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公开(公告)号：KR1020130124020A
公开(公告)日：2013-11-13
申请号：KR1020120047603
申请日：2012-05-04
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/48 , C12Y203/01054 , G01N27/447 , G01N33/582 , G01N2333/91057
Abstract: The present invention relates to a kit for detecting Escherichia coli, containing fluorescent compounds which specifically bind to pyruvate formate-lyase activating enzyme expressed from ybiY gene of Escherichia coli and a method for detecting Escherichia coli using the same. According to a specific example, the method and the kit easily, quickly, and accurately provide identification results by detecting pyruvate formate-lyase activating enzyme expressed from ybiY gene of Escherichia coli which specifically binds to 3',6'-bis(acetyl oxy)-5-(chloromethyl)-spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-3-one and distinguishing Escherichia coli and Enterococcus faecalis.
Abstract translation: 本发明涉及用于检测大肠杆菌的试剂盒，其含有特异性结合由大肠杆菌的ybiY基因表达的丙酮酸甲酸裂解酶活化酶的荧光化合物和使用其的大肠杆菌检测方法。 根据具体实例，方法和试剂盒通过检测特异性结合3'，6'-双（乙酰氧基）乙酸的大肠杆菌的ybiY基因表达的丙酮酸甲酸裂解酶活化酶，容易，快速并准确地提供鉴定结果。 -5-（氯甲基） - 螺（异苯并呋喃-1（3H），9' - （9H）呫吨）-3-酮，区分大肠杆菌和粪肠球菌。
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公开(公告)号：KR1020130124016A
公开(公告)日：2013-11-13
申请号：KR1020120047599
申请日：2012-05-04
Applicant: 한국과학기술연구원
CPC classification number: Y02A50/451 , C12Q1/26 , C12Y102/01002 , G01N27/447 , G01N33/582 , G01N2333/90203
Abstract: The present invention relates to a kit for detecting Enterobacter cloacae, containing fluorescent compounds which specifically bind to formate hydrogen lyase expressed from hycB gene of Enterobacter cloacae and a method for detecting Enterobacter cloacae using the same. According to a specific example, the method and the kit of the present invention easily, quickly, and accurately provide identification results by detecting formate hydrogen lyase expressed from hycB gene of Enterobacter cloacae and distinguishing Enterobacter cloacae and Escherichia coli.
Abstract translation: 本发明涉及一种用于检测阴沟肠杆菌的试剂盒，其包含特异性结合由阴沟肠杆菌的hycB基因表达的甲酸氢裂解酶的荧光化合物，以及使用该肠杆菌阴沟肠杆菌检测肠杆菌的方法。 根据具体实例，本发明的方法和试剂盒通过检测由阴沟肠杆菌的hycB基因和区分阴沟肠杆菌和大肠杆菌表达的甲酸氢裂解，容易，快速且准确地提供鉴定结果。
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公开(公告)号：KR1020130124015A
公开(公告)日：2013-11-13
申请号：KR1020120047598
申请日：2012-05-04
Applicant: 한국과학기술연구원
CPC classification number: C12Q1/485 , C12Y207/01035 , G01N27/447 , G01N33/582
Abstract: The present invention relates to a kit for detecting Bacillus subtilis, containing fluorescent compounds which specifically bind to pyridoxine kinase which is expressed from thiD gene of Bacillus subtilis and a method for detecting Bacillus subtilis using the same. According to a specific example, the method and the kit easily, quickly, and accurately provide identification results by detecting pyridoxine kinase expressed from thidD gene of Bacillus subtilis, which specifically binds to 3',6'-bis(acetyloxy)-5-(chlromethyl)-spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-3-one and distinguishing Bacillus subtilis and Bacillus cereus.
Abstract translation: 本发明涉及一种用于检测枯草芽孢杆菌的试剂盒，其含有与枯草芽孢杆菌的thiD基因表达的吡哆醇激酶特异性结合的荧光化合物和使用其的枯草芽孢杆菌检测方法。 根据具体实例，方法和试剂盒通过检测从枯草芽孢杆菌thidD基因表达的吡哆醇激酶容易，快速和准确地提供鉴定结果，其特异性结合3'，6'-双（乙酰氧基）-5-（ 甲基） - 螺（异苯并呋喃-1（3H），9' - （9H）呫吨）-3-酮，区分枯草芽孢杆菌和蜡状芽孢杆菌。
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公开(公告)号：KR1020130124014A
公开(公告)日：2013-11-13
申请号：KR1020120047597
申请日：2012-05-04
Applicant: 한국과학기술연구원
CPC classification number: G01N33/5767 , G01N27/447 , G01N33/56983 , G01N33/582 , G01N2333/186
Abstract: The present invention relates to a method for distinguishing hepatitis C virus injected liver cells and normal cells using fluorescent compounds. According to a specific example, the method easily, quickly, and accurately provides identification result by comparing the pattern of proteins which are conjugated to 2-(6-hydroxy-3-oxo-3H-xanthene-9-yl)-5-((2-(vinylsulfonyl)ethyl)carbamoyl)benzoic acid and 2-(6-hydroxy-3-oxo-3H-xanthene-9-yl)-4-((2-(vinylsulfonyl)ethyl)carbamoyl)benzoic acid and distinguishing hepatitis C virus infected liver cells and normal cells.
Abstract translation: 本发明涉及使用荧光化合物区分丙肝病毒注射的肝细胞和正常细胞的方法。 根据具体实例，该方法通过比较与2-（6-羟基-3-氧代-3H-呫吨-9-基）-5-（ （2-（乙烯基磺酰基）乙基）氨基甲酰基）苯甲酸和2-（6-羟基-3-氧代-3H-呫吨-9-基）-4 - （（2-（乙烯基磺酰基）乙基）氨基甲酰基）苯甲酸， 丙型肝炎病毒感染肝细胞和正常细胞。
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25.发明授权
公开(公告)号：KR101673553B1
公开(公告)日：2016-11-07
申请号：KR1020130131501
申请日：2013-10-31
Applicant: 한국과학기술연구원
Abstract: 본발명은단실유도체의새로운용도에대한것으로, 구체적으로단실유도체가활성산소종을검출하는데유용하게활용될수 있음을밝힌것이다. 본발명의제조방법및 이를통해제조된다양한단실유도체는활성산소종과접촉시방출파장이변화하므로, 이를통하여활성산소종의존재여부를검출하는데유용하게활용될수 있다. 따라서, 이러한분자들은시료내존재하는활성산소종의존재를용이하게이미지화하므로, 다양한연구및 산업적인용도로활용될수 있다.
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Patent Agency Ranking
公开(公告)号：KR101494881B1
公开(公告)日：2015-02-24
申请号：KR1020130050848
申请日：2013-05-06
Applicant: 한국과학기술연구원
Abstract: 본 발명은 바실러스 서브틸리스의
ywbO 유전자로부터 발현되는 설퍼 옥시도리덕타아제와 특이적으로 결합하는 형광 화합물을 포함하는 바실러스 서브틸리스 검출 키트 및 이를 이용한 바실러스 서브틸리스 검출 방법에 관한 것이다. 일 구체예에 따르면, 본 발명의 방법 및 키트는 3',6'-비스(아세틸옥시)-5-(클로로메틸)-스피로(이소벤조퓨란-1(3H),9'-(9H)잔텐)-3-온에 특이적으로 결합하는 바실러스 서브틸리스의
ywbO 유전자로부터 발현되는 설퍼 옥시도리덕타아제를 검출하여 바실러스 서브틸리스와 엔테로코쿠스 패칼리스를 구별하므로, 쉽고 빠르며 정확한 동정 결과를 제공할 수 있다.-
公开(公告)号：KR101345797B1
公开(公告)日：2013-12-27
申请号：KR1020120047597
申请日：2012-05-04
Applicant: 한국과학기술연구원
Abstract: 본발명은형광화합물을이용하여헤파티티스 C 바이러스가감염된간세포와정상세포를구별하는방법에관한것이다. 일구체예에따르면, 본발명의방법은 2-(6-히드록시-3-옥소-3H-잔텐-9-일)-5-((2-(비닐술포닐)에틸)카바모일)벤조산및 2-(6-히드록시-3-옥소-3H-잔텐-9-일)-4-((2-(비닐술포닐)에틸)카바모일)벤조산에결합하는단백질패턴을비교하여헤파티티스 C 바이러스가감염된간세포와정상세포를구별하므로, 쉽고빠르며정확한동정결과를제공할수 있다.
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公开(公告)号：KR101343351B1
公开(公告)日：2013-12-20
申请号：KR1020120047595
申请日：2012-05-04
Applicant: 한국과학기술연구원
Abstract: 본발명은형광화합물을이용하여엔테로박터클로아세및 에스케리키아콜라이를구별하는방법에관한것이다. 일구체예에따르면, 본발명의방법은 3',6'-비스(아세틸옥시)-5-(클로로메틸)-스피로(이소벤조퓨란-1(3H),9'-(9H)잔텐)-3-온에결합하는단백질패턴을비교하여엔테로박터클로아세및 에스케리키아콜라이를구별하므로, 쉽고빠르며정확한동정결과를제공할수 있다.
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公开(公告)号：KR1020130124017A
公开(公告)日：2013-11-13
申请号：KR1020120047600
申请日：2012-05-04
Applicant: 한국과학기술연구원
CPC classification number: Y02A50/451 , C12Q1/48 , C12Y207/01011 , G01N27/447 , G01N33/582 , G01N2333/91215
Abstract: The present invention relates to a detection kit for Enterobacter cloacae including fluorophores which are specifically bonded to 6-phosphofructokinase expressed from pfkB gene of the Enterobacter cloacae and a method for detecting Enterobacter cloacae using the same. According to one specific example, the method and kit of the present invention provide easy, rapid and accurate identification by detecting 6-phosphofructokinase expressed from the pfkB gene of the Enterobacter cloacae which specifically bonds to 3',6'-bis(acetyloxy)-5-(chloromethyl)-spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one and distinguishing Enterobactercloacae from Enterococcusfaecalis.
Abstract translation: 本发明涉及一种包括荧光团的肠道杆菌检测试剂盒，其特征在于与阴道肠杆菌的pfkB基因表达的6-磷酸果糖激酶特异性结合，以及使用该荧光团检测阴道肠杆菌的方法。 根据一个具体实例，本发明的方法和试剂盒通过检测特异性结合到3'，6'-双（乙酰氧基） - 苯甲酸的肠杆菌阴沟肠杆菌的pfkB基因表达的6-磷酸果糖激酶提供了容易，快速和准确的鉴定。 5-（氯甲基） - 螺（异苯并呋喃-1（3H），9' - （9H）呫吨）-3-酮，并将肠杆菌与肠球菌区分开。
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公开(公告)号：KR1020130124012A
公开(公告)日：2013-11-13
申请号：KR1020120047595
申请日：2012-05-04
Applicant: 한국과학기술연구원
Abstract: The present invention relates to a method for distinguishing Enterobacter cloacae and Escherichia coli using fluorescent compounds. According to a specific example, the method easily, quickly, and accurately provides identification results by comparing the pattern of proteins which are conjugated to 3’,6’-bis(acetyloxy)-5-(chloromethyl)-spiro(isobenzofuran-1(3H),9’-(9H)xanthene)-3-one and distinguishing Enterobacter cloacae and Escherichia coli.
Abstract translation: 本发明涉及使用荧光化合物区分阴道肠杆菌和大肠杆菌的方法。 根据具体实例，该方法通过比较与3'，6'-（双（乙酰氧基）-5-（氯甲基） - 螺（异苯并呋喃-1（ 3H），9“ - （9H）呫吨）-3-one和区分阴沟肠杆菌和大肠杆菌。
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