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公开(公告)号：KR1020120054119A
公开(公告)日：2012-05-30
申请号：KR1020100115328
申请日：2010-11-19
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
IPC: A01K67/027 , C12N15/867 , C12N15/65
CPC classification number: A01K67/027 , C12N15/65 , C12N15/867
Abstract: PURPOSE: The production of transgenic aves using sequences for germ cell-specific gene expression is provided to analyze the differentiation or development of the priordial germ cells of aves into germ cells. CONSTITUTION: A method for producing transgenic aves includes the following: the priordial germ cells of aves are transformed based on a genetic vector containing a DAZL promoter as a germ cell-specific promoter, a NANOG promoter as a stem cell-specific promoter, and nucleotide sequence which encodes protein of revelation purpose; the transformed priordial germ cells of the aves are injected into accept media; and the transformed product of the aves is obtained. The protein is operatively bonded to the promoters.
Abstract translation: 目的：提供使用生殖细胞特异性基因表达序列的转基因抗生素的生产，以分析生物细胞中二次生殖细胞的分化或发育。 构成：用于生产转基因鸟类的方法包括以下：基于含有作为生殖细胞特异性启动子的DAZL启动子，作为干细胞特异性启动子的NANOG启动子和核苷酸的核苷酸的遗传载体转化aves的无性繁殖细胞 编码启示目的蛋白的序列; 将转化的雄性生殖细胞注入接受培养基; 并获得aves的转化产物。 蛋白质可操作地结合到启动子上。
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公开(公告)号：KR1020150064300A
公开(公告)日：2015-06-11
申请号：KR1020130148677
申请日：2013-12-02
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
IPC: C12N15/12 , C12N15/85 , A01K67/027
Abstract: 본발명은코돈최적화된인간상피세포성장인자를발현하는형질전환조류및 이의제조방법에관한것이다. 본발명에따른인간상피세포성장인자를발현하는형질전환된조류는인간상피세포성장인자가난관특이적으로발현되어난백내에축적됨으로써효율적으로상피세포성장인자를생산할수 있다.
Abstract translation: 本发明涉及用于表达密码子优化的人表皮生长因子的转基因Aves和用于制备转基因Aves的方法。 根据本发明，表达密码子优化的人表皮生长因子的转基因Aves具有在输卵管上特异性表达以沉积在蛋清中的人表皮生长因子，从而有效地产生表皮生长因子。
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公开(公告)号：KR1020040073174A
公开(公告)日：2004-08-19
申请号：KR1020030009167
申请日：2003-02-13
Applicant: 주식회사 옵티팜
IPC: C12N15/00
Abstract: PURPOSE: A method for isolating gonadal primordial germ cells and a method for preparing germline chimeric aves are provided, thereby improving purity and isolation yield of gonadal primordial germ cells, and improving preparation efficiency of germline chimeric aves. CONSTITUTION: The method for isolating gonadal primordial germ cells comprises the steps of: (a) treating gonadal primordial germ tissue cells isolated from embryos of aves with a primordial germ cell-specific first antibody; (b) treating the antibody-bound gonadal primordial germ tissue cells with microbeads bound with a second antibody which has magnetism and binds with the first antibody; and (c) cleansing the resulting product of step (b) and separating the gonadal primordial germ cell-second antibody-microbead complex. The method for preparing germline chimeric aves comprises the steps of: (a) treating gonadal primordial germ tissue cells isolated from embryos of aves with a primordial germ cell-specific first antibody; (b) treating the antibody-bound gonadal primordial germ tissue cells with microbeads bound with a second antibody which has magnetism and binds with the first antibody; (c) cleansing the resulting product of step (b) and separating the gonadal primordial germ cell-second antibody-microbead complex; (d) introducing the isolated gonadal primordial germ cells into the embryo of a recipient egg; and (e) culturing and hatching the egg.
Abstract translation: 目的：提供一种分离性腺原始生殖细胞的方法和制备种系嵌合抗体的方法，从而提高性腺原始生殖细胞的纯度和分离产量，提高种系嵌合抗体的制备效率。 构成：分离性腺原始生殖细胞的方法包括以下步骤：（a）用原始生殖细胞特异性第一抗体处理从禽类胚胎分离的性腺原始胚芽组织细胞; （b）用与具有磁性并与第一抗体结合的第二抗体结合的微珠处理抗体结合的性腺原始细菌组织细胞; 和（c）清洗步骤（b）的所得产物并分离性腺原始生殖细胞 - 第二抗体 - 微珠复合物。 用于制备种系嵌合抗体的方法包括以下步骤：（a）用原始生殖细胞特异性第一抗体处理从禽类胚胎分离的性腺原始胚芽组织细胞; （b）用与具有磁性并与第一抗体结合的第二抗体结合的微珠处理抗体结合的性腺原始细菌组织细胞; （c）清洗步骤（b）的所得产物并分离性腺原始生殖细胞 - 第二抗体 - 微珠复合物; （d）将分离的性腺原始生殖细胞引入受体卵的胚胎; 和（e）培养和孵化蛋。
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24.发明公开조류 콕시듐증을 유발하는 에이메리아 종의 포자소체 표면항원에 대한 ｓｃＦｖ 재조합 항체 失效特异性针对EIMERIA SPP的SPOROZOITE SURFACE抗原的重组SCFV抗体。 负责治疗
公开(公告)号：KR1020030018684A
公开(公告)日：2003-03-06
申请号：KR1020010052934
申请日：2001-08-30
Applicant: 주식회사 옵티팜
IPC: C07K14/455
CPC classification number: C07K16/20 , C07K2317/622 , Y10S530/822
Abstract: PURPOSE: Recombinant ScFv antibodies specific to a sporozoite surface antigen of Eimeria spp. responsible for coccidiosis are provided. The recombinant ScFv antibodies has improved tissue-penetrating activity, can be mass-produced from a prokaryotic host cell such as Escherichia coli, and can be used in passive immunization of coccidiosis. CONSTITUTION: The variable regions of heavy chains in antibodies specific to the sporozoite surface antigen of Eimeria spp. have the amino acid sequence selected from SEQ ID NOs: 18, 20, 22, 24 and 38. The variable regions of light chains in antibodies specific to the sporozoite surface antigen of Eimeria spp. have the amino acid sequence selected from SEQ ID NOs: 26, 28, 30, 32 and 40. The genes encoding the variable regions of heavy chains in antibodies specific to the sporozoite surface antigen of Eimeria spp. having the amino acid sequence selected from SEQ ID NOs: 18, 20, 22, 24 and 38 have the nucleotide sequence selected from SEQ ID NOs: 17, 19, 21, 23 and 37. The genes encoding the variable regions of light chains in antibodies specific to the sporozoite surface antigen of Eimeria spp. having the amino acid sequence selected from SEQ ID NOs: 26, 28, 30, 32 and 40 have the nucleotide sequence selected from SEQ ID NOs: 25, 27, 29, 31 and 39.
Abstract translation: 目的：针对艾美球虫属的子孢子表面抗原特异的重组ScFv抗体。 负责球虫病的提供。 重组ScFv抗体具有改善的组织穿透活性，可以从原核宿主细胞如大肠杆菌大量生产，并可用于球虫病的被动免疫。 构成：艾美球虫亚种子孢子表面抗原特异抗体的重链可变区。 具有选自SEQ ID NO：18,20,22,24和38的氨基酸序列。特异于艾美球虫属的子孢子表面抗原的抗体中的轻链的可变区。 具有选自SEQ ID NO：26,28,30,32和40的氨基酸序列。编码艾美球虫属的子孢子表面抗原特异性抗体的重链可变区的基因。 具有选自SEQ ID NO：18,20,22,24和38的氨基酸序列具有选自SEQ ID NO：17,19,21,23和37的核苷酸序列。编码轻链的可变区的基因 对艾美球虫亚种子孢子表面抗原特异的抗体。 具有选自SEQ ID NO：26,28,30,32和40的氨基酸序列具有选自SEQ ID NO：25,27,29,31和39的核苷酸序列。
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公开(公告)号：KR101574204B1
公开(公告)日：2015-12-04
申请号：KR1020130148677
申请日：2013-12-02
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
IPC: C12N15/12 , C12N15/85 , A01K67/027
Abstract: 본발명은코돈최적화된인간상피세포성장인자를발현하는형질전환조류및 이의제조방법에관한것이다. 본발명에따른인간상피세포성장인자를발현하는형질전환된조류는인간상피세포성장인자가난관특이적으로발현되어난백내에축적됨으로써효율적으로상피세포성장인자를생산할수 있다.
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Patent Agency Ranking
公开(公告)号：KR101423971B1
公开(公告)日：2014-08-04
申请号：KR1020100115328
申请日：2010-11-19
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
IPC: A01K67/027 , C12N15/867 , C12N15/65
Abstract: 본 발명은 NANOG 프로모터 또는 DAZL 프로모터-목적 단백질 인코딩 서열의 컨스트럭트를 이용하여 형질전환 조류를 최초로 구축한 것이다. 본 발명은 조류 원시생식세포로부터 생식세포로의 분화 또는 발달 연구에 매우 유용한 툴을 제공한다. 본 발명의 형질전환 조류를 이용하면, 생식세포 발달과정 동안 전능성의 조절 기전을 규명할 수 있고, 또한 생식세포 특정화(germ cell specification)가 조류서 어느 시기에 이루어지는지 규명할 수 있다.
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公开(公告)号：KR100490669B1
公开(公告)日：2005-05-19
申请号：KR1020010052934
申请日：2001-08-30
Applicant: 주식회사 옵티팜
IPC: C07K14/455
CPC classification number: C07K16/20 , C07K2317/622 , Y10S530/822
Abstract: 본 발명은 조류 콕시듐증을 유발하는 에이메리아 종 (
Eimeria spp. )의 포자소체 표면 항원에 대한 신규한 scFv 항체에 관한 것으로서, 보다 상세하게는 본 발명은 scFv 재조합 항체의 제조에 특히 적합한 신규한 중사슬 및 경사슬의 가변성 부위, 이를 이용한 scFv 재조합 항체에 관한 것으로서, 본 발명의 scFv 재조합 항체는 항체로서의 기능을 충분히 발휘하면서도, 그 작은 크기 때문에 우수한 조직 투과 특성을 나타내고, 대장균과 같은 원핵세포 숙주를 통하여 대량으로 얻을 수 있으며, 조류 콕시듐증의 수동적 면역화에 이용될 수 있고, 에이메리아 백신 항원에 대한 친화성 정제에 이용될 수 있다.-
公开(公告)号：KR100470170B1
公开(公告)日：2005-02-04
申请号：KR1020030009167
申请日：2003-02-13
Applicant: 주식회사 옵티팜
IPC: C12N15/00
Abstract: PURPOSE: A method for isolating gonadal primordial germ cells and a method for preparing germline chimeric aves are provided, thereby improving purity and isolation yield of gonadal primordial germ cells, and improving preparation efficiency of germline chimeric aves. CONSTITUTION: The method for isolating gonadal primordial germ cells comprises the steps of: (a) treating gonadal primordial germ tissue cells isolated from embryos of aves with a primordial germ cell-specific first antibody; (b) treating the antibody-bound gonadal primordial germ tissue cells with microbeads bound with a second antibody which has magnetism and binds with the first antibody; and (c) cleansing the resulting product of step (b) and separating the gonadal primordial germ cell-second antibody-microbead complex. The method for preparing germline chimeric aves comprises the steps of: (a) treating gonadal primordial germ tissue cells isolated from embryos of aves with a primordial germ cell-specific first antibody; (b) treating the antibody-bound gonadal primordial germ tissue cells with microbeads bound with a second antibody which has magnetism and binds with the first antibody; (c) cleansing the resulting product of step (b) and separating the gonadal primordial germ cell-second antibody-microbead complex; (d) introducing the isolated gonadal primordial germ cells into the embryo of a recipient egg; and (e) culturing and hatching the egg.
Abstract translation: 目的：提供分离性腺原始生殖细胞的方法和制备种系嵌合体的方法，从而提高性腺原始生殖细胞的纯度和分离产率，并提高种系嵌合体的制备效率。 构成：分离性腺原始生殖细胞的方法包括以下步骤：（a）用原始生殖细胞特异性第一抗体处理从雏鸟胚胎分离的性腺原始生殖细胞组织细胞; （b）用具有磁性并与第一抗体结合的第二抗体结合的微珠处理结合抗体的性腺原始生殖细胞组织细胞; 和（c）清洗步骤（b）的所得产物并分离性腺原始生殖细胞 - 第二抗体 - 微珠复合物。 用于制备种系嵌合体的方法包括以下步骤：（a）用原始生殖细胞特异性第一抗体处理从雏鸟胚胎分离的性腺原始生殖细胞组织细胞; （b）用具有磁性并与第一抗体结合的第二抗体结合的微珠处理结合抗体的性腺原始生殖细胞组织细胞; （c）清洗步骤（b）的所得产物并分离生殖细胞原始生殖细胞 - 第二抗体 - 微珠复合物; （d）将分离的性腺原始生殖细胞引入受体卵的胚胎中; 和（e）培养和孵化鸡蛋。
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公开(公告)号：KR1020040051765A
公开(公告)日：2004-06-19
申请号：KR1020020079428
申请日：2002-12-13
Applicant: 주식회사 옵티팜
IPC: C12N5/16
CPC classification number: C12N15/8771 , A01K67/0273 , A01K2227/101 , A61D19/04 , C12N5/16 , C12N13/00 , C12N2510/04 , C12N2517/00
Abstract: PURPOSE: A method for inactivating nuclear chromosomal materials of recipient oocyte for manufacturing clone embryo from somatic cell using X-ray irradiation is provided, thereby increasing a blastocyst development rate and replacing the physical nucleus removing method. CONSTITUTION: The method for inactivating nuclear chromosomal materials of recipient oocyte for manufacturing clone embryo from somatic cell comprises irradiating X-ray to the recipient oocyte. The method for producing somatic cell cloned animals comprises the steps of: (a) irradiating X-ray to the recipient oocyte; (b) transplanting the somatic cell donor nucleus into the recipient oocyte; (c) inducing the re-programming of the nucleus transplanted somatic cell cloned embryo; (d) inducing the activity of the re-programmed somatic cell cloned embryo; (e) inducing the development of blastocyst of the activated somatic cell cloned embryo; and (f) inducing the implantation and generation of blastocyst, wherein the X-ray irradiation is carried out at 15-18 kVp; the X-ray irradiation is carried out at 2-8 mA; and the X-ray irradiation is carried out for 10-60 seconds.
Abstract translation: 目的：提供使用X射线照射从体细胞制备克隆胚胎的受体卵母细胞核染色体材料灭活的方法，从而提高胚泡发育速度并取代物理核去除方法。 构成：从体细胞中制备克隆胚胎的受体卵母细胞的核染色体材料的灭活方法包括向受体卵母细胞照射X射线。 产生体细胞克隆动物的方法包括以下步骤：（a）向受体卵母细胞照射X射线; （b）将体细胞供体核移植到受体卵母细胞中; （c）诱导细胞移植体细胞克隆胚胎的重新编程; （d）诱导重编程体细胞克隆胚胎的活性; （e）诱导活化的体细胞克隆胚胎的胚泡的发育; 和（f）诱导胚泡的植入和产生，其中X射线照射在15-18kVp下进行; X射线照射在2-8mA进行; 并进行X射线照射10〜60秒。
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公开(公告)号：KR101232850B1
公开(公告)日：2013-02-22
申请号：KR1020100115395
申请日：2010-11-19
Applicant: 서울대학교산학협력단 , 주식회사 옵티팜
Abstract: 본 발명은 서열번호 제1서열 내지 제5서열로 구성된 군으로부터 선택되는 뉴클레오타이드 서열에 특이적으로 결합하는 결합제를 포함하는 생식관의 발생 또는 분화 분석용 키트 및 이를 이용한 스크리닝 방법에 관한 것이다. 본 발명자들은 닭의 난관에 합성 에스트로겐, 바람직하게는 디에틸스틸베스트롤(diethylstilbestrol, DES)을 처리하여 실시한 전사체 프로파일(transcriptional profiling) 분석을 통해 생식관(바람직하게는, 난관) 발생 또는 분화-특이적 신규한 바이오마커인
CCRN4L (carbon catabolite repression 4-like),
NTM (neurotrimin),
HAS2 (hyaluronan synthase 2),
NELF (nasal embryonic LHRH factor) 및
FAM26F (family with sequence similarity 26, member F) 유전자를 동정하였다. 따라서, 본 발명의 마커 및 방법은 닭의 난관 발생에 필요한 유전자 분화 및 발생을 동정하기 위한 난관의 유전적 조절에 대한 새로운 시각을 제공한다.
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