公开(公告)号：KR101410329B1

公开(公告)日：2014-06-27

申请号：KR1020120106892

申请日：2012-09-26

Applicant: 서울대학교산학협력단

Inventor： 강병철 ,  응후이 ,  양희범 ,  최도일

IPC: C12N15/11 ,  C12Q1/68

Abstract: 본 발명은 TSWV 저항성 고추 품종을 선별하기 위한 프라이머 세트, 방법 및 키트에 관한 것으로, 더욱 상세하게는 본 발명에 따른 프라이머 세트를 고추 작물들에 적용하여 TSWV에 저항성을 나타내는 고추 품종을 효율적으로 선별 및 육성할 수 있게 하는 기반기술을 제공하고자 하는 것이다.

公开(公告)号：KR1020130044620A

公开(公告)日：2013-05-03

申请号：KR1020110108767

申请日：2011-10-24

Applicant: 서울대학교산학협력단

Inventor： 염선인 ,  서은영 ,  오상근 ,  최도일

IPC: A01H5/00 ,  C07K14/415 ,  C12N15/29 ,  C12N15/82

CPC classification number: C07K14/415 ,  A01H4/008 ,  C12N15/8205 ,  C12N15/8216 ,  C12N15/8263 ,  C12N15/8279

Abstract: PURPOSE: A plant cell wall protein HyPRP1(hybrid proline-rich protein-1) coding gene is provided to develop a transgenic plant with enhanced cell apoptosis regulation and disease resistance, and to enhance resistance to pathogens. CONSTITUTION: Plant cell wall protein, HyPRP1, which regulates cell apoptosis or disease resistance, contains an amino acid sequence in sequence number 2 or 4. A gene encodes the protein, and contains a base sequence in sequence number 1 or 3. A recombinant vector contains the gene. A host cell is transformed with the recombinant vector.

Abstract translation: 目的：提供植物细胞壁蛋白HyPRP1（杂交脯氨酸富含蛋白-1）编码基因，以开发具有增强的细胞凋亡调节和抗病性的转基因植物，并增强对病原体的抗性。 构成：调节细胞凋亡或抗病性的植物细胞壁蛋白HyPRP1含有序列号2或4中的氨基酸序列。基因编码蛋白质，并含有序列号1或3中的碱基序列。重组载体 含有该基因。 用重组载体转化宿主细胞。

公开(公告)号：KR1020120108528A

公开(公告)日：2012-10-05

申请号：KR1020110026510

申请日：2011-03-24

Applicant: 서울대학교산학협력단

Inventor： 최도일 ,  이동주

IPC: A01H5/00 ,  C12N15/29 ,  C12N15/82 ,  C12N5/04

CPC classification number: C12N15/8261 ,  C07K14/415 ,  C12N15/8205 ,  C12N15/8216 ,  C12N15/8266

Abstract: PURPOSE: A composition containing Capsicum anuum-derived CaRLK1 gene for promoting plant cell division and proliferating cells or suppressing aging is provided to be used in pharmaceutical products and cosmetic products. CONSTITUTION: A composition for promoting plant cells division and proliferating cells contains a gene encoding CaRLK1(Capsicum annuum Receptor-like Kinase 1) protein containing an amino acid of sequence number 2 as an active ingredient. A composition for suppressing plant cell aging contains the gene. The gene has a base sequence of sequence number 1. A recombinant vector for intracellular transduction contains the gene. A method for proliferating cells and promoting plant cells division comprises a step of transforming the recombinant vector to a plant and a step of overexpressing the gene.

Abstract translation: 目的：提供用于促进植物细胞分裂和增殖细胞或抑制衰老的含有辣椒来源的CaRLK1基因的组合物用于药物和化妆品。 构成：用于促进植物细胞分裂和增殖细胞的组合物含有编码含有序列号2的氨基酸作为活性成分的CaRLK1（辣椒真菌受体样激酶1）蛋白的基因。 抑制植物细胞衰老的组合物含有该基因。 该基因具有序列号1的碱基序列。用于细胞内转导的重组载体含有该基因。 增殖细胞和促进植物细胞分裂的方法包括将重组载体转化到植物和过表达该基因的步骤。

公开(公告)号：KR1020100090153A

公开(公告)日：2010-08-13

申请号：KR1020090009472

申请日：2009-02-05

Applicant: 서울대학교산학협력단

Inventor： 최도일 ,  오상근 ,  김샛별

IPC: C12N15/66 ,  C12N15/82

Abstract: PURPOSE: A ligation independent cloning vector for transient plant expression is provided to easily quickly perform cloning and to redue cost. CONSTITUTION: A ligation independent cloning vector comprises right adapter, SnaBI recognition site, ccdB1(control of cell death B1), SnaBI recognition site, left adapter, and haemagglutinin gene from 5' to 3'. The vector is pJL-CLIC vector. A method for cloning ligation independence comprises: a step of cleaving ligation independent cloning vector with SnaBI restriction vector; a step of treating with T4 DNA polymerase; and a step of mixing the treated reactant. A host cell transformed by a vector is Agrobacterium.

Abstract translation: 目的：提供用于瞬时植物表达的连接独立克隆载体，以方便快速进行克隆并降低成本。 构成：连接独立克隆载体包括正确的适配器，SnaBI识别位点，ccdB1（细胞死亡B1的控制），SnaBI识别位点，左转座和5'至3'的血细胞凝集素基因。 载体是pJL-CLIC载体。 用于克隆连接独立性的方法包括：用SnaBI限制载体切割连接独立克隆载体的步骤; 用T4 DNA聚合酶处理的步骤; 以及混合经处理的反应物的步骤。 由载体转化的宿主细胞是农杆菌。