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公开(公告)号：KR101437545B1
公开(公告)日：2014-09-11
申请号：KR1020120132027
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 흑염소 반추위 미생물 유래의 섬유소분해효소 cel-KG36 유전자 및 이의 용도에 관한 것으로, 보다 구체적으로 흑염소 반추위 미생물로부터 선별한 신규한 섬유소분해효소 유전자인 cel-KG36 유전자 및 이의 단백질 산물을 제공하고, 이를 이용하여 사료첨가제, 세제 조성물 및 바이오연료를 제조하는 방법에 관한 것이다.
본 발명에 따르면, 흑염소 반추위 미생물로부터 신규한 섬유소분해효소 유전자인 cel-KG36 유전자를 선별할 수 있으며, 이를 통해 섬유소분해효소 cel-KG36 유전자 및 단백질 산물을 제공함으로써 저가의 사료 첨가제 개발에 이용할 수 있고, 섬유 유연제 및 세제 개발뿐만 아니라 풍부한 자원인 섬유소를 분해하여 바이오연료의 생산에도 적극적으로 이용할 수 있다.-
公开(公告)号：KR101437571B1
公开(公告)日：2014-09-05
申请号：KR1020120131957
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 흑염소 반추위 미생물 유래의 섬유소분해효소 cel-KG11 유전자 및 이의 용도에 관한 것으로, 보다 구체적으로 흑염소 반추위 미생물로부터 선별한 신규한 섬유소분해효소 유전자인 cel-KG11 유전자 및 이의 단백질 산물을 제공하고, 이를 이용하여 사료첨가제, 세제 조성물 및 바이오연료를 제조하는 방법에 관한 것이다. 본 발명에 따르면, 흑염소 반추위 미생물로부터 신규한 섬유소분해효소 유전자인 cel-KG11 유전자를 선별할 수 있으며, 이를 통해 섬유소분해효소 cel-KG11 유전자 및 단백질 산물을 제공함으로써 저가의 사료 첨가제 개발에 이용할 수 있고, 섬유 유연제 및 세제 개발뿐만 아니라 풍부한 자원인 섬유소를 분해하여 바이오연료의 생산에도 적극적으로 이용할 수 있다.
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23.发明公开
公开(公告)号：KR1020140064576A
公开(公告)日：2014-05-28
申请号：KR1020120132030
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG49 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG49 gene which is a novel fibrinogenase selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG49 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG49 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及一种来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG49基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶的cel-KG49基因， 通过使用它们提供蛋白质产物和制造饲料添加剂，洗涤剂组合物和生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG49基因，通过提供纤维蛋白原酶cel-KG49基因和蛋白质产物，可以用于显影 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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公开(公告)号：KR1020140064571A
公开(公告)日：2014-05-28
申请号：KR1020120132022
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG25 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG25 gene which is a novel fibrinogenase selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, it is possible to select a cel-KG25 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat, and by providing the fibrinogenase cel-KG25 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及来源于黑山羊瘤胃微生物的纤维蛋白原酶cel-KG25基因及其用途，更具体地说，涉及一种选自黑山羊的瘤胃微生物的新型纤维蛋白原酶的cel-KG25基因， 通过使用它们提供蛋白质产物和制造饲料添加剂，洗涤剂组合物和生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG25基因，通过提供纤维蛋白原酶-KK25基因和蛋白质产物，可以使用它们 用于开发低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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25.发明公开
公开(公告)号：KR1020140064565A
公开(公告)日：2014-05-28
申请号：KR1020120132009
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG48 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG48 gene which is a novel fibrinogenase gene selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG48 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG48 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG48基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶基因cel-KG48基因 提供其蛋白质产物和制造饲料添加剂，洗涤剂组合物和使用它们的生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG48基因，通过提供纤维蛋白原酶cel-KG48基因和蛋白质产物，可以用于显影 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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Patent Agency Ranking26.发明公开
公开(公告)号：KR1020140064558A
公开(公告)日：2014-05-28
申请号：KR1020120131997
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG43 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG43 gene which is a novel fibrinogenase gene selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG43 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG43 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及一种来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG43基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶基因cel-KG43基因 提供其蛋白质产物和制造饲料添加剂，洗涤剂组合物和使用它们的生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG43基因，通过提供纤维蛋白原酶cel-KG43基因和蛋白质产物，可以用于显影 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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27.发明公开
公开(公告)号：KR1020140064544A
公开(公告)日：2014-05-28
申请号：KR1020120131973
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG33 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG33 gene which is a novel fibrinogenase selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, it is possible to select a cel-KG33 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat, and by providing the fibrinogenase cel-KG33 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG33基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶的cel-KG33基因， 通过使用它们提供蛋白质产物和制造饲料添加剂，洗涤剂组合物和生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG33基因，通过提供纤维蛋白原酶cel-KG33基因和蛋白质产物，可以使用它们 用于开发低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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公开(公告)号：KR1020130068007A
公开(公告)日：2013-06-25
申请号：KR1020110135113
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/248 , A23K10/14 , C11D3/38636 , C12N15/67 , C12P7/649
Abstract: PURPOSE: A product of cel10-KG41 gene, endoxylanase, is provided to be used for a feed additive and a detergent composition, and to develop biofuel with high efficiency. CONSTITUTION: An endoxylanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes endoxylanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoxylanase from the host cell comprises a step of overexpressing endoxylanase by transducing the recombinant vector to the host cells.
Abstract translation: 目的：提供cel10-KG41基因（内切木聚糖酶）的产品，用于饲料添加剂和洗涤剂组合物，并以高效率开发生物燃料。 构成：源自黑山羊的反刍动物胃微生物的内切木聚糖酶蛋白具有序列号2的氨基酸序列。基因编码内切木聚糖酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量内切木聚糖酶的方法包括通过将重组载体转导至宿主细胞而过表达内切木聚糖酶的步骤。
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公开(公告)号：KR1020130068006A
公开(公告)日：2013-06-25
申请号：KR1020110135112
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2445 , A23K10/14 , C07K16/40 , C11D3/386 , C12N15/67 , C12P7/649 , C12Y302/01021 , Y02E50/13
Abstract: PURPOSE: A protein of cel3-KG39 gene, beta-glucosidase, which is acquired by searching glucosidase gene from a ruminant stomach microorganism of a black goat is provided to be used for a feed additive and a detergent composition, and to develop biofuel. CONSTITUTION: A beta-glucosidase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes beta-glucosidase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of beta-glucosidase in the host cell comprises: a step of transducing the recombinant vector to the host cell; and a step of overexpressing beta-glucosidase. [Reference numerals] (A) Front domain area; (B) Rear domain area
Abstract translation: 目的：提供通过从黑山羊的反刍动物胃微生物中搜索葡糖苷酶基因获得的cel3-KG39基因的β-葡萄糖苷酶蛋白，用于饲料添加剂和洗涤剂组合物，并开发生物燃料。 构成：来自黑山羊的反刍动物胃微生物的β-葡糖苷酶蛋白具有序列号2的氨基酸序列。基因编码β-葡糖苷酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 用于在宿主细胞中产生大量β-葡糖苷酶的方法包括：将重组载体转导至宿主细胞的步骤; 以及过表达β-葡糖苷酶的步骤。 （附图标记）（A）前域区域; （B）后域区域
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公开(公告)号：KR1020130068005A
公开(公告)日：2013-06-25
申请号：KR1020110135111
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2437 , A23K10/14 , C11D3/38645 , C12N15/67 , C12P1/00 , Y02E50/10
Abstract: PURPOSE: A product of cel-KG35 gene, endoglucanase, is provided to be used for a feed additive and a detergent composition, and to develop biofuel with high efficiency. CONSTITUTION: An endoglucanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes the endoglucanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoglucanase in the host cell comprises: a step of transducing the recombinant vector to the host cell; and a step of overexpressing endoglucanase.
Abstract translation: 目的：提供cel-KG35基因，内切葡聚糖酶的产品，用于饲料添加剂和洗涤剂组合物，并以高效率开发生物燃料。 构成：来自黑山羊的反刍动物胃微生物的内切葡聚糖酶蛋白具有序列号2的氨基酸序列。基因编码内切葡聚糖酶蛋白质。 重组载体含有该基因。 宿主细胞被重组载体转化。 用于在宿主细胞中产生大量内切葡聚糖酶的方法包括：将重组载体转导至宿主细胞的步骤; 以及过表达内切葡聚糖酶的步骤。
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