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公开(公告)号：KR1020110055930A
公开(公告)日：2011-05-26
申请号：KR1020090112557
申请日：2009-11-20
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/56 , C12Q1/6834 , G01N29/48
Abstract: PURPOSE: A method and system for detecting thrombine using a micro-cantilever-based thrombine is provided to effectively detect a small amount of thrombine. CONSTITUTION: A system for detecting micro-cantilever sensor-based thrombin comprises: a micro-cantilever sensor, a DNA aptamer fixed on the micro-cantilever, and a device for measuring resonant frequency of the micro-cantilever. The DNA aptamer is single strand DNA aptamer, or DNA aptamer-g-DNA duplex structure. The micro-cantilever contains PZT(lead zirconate titanate) or foil layer. A method for detecting the thrombine comprises: a step of fixing the DNA aptamer to the micro-cantilever; a step of measuring the resonant frequency of the micro-cantilever; a step of reacting a bio sample with the micro-cantilever; a step of measuring resonant frequency of the micro-cantilever; and a step of calculating the variance value of before and after of the reaction.
Abstract translation: 目的：提供一种使用微悬臂梁血栓检测血栓的方法和系统，有效检测少量血栓素。 构成：用于检测基于微悬臂传感器的凝血酶的系统包括：微悬臂梁传感器，固定在微悬臂上的DNA适配体，以及用于测量微悬臂梁共振频率的装置。 DNA适体是单链DNA适配体，或DNA适体-g-DNA双链体结构。 微型悬臂包含PZT（钛酸铅锆石）或箔层。 检测血栓素的方法包括：将DNA适配体固定在微悬臂梁上的步骤; 测量微型悬臂的谐振频率的步骤; 将生物样品与微悬臂梁反应的步骤; 测量微悬臂梁的共振频率的步骤; 以及计算反应前后的方差值的步骤。
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公开(公告)号：KR101029343B1
公开(公告)日：2011-04-13
申请号：KR1020090070041
申请日：2009-07-30
Applicant: 한국과학기술연구원
IPC: G01N33/53 , G01N33/533 , G01N33/563 , C12Q1/68
CPC classification number: G01N33/542 , G01N33/54306 , G01N2458/10
Abstract: 포획항체, 단일가닥 DNA올리고뉴클레오타이드와 결합된 검출항체, 상기 DNA 올리고뉴클레오타이드와 상보적 서열을 갖고 형광물질로 표지된 단일가닥 RNA 올리고뉴클레오타이드, 및 RNA 분해효소를 포함하는 항원 검출용 키트; 및 이를 이용하는 항원 검출 방법이 제공된다.
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公开(公告)号：KR1020110012353A
公开(公告)日：2011-02-09
申请号：KR1020090070041
申请日：2009-07-30
Applicant: 한국과학기술연구원
IPC: G01N33/53 , G01N33/533 , G01N33/563 , C12Q1/68
CPC classification number: G01N33/542 , G01N33/54306 , G01N2458/10 , G01N33/533 , G01N33/563
Abstract: PURPOSE: A kit for detecting an antigen and a detection method using the same are provided to simultaneously detect several antigens by multiple-OLISA(oligonucleotide-linked immunosorbent assay). CONSTITUTION: A kit for detecting an antigen comprises: a support; a capture antibody(c-Ab) which is fixed on the support; a detection antibody-DNA conjugate(d-Ab-DNA); a fluorescence-RNA-quencher conjugate; and RNase H. The kit is able to detect two or more kinds of antigens. A method for detecting the antigen comprises: a step of contacting a sample to the capture antibody which is able to selectively conjugate to the antigen to be detected to conjugate the capture antibody and antigen in the sample; a step of conjugating the antigen with d-Ab-DNA; a step of contacting the antibody with fluorescence-RNA quencher conjugate to hybridize DNA and RNA; a step of treating with RNase H to cut RNA and release fluorescence from RNA; and a step of measuring fluorescence intensity.
Abstract translation: 目的：提供用于检测抗原的试剂盒和使用其的检测方法，以通过多次OLISA（寡核苷酸连接的免疫吸附测定）同时检测几种抗原。 构成：用于检测抗原的试剂盒包括：载体; 固定在载体上的捕获抗体（c-Ab）; 检测抗体-DNA缀合物（d-Ab-DNA）; 荧光-RNA-猝灭剂缀合物; 和RNA酶H.该试剂盒能够检测两种或多种抗原。 检测抗原的方法包括：使样品与能够选择性地与待检测的抗原缀合的捕获抗体与样品中的捕获抗体和抗原缀合的步骤; 将抗原与d-Ab-DNA缀合的步骤; 将抗体与荧光-RNA猝灭剂缀合物接触以使DNA和RNA杂交的步骤; 用RNase H处理RNA以从RNA中释放荧光的步骤; 以及测量荧光强度的步骤。
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公开(公告)号：KR1020080084029A
公开(公告)日：2008-09-19
申请号：KR1020070025105
申请日：2007-03-14
Applicant: 한국과학기술연구원
CPC classification number: G01N33/68 , C12N15/111 , C12N15/115 , C12N2310/16 , C12N2320/10 , C12Q1/682 , C12Q1/6823 , G01N2500/00 , C12Q2521/301 , C12Q2521/327 , C12Q2525/205 , C12Q2565/1015 , C12Q2525/161
Abstract: A method for detecting a target protein using a DNA aptamer is provided to be more convenient and economical than a conventional method using an antibody and be used for detecting the target protein with high sensitivity through amplification of the fluorescence intensity by repetitive action of RNase H. A method for detecting a target protein comprises the steps of: (a) binding a DNA aptamer coupled to the target protein to a D-GNA(guard-DNA) complementary to the aptamer to form a double-stranded DNA; (b) treating a single stranded RNA, which is complementary to the double-stranded DNA and the G-DNA and is coupled to a fluorophore and a quencher, with a sample including the target protein; (c) treating RNase H; and (d) measuring the intensity of the fluorescence in the sample. A kit for detecting a target protein comprises: a double stranded DNA consisting of a DNA aptamer coupled to the target protein and a G-DNA; a single stranded RNA coupled to a fluorophore and a quencher; and RNase H. Further, the DNA aptamer is 5'Ex_aptamer.
Abstract translation: 提供了使用DNA适体检测靶蛋白的方法比使用抗体的常规方法更方便和经济，并且通过RNA酶H的重复作用扩增荧光强度而用于高灵敏度检测靶蛋白。 用于检测靶蛋白的方法包括以下步骤：（a）将与靶蛋白偶联的DNA适配体与适配体互补的D-GNA（保护DNA）结合以形成双链DNA; （b）用包含靶蛋白的样品处理与双链DNA和G-DNA互补并与荧光团和猝灭剂偶联的单链RNA; （c）处理RNase H; 和（d）测量样品中的荧光强度。 用于检测靶蛋白的试剂盒包括：由与靶蛋白偶联的DNA适配体和G-DNA组成的双链DNA; 与荧光团和猝灭剂偶联的单链RNA; 另外，DNA适体是5'Ex_aptamer。
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25.发明公开ＨＩＦ-１α Ｃ-말단 펩타이드와 ＣＢＰ 또는 ｐ３００단백질과의 결합을 정량분석하는 방법 및 상기 방법을이용한 단백질 복합체 형성을 저해하는 억제제의 스크리닝방법 失效用于定量分析HIF-1ALPHA C端蛋白与CBP或P300蛋白质之间的相互作用的方法以及使用其形成蛋白复合物筛选抑制剂的方法
公开(公告)号：KR1020080033643A
公开(公告)日：2008-04-17
申请号：KR1020060099389
申请日：2006-10-12
Applicant: 한국과학기술연구원
CPC classification number: G01N33/542 , G01N33/533 , G01N2333/4703 , G01N2500/02 , G01N2800/40 , G01N2333/47
Abstract: A method for quantitative analysis of interactions between HIF-1alpha C-terminal peptides and CBP(cyclic AMP-response element-binding protein) or p300 proteins is provided to reduce the analysis costs by removal of separation for a protein complex of HIF-1alpha C-terminal peptides and CBP or p300 proteins and automation with well plate, screen a large quantity of inhibitors against the protein complex, and analyze efficacy of post-translational modification of HIF-1alpha. A method for quantitative analysis of interactions between HIF-1alpha C-terminal peptides and CBP or p300 proteins comprises the steps of: (1) attaching a fluorescent material to the HIF-1alpha C-terminal peptide to prepare a fluorescent probe; (2) contacting the fluorescence-labeled HIF-1alpha C-terminal peptide with CBP or p300 protein to react the fluorescent material with the CBP or p300; and (3) measuring fluorescence polarization of the reaction product, and comparing it with the fluorescence polarization value of fluorescent probe itself. A method for screening inhibitors against formation of a protein complex comprises the steps of: (1) reacting the HIF-1alpha C-terminal peptides with CBP or p300 protein and measuring the fluorescence polarization of the reaction product; (2) adding a candidate inhibitor into the mixture and measuring the fluorescence polarization of the mixture; and (3) comparing the fluorescence polarization values of steps (1) and (2), and deciding that the candidate material as an inhibitor against the protein complex when the fluorescence polarization value of step (2) is smaller than that of step (1).
Abstract translation: 提供HIF-1αC-末端肽和CBP（环AMP应答元件结合蛋白）或p300蛋白之间相互作用的定量分析方法，通过去除HIF-1αC蛋白复合物的分离来降低分析成本 - 末端肽和CBP或p300蛋白质，自动化与孔板，筛选大量的蛋白质复合物抑制剂，并分析HIF-1α翻译后修饰的功效。 用于定量分析HIF-1αC-末端肽和CBP或p300蛋白质之间的相互作用的方法包括以下步骤：（1）将荧光材料附着于HIF-1αC-末端肽以制备荧光探针; （2）使荧光标记的HIF-1αC-末端肽与CBP或p300蛋白质接触，使荧光材料与CBP或p300反应; 和（3）测量反应产物的荧光偏振，并将其与荧光探针本身的荧光偏振值进行比较。 用于筛选抗蛋白质复合物形成抑制剂的方法包括以下步骤：（1）使HIF-1αC-末端肽与CBP或p300蛋白质反应并测量反应产物的荧光极化; （2）将候选抑制剂加入到混合物中并测量混合物的荧光偏振; （3）比较步骤（1）和（2）的荧光偏振值，并且当步骤（2）的荧光偏振值小于步骤（1）的荧光偏振值小于蛋白质复合物的抑制剂时， ）。
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Patent Agency Ranking
公开(公告)号：KR101917287B1
公开(公告)日：2018-11-13
申请号：KR1020160173625
申请日：2016-12-19
Applicant: 한국과학기술연구원
IPC: C12N15/113 , C12N9/22 , C12Q1/68 , A61K47/48 , B82Y5/00
CPC classification number: A61K48/0058 , A61K9/14 , B82Y5/00 , C12N15/111 , C12N15/113 , C12N2310/14 , C12N2310/141 , C12N2310/20 , C12N2310/3519 , C12N2320/32
Abstract: 본발명은 Single guide RNA(sgRNA) 영역과 small interfering RNA(siRNA) 영역을갖는복수개의제1 반복단위를포함하는폴리리보뉴클레오티드; 및하나이상의상기 sgRNA 영역에결합된뉴클레아제단백질을포함하는, 스스로조립되는폴리리보뉴클레오티드-단백질복합체및 그용도에관한것이다.
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公开(公告)号：KR101793172B1
公开(公告)日：2017-11-03
申请号：KR1020160127689
申请日：2016-10-04
Applicant: 한국과학기술연구원
CPC classification number: C01G7/00 , A61K9/5115 , B82Y40/00 , C01P2004/64 , C09C3/10 , C12N15/11
Abstract: 본발명은금속나노입자코어층, 및상기코어층 표면에 L-DNA, 2’-(C-C알콕시)-RNA, 및 2’-할로-RNA로이루어진군으로부터선택된 1종이상의단일가닥의변형핵산(modified nucleic acid)이포함되어있는쉘 층을포함하는코어-쉘구조의금속나노입자및 이의제조방법에관한것이다. 본발명에따른코어-쉘구조의금속나노입자는세포에독성이없으면서도세포내 흡수효율이매우우수하여, 다양한나노메디신(nano medicine) 및약물전달기술에활용될수 있다.
Abstract translation: 本发明是一种金属纳米颗粒核层，核心层和L-DNA，2”的表面 - （CC烷氧基）-RNA，和单链的修饰的核酸一个成员上选自2'-卤代-RNA的（的组中选择 修饰的核酸）和生产纳米颗粒的方法。 根据本发明的核 - 壳结构的金属纳米颗粒具有优异的细胞内吸收效率而不会对细胞有毒，并且可以用于各种纳米药物和药物递送技术。
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公开(公告)号：KR101647804B1
公开(公告)日：2016-08-11
申请号：KR1020150089967
申请日：2015-06-24
Applicant: 한국과학기술연구원
Abstract: 본발명은신규한세포투과펩타이드및 이의용도에관한것이다. 본발명의펩타이드는적은수의아미노산(구체적으로는, 4개의아미노산)으로구성되며소수성아미노산의비중이높아분해효소에의해잘 분해되지않고우수한세포투과성을가진다. 또한, 본발명의펩타이드의양 말단, 특히 C-말단에목적카르고와결합되어상기목적카르고(예컨대, siRNA)를안정적이고효과적으로세포내(세포질)로전달할수 있다. 따라서, 본발명의펩타이드를포함하는약물전달체, 그리고이를이용하는목적카르고의전달방법및 조성물은질병또는질환의치료, 특정세포의검출, 질병(예컨대, 암)의진단, 특정세포의위치추적및이미징, 등에이용될수 있다.
Abstract translation: 本发明涉及新型细胞穿透肽及其用途。 该肽由少量氨基酸（更具体地说是四个氨基酸）组成，并且具有高比例的疏水性氨基酸，因此几乎不被蛋白酶降解并具有优异的细胞渗透性。 此外，本发明的肽的两端，特别是c末端与目标载体偶联，因此肽可以将目标载体（例如siRNA）稳定且有效地转移到细胞（细胞质）中 。 因此，含有本发明的肽的药物载体，使用其的药物转移方法和含有该肽的组合物可以用于疾病或疾病的治疗，特异性细胞的检测，疾病的诊断（对于 例如癌症），跟踪特定细胞的位置，体内成像等
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公开(公告)号：KR101485389B1
公开(公告)日：2015-01-26
申请号：KR1020130024657
申请日：2013-03-07
Applicant: 한국과학기술연구원
CPC classification number: A61K49/0093 , A61K49/0002 , A61K49/0032 , A61K49/0054
Abstract: 본 발명은 조영제 조성물 및 이를 이용한 바이오 영상화 방법에 관한 것이다. 본 발명의 조영제 조성물은 생분자인 DNA 나노 구조체를 주요 성분으로 포함하기 때문에, 본질적으로 비세포독성 및 비면역성이며, 다른 유기 또는 무기 기반 조영제 조성물에서 관찰될 수 있는 안전성 문제를 야기할 가능성이 낮다. 또한, 본 발명의 조영제 조성물은 우수한 세포 내 섭취 및 안정성을 나타내어 충분한 조영 증강 효과를 통해 질병의 진단을 용이하게 할 뿐만 아니라, 기존에 탐색이 어려웠던 감시림프절까지 영상화할 수 있기 때문에, 암의 전이 여부를 용이하게 판단하고, 저침습적 치료의 임상 적용을 가능하게 한다.
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公开(公告)号：KR1020140110401A
公开(公告)日：2014-09-17
申请号：KR1020130024657
申请日：2013-03-07
Applicant: 한국과학기술연구원
CPC classification number: A61K49/0093 , A61K49/0002 , A61K49/0032 , A61K49/0054
Abstract: The present invention relates to a contrast medium composition and a method of bio imagination using the same. The contrast medium composition of the present invention includes DNA nanostructure, which is a biomolecule, as an active ingredient; is fundamentally non-cytotoxic and non-immunogenic; and is not likely to induce safety problems that may be observed in other organic or inorganic contrast medium composition. In addition, the contrast medium composition of the present invention not only facilitates diagnosis of disease through sufficient contrast enhancement effect by showing excellent cellular uptake and intracellular stability but can visualize even SLNs which is difficult to be visualized traditionally, thus enabling easy examination of cancer metastasis and application of lower invasive treatment.
Abstract translation: 本发明涉及造影剂组合物和使用其的生物想象力的方法。 本发明的造影剂组合物包含作为活性成分的作为生物分子的DNA纳米结构体; 基本上是非细胞毒性和非免疫原性的; 并且不可能引起在其它有机或无机造影剂组合物中可能观察到的安全问题。 此外，本发明的造影剂组合物不仅通过显示优异的细胞摄取和细胞内稳定性而能够通过充分的对比度增强效果来促进疾病的诊断，而且可以使传统上难以显现的SLN甚至可视化，因此能够容易地检查癌症转移 并应用低侵入性治疗。
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