公开(公告)号：KR1020040102982A

公开(公告)日：2004-12-08

申请号：KR1020030034844

申请日：2003-05-30

Applicant: (주)엠젠 ,  박광욱

Inventor： 박광욱 ,  설재구 ,  허기남 ,  이승현 ,  박창식

IPC: C12N5/16

CPC classification number: C12N15/8778 ,  C12N15/85

Abstract: PURPOSE: A method for producing porcine clonal somatic cell line expressing green fluorescent protein is provided, which method allows for observation of differentiation and development of introduced cells at a cell therapy. CONSTITUTION: The method for producing porcine clonal somatic cell line expressing green fluorescent protein comprises the steps of: (1) isolating a somatic cell from a fetal pig; (2) producing an expression vector, pEGFP-C1 or PCX-EGFP, containing a fluorescent protein gene and cloning the expression vector into the somatic cell; and (3) selecting the expression vector cloned somatic cell and culturing the selected clonal somatic cell, wherein the fetal pig is 30 to 40 day of pregnancy pig; the fluorescent protein is EGFP(enhanced GFP); and the porcine clonal somatic cell line is PCX-8(KCLRF-BP-00079).

Abstract translation: 目的：提供一种生产表达绿色荧光蛋白的猪克隆体细胞系的方法，该方法允许观察细胞治疗引入细胞的分化和发育。 构成：产生表达绿色荧光蛋白的猪克隆体细胞系的方法包括以下步骤：（1）从胎猪中分离体细胞; （2）产生含有荧光蛋白基因的表达载体pEGFP-C1或PCX-EGFP，并将表达载体克隆入体细胞; （3）选择克隆体细胞的表达载体并培养选定的克隆体细胞，其中胎猪为妊娠猪30〜40天; 荧光蛋白是EGFP（增强GFP）; 猪克隆体细胞系为PCX-8（KCLRF-BP-00079）。