , B01F3/0807 , B01F13/0062 , B01J19/0093 , B01J2219/00783 , B01J2219/00788 , B01J2219/00828 , B01J2219/00831 , B01J2219/00833 , B01J2219/00837 , B01J2219/00853 , B01J2219/0086 , B01J2219/00869 , B01J2219/00889 , B01J2219/00903 , B01J2219/0097 , B01J2219/00988 , B01L3/502715 , B01L3/502746 , B01L3/502753 , B01L3/502776 , B01L3/502784 , B01L3/565 , B01L7/525 , B01L9/527 , B01L2200/027 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2200/141 , B01L2300/0636 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/165 , B01L2400/0415 , B01L2400/0424 , B01L2400/0487 , B01L2400/086 , B03C5/005 , B03C5/026 , B03C2201/26 , C12N15/1075 , C12Q1/00 , C12Q1/6806 , C12Q1/6827 , C12Q1/6844 , C12Q1/686 , C12Q1/6874 , C12Q2525/191 , C12Q2563/159 , C12Q2565/628 , C12Q2565/629 , G01N1/38 , G01N15/147 , G01N35/08 , G01N2035/00158 , G01N2035/00237 , G01N2035/00326 , C12Q2535/101 , C12Q2563/149
Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel
-
公开(公告)号：US20180100185A1
公开(公告)日：2018-04-12
申请号：US15835697
申请日：2017-12-08
Applicant: Raindance Technologies, Inc.
Inventor： Michael Samuels
IPC: C12Q1/686
Abstract: The invention generally relates to methods for increasing the amount of DNA available for analysis when using partitioned samples and parallel processing. For example, double-stranded DNA can be dissociated into two single-stranded components, and the single strands partitioned into different droplets prior to analysis. The disclosed methods are useful for performing digital PCR analysis on samples where the target DNA is not in abundance, for example when the sample originates from a body fluid or an FFPE sample.
-
公开(公告)号：US20170336306A1
公开(公告)日：2017-11-23
申请号：US15674074
申请日：2017-08-10
Applicant: Raindance Technologies, Inc.
Inventor： Benjamin J. Miller , Brian Hutchison , Andrew Wilson , Jonathan William Larson , Qun Zhong , Yevgeny Yurkovetsky , Darren Roy Link , Mark Weary
Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.
-
-
-
-
-
-
-
-
-
Search Results
167
records
(took 0.043 seconds)
