公开(公告)号：EP3204054A1

公开(公告)日：2017-08-16

申请号：EP15784217.0

申请日：2015-10-06

Applicant: 3M Innovative Properties Company

Inventor： SWAMINATHAN, Swarnalatha ,  BENNAARS-EIDEN, Assumpta A. G. ,  AHIMOU, Francois ,  FOLTZ, William E. ,  ZHANG, Ying

IPC: A61L2/28 ,  C12M1/34

CPC classification number: A61L2/28 ,  C12M1/34 ,  C12M37/06 ,  C12Q1/22 ,  G01N31/226

Abstract: A self-contained biological sterilization indicator is provided. The self-contained biological sterilization indicator includes an outer container having at least one liquid-impermeable wall and an interior volume; a sealed, openable, liquid-impermeable inner container enclosing a predetermined volume of an aqueous medium; a dry coating that comprises i) a plurality of viable test microorganisms useful to detect exposure to an oxidative sterilant and ii) an effective amount of a sterilant-resistance modulator; and a pathway that permits vapor communication between the interior volume and an atmosphere outside the outer container. The inner container and the dry coating are disposed in the interior volume. The modulator comprises an amino acid. The effective amount causes an increase in sensitivity of the test microorganisms to the oxidative sterilant relative to an otherwise-identical dry coating that lacks the effective amount.

Abstract translation: 提供独立的生物灭菌指示器。 独立式生物灭菌指示器包括具有至少一个液体不可渗透的壁和内部容积的外部容器; 封闭预定体积的水性介质的密封的，可打开的不透液体的内部容器; 干燥涂层，其包含i）多种用于检测暴露于氧化性杀菌剂的活性测试微生物，和ii）有效量的杀菌剂抗性调节剂; 以及允许内部容积与外部容器外部的气氛之间的蒸汽连通的路径。 内部容器和干燥涂层设置在内部容积中。 该调节剂包含氨基酸。 相对于缺乏有效量的另外相同的干涂层，有效量导致测试微生物对氧化消毒剂的敏感性增加。

公开(公告)号：EP2814977A1

公开(公告)日：2014-12-24

申请号：EP13707735.0

申请日：2013-02-11

Applicant: 3M Innovative Properties Company

Inventor： AHIMOU, Francois ,  CHANDRAPATI, Sailaja ,  HALVERSON, Kurt J. ,  WEBB, Heather M. ,  WILLIAMS, Michael G.

IPC: C12Q1/22

CPC classification number: C12Q1/22 ,  G01N21/6428 ,  G01N2201/061

Abstract: A biological sterilization indicator device is provided. The device comprises a body, a plurality of test microorganisms, and an oxygen-modulated first fluorescent sensor. The body comprises a first layer attached to a second layer, forming at least one isolatable microchamber and at least one primary passageway that provides fluidic communication between ambience and the at least one microchamber. The microchamber has an isolated volume of about 0.5 microliters to about 9.5 microliters. The plurality of test microorganisms and the oxygen-modulated first fluorescent sensor are disposed in the microchamber. A method of using the device to determine the effectiveness of a sterilization process is also provided.

Abstract translation: 生物灭菌指示器装置。 该装置包括主体，试验微生物的多个，并且对氧的调制的第一荧光传感器。 所述主体包括附接到第二层上的第一层，形成至少一个伊索拉表微室和至少一个主通道确实提供氛围和所述至少一个微室之间的流体连通。 所述微腔具有在约0.5微升隔离容积至约9.5微升。 测试微生物和氧调制的第一荧光传感器的多元性被布置在微室。 因此提供了一种使用该设备来确定矿灭菌过程的有效性的方法。